Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01755 and BWI76_RS01760 overlap by 4 nucleotides BWI76_RS01760 and BWI76_RS01765 are separated by 185 nucleotides BWI76_RS01765 and BWI76_RS01770 are separated by 111 nucleotides
BWI76_RS01755: BWI76_RS01755 - 23S rRNA pseudouridine synthase F, at 358,763 to 359,635
_RS01755
BWI76_RS01760: BWI76_RS01760 - hypothetical protein, at 359,632 to 359,904
_RS01760
BWI76_RS01765: BWI76_RS01765 - hypothetical protein, at 360,090 to 361,031
_RS01765
BWI76_RS01770: BWI76_RS01770 - transcriptional regulatory protein RtcR, at 361,143 to 362,723
_RS01770
Position (kb) 360
361
362 Strain fitness (log2 ratio) -2
-1
0
1
2 at 359.091 kb on + strand, within BWI76_RS01755 at 359.191 kb on + strand, within BWI76_RS01755 at 359.252 kb on - strand, within BWI76_RS01755 at 359.896 kb on - strand at 359.900 kb on + strand at 359.922 kb on + strand at 359.930 kb on - strand at 359.943 kb on - strand at 360.027 kb on - strand at 360.134 kb on + strand at 360.339 kb on - strand, within BWI76_RS01765 at 360.349 kb on + strand, within BWI76_RS01765 at 360.367 kb on + strand, within BWI76_RS01765 at 360.417 kb on - strand, within BWI76_RS01765 at 360.518 kb on + strand, within BWI76_RS01765 at 360.527 kb on - strand, within BWI76_RS01765 at 360.553 kb on + strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765 at 360.671 kb on - strand, within BWI76_RS01765 at 360.740 kb on + strand, within BWI76_RS01765 at 360.895 kb on + strand, within BWI76_RS01765 at 361.073 kb on - strand at 361.073 kb on - strand at 361.086 kb on + strand at 361.171 kb on - strand at 361.393 kb on + strand, within BWI76_RS01770 at 361.399 kb on - strand, within BWI76_RS01770 at 361.532 kb on + strand, within BWI76_RS01770 at 361.809 kb on - strand, within BWI76_RS01770 at 361.815 kb on + strand, within BWI76_RS01770 at 361.874 kb on - strand, within BWI76_RS01770 at 361.929 kb on - strand, within BWI76_RS01770 at 361.965 kb on - strand, within BWI76_RS01770
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 359,091 + BWI76_RS01755 0.38 -2.7 359,191 + BWI76_RS01755 0.49 -0.3 359,252 - BWI76_RS01755 0.56 -0.0 359,896 - +1.7 359,900 + +1.1 359,922 + +0.9 359,930 - +0.6 359,943 - +0.0 360,027 - -2.2 360,134 + -0.5 360,339 - BWI76_RS01765 0.26 +0.1 360,349 + BWI76_RS01765 0.27 +0.6 360,367 + BWI76_RS01765 0.29 -0.8 360,417 - BWI76_RS01765 0.35 -0.9 360,518 + BWI76_RS01765 0.45 -0.3 360,527 - BWI76_RS01765 0.46 -0.6 360,553 + BWI76_RS01765 0.49 -0.6 360,561 - BWI76_RS01765 0.50 -0.5 360,561 - BWI76_RS01765 0.50 -0.3 360,671 - BWI76_RS01765 0.62 -0.9 360,740 + BWI76_RS01765 0.69 -0.4 360,895 + BWI76_RS01765 0.85 -1.2 361,073 - -0.2 361,073 - -1.1 361,086 + -0.1 361,171 - +0.9 361,393 + BWI76_RS01770 0.16 -1.1 361,399 - BWI76_RS01770 0.16 +0.1 361,532 + BWI76_RS01770 0.25 +1.6 361,809 - BWI76_RS01770 0.42 +1.1 361,815 + BWI76_RS01770 0.43 +1.8 361,874 - BWI76_RS01770 0.46 -0.4 361,929 - BWI76_RS01770 0.50 -0.1 361,965 - BWI76_RS01770 0.52 -0.6
Or see this region's nucleotide sequence