Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotides BWI76_RS01715 and BWI76_RS01720 overlap by 8 nucleotides BWI76_RS01720 and BWI76_RS01725 are separated by 123 nucleotides BWI76_RS01725 and BWI76_RS01730 are separated by 11 nucleotides
BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673
_RS01710
BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514
_RS01715
BWI76_RS01720: BWI76_RS01720 - L-sorbose 1-phosphate reductase (EC 1.1.1.-) <small>(from data)</small>, at 352,507 to 353,736
_RS01720
BWI76_RS01725: BWI76_RS01725 - PTS mannose/fructose/sorbose family transporter subunit IID, at 353,860 to 354,684
_RS01725
BWI76_RS01730: BWI76_RS01730 - PTS mannose/fructose/sorbose transporter subunit IIC, at 354,696 to 355,493
_RS01730
Position (kb) 352
353
354 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 351.622 kb on + strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.635 kb on - strand, within BWI76_RS01710 at 351.682 kb on + strand at 351.682 kb on + strand at 351.690 kb on - strand at 351.722 kb on - strand at 351.777 kb on + strand at 351.834 kb on + strand, within BWI76_RS01715 at 351.988 kb on - strand, within BWI76_RS01715 at 351.999 kb on - strand, within BWI76_RS01715 at 352.145 kb on - strand, within BWI76_RS01715 at 352.218 kb on - strand, within BWI76_RS01715 at 352.249 kb on + strand, within BWI76_RS01715 at 352.280 kb on + strand, within BWI76_RS01715 at 352.309 kb on - strand, within BWI76_RS01715 at 352.346 kb on - strand, within BWI76_RS01715 at 352.391 kb on - strand, within BWI76_RS01715 at 352.392 kb on + strand, within BWI76_RS01715 at 352.416 kb on - strand, within BWI76_RS01715 at 352.486 kb on + strand at 352.687 kb on + strand, within BWI76_RS01720 at 352.722 kb on + strand, within BWI76_RS01720 at 352.724 kb on - strand, within BWI76_RS01720 at 352.930 kb on + strand, within BWI76_RS01720 at 353.173 kb on + strand, within BWI76_RS01720 at 353.189 kb on + strand, within BWI76_RS01720 at 353.450 kb on - strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.721 kb on + strand at 353.748 kb on + strand at 353.815 kb on + strand at 353.942 kb on - strand at 353.951 kb on + strand, within BWI76_RS01725 at 353.978 kb on - strand, within BWI76_RS01725 at 353.985 kb on + strand, within BWI76_RS01725 at 353.990 kb on + strand, within BWI76_RS01725 at 354.077 kb on - strand, within BWI76_RS01725 at 354.134 kb on - strand, within BWI76_RS01725 at 354.182 kb on + strand, within BWI76_RS01725 at 354.223 kb on + strand, within BWI76_RS01725 at 354.335 kb on + strand, within BWI76_RS01725 at 354.477 kb on + strand, within BWI76_RS01725 at 354.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 351,622 + BWI76_RS01710 0.84 +0.0 351,622 + BWI76_RS01710 0.84 +0.3 351,627 + BWI76_RS01710 0.85 -0.9 351,627 + BWI76_RS01710 0.85 +1.5 351,627 + BWI76_RS01710 0.85 -1.2 351,630 - BWI76_RS01710 0.86 -0.3 351,630 - BWI76_RS01710 0.86 +2.2 351,630 - BWI76_RS01710 0.86 +0.9 351,635 - BWI76_RS01710 0.88 -1.5 351,682 + -0.1 351,682 + -0.8 351,690 - -1.2 351,722 - +1.6 351,777 + +0.2 351,834 + BWI76_RS01715 0.16 +1.6 351,988 - BWI76_RS01715 0.35 -0.2 351,999 - BWI76_RS01715 0.36 -0.2 352,145 - BWI76_RS01715 0.54 +0.5 352,218 - BWI76_RS01715 0.63 -0.5 352,249 + BWI76_RS01715 0.67 -0.1 352,280 + BWI76_RS01715 0.71 -2.2 352,309 - BWI76_RS01715 0.75 -0.3 352,346 - BWI76_RS01715 0.79 +0.1 352,391 - BWI76_RS01715 0.85 +0.1 352,392 + BWI76_RS01715 0.85 +0.3 352,416 - BWI76_RS01715 0.88 +1.6 352,486 + +1.1 352,687 + BWI76_RS01720 0.15 +2.6 352,722 + BWI76_RS01720 0.17 +0.5 352,724 - BWI76_RS01720 0.18 -1.6 352,930 + BWI76_RS01720 0.34 +1.5 353,173 + BWI76_RS01720 0.54 +0.9 353,189 + BWI76_RS01720 0.55 -0.0 353,450 - BWI76_RS01720 0.77 -2.1 353,467 + BWI76_RS01720 0.78 +1.9 353,467 + BWI76_RS01720 0.78 +1.1 353,721 + -0.0 353,748 + +0.7 353,815 + -0.0 353,942 - -1.9 353,951 + BWI76_RS01725 0.11 -1.3 353,978 - BWI76_RS01725 0.14 +0.7 353,985 + BWI76_RS01725 0.15 -1.3 353,990 + BWI76_RS01725 0.16 -2.1 354,077 - BWI76_RS01725 0.26 +0.2 354,134 - BWI76_RS01725 0.33 -1.1 354,182 + BWI76_RS01725 0.39 -1.2 354,223 + BWI76_RS01725 0.44 -2.9 354,335 + BWI76_RS01725 0.58 +0.2 354,477 + BWI76_RS01725 0.75 -0.1 354,718 - -1.7
Or see this region's nucleotide sequence