Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01240 and BWI76_RS01245 are separated by 67 nucleotides BWI76_RS01245 and BWI76_RS01250 are separated by 61 nucleotides BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides
BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527
_RS01240
BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421
_RS01245
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
Position (kb) 254
255
256
257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 253.615 kb on - strand at 253.657 kb on + strand at 253.664 kb on + strand at 253.664 kb on + strand at 253.672 kb on - strand at 253.672 kb on - strand at 253.743 kb on + strand, within BWI76_RS01240 at 253.815 kb on + strand, within BWI76_RS01240 at 253.823 kb on - strand, within BWI76_RS01240 at 253.939 kb on - strand, within BWI76_RS01240 at 254.025 kb on - strand, within BWI76_RS01240 at 254.123 kb on + strand, within BWI76_RS01240 at 254.316 kb on + strand, within BWI76_RS01240 at 254.331 kb on + strand, within BWI76_RS01240 at 254.339 kb on - strand, within BWI76_RS01240 at 254.427 kb on + strand, within BWI76_RS01240 at 254.437 kb on + strand, within BWI76_RS01240 at 254.639 kb on + strand at 254.703 kb on + strand at 254.722 kb on + strand at 254.722 kb on + strand at 254.769 kb on - strand at 254.831 kb on + strand, within BWI76_RS01245 at 254.901 kb on - strand, within BWI76_RS01245 at 255.222 kb on - strand, within BWI76_RS01245 at 255.654 kb on - strand, within BWI76_RS01245 at 255.695 kb on + strand, within BWI76_RS01245 at 255.739 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.963 kb on + strand, within BWI76_RS01245 at 256.006 kb on + strand, within BWI76_RS01245 at 256.258 kb on + strand at 256.357 kb on - strand at 256.376 kb on - strand at 256.408 kb on + strand at 256.535 kb on + strand at 256.543 kb on - strand at 256.633 kb on - strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.651 kb on - strand, within BWI76_RS01250 at 256.656 kb on - strand, within BWI76_RS01250 at 256.718 kb on + strand, within BWI76_RS01250 at 256.807 kb on - strand, within BWI76_RS01250 at 256.875 kb on - strand, within BWI76_RS01250 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 253,615 - -0.7 253,657 + +0.8 253,664 + -0.1 253,664 + -0.4 253,672 - -0.7 253,672 - -2.0 253,743 + BWI76_RS01240 0.10 -1.3 253,815 + BWI76_RS01240 0.18 +2.2 253,823 - BWI76_RS01240 0.19 -1.6 253,939 - BWI76_RS01240 0.33 +1.0 254,025 - BWI76_RS01240 0.42 +1.9 254,123 + BWI76_RS01240 0.54 +1.6 254,316 + BWI76_RS01240 0.76 +0.4 254,331 + BWI76_RS01240 0.77 -0.1 254,339 - BWI76_RS01240 0.78 -2.5 254,427 + BWI76_RS01240 0.88 -2.4 254,437 + BWI76_RS01240 0.90 +1.4 254,639 + -2.3 254,703 + -1.9 254,722 + +1.2 254,722 + -0.7 254,769 - +0.7 254,831 + BWI76_RS01245 0.13 -3.8 254,901 - BWI76_RS01245 0.17 +0.0 255,222 - BWI76_RS01245 0.34 -0.2 255,654 - BWI76_RS01245 0.58 -1.2 255,695 + BWI76_RS01245 0.60 -1.0 255,739 + BWI76_RS01245 0.63 -0.5 255,885 + BWI76_RS01245 0.71 -2.3 255,885 + BWI76_RS01245 0.71 -2.9 255,885 + BWI76_RS01245 0.71 -0.4 255,963 + BWI76_RS01245 0.75 -0.0 256,006 + BWI76_RS01245 0.77 +0.7 256,258 + -0.1 256,357 - -0.3 256,376 - -1.0 256,408 + +0.9 256,535 + +1.0 256,543 - +2.8 256,633 - BWI76_RS01250 0.24 -0.6 256,643 + BWI76_RS01250 0.26 -0.5 256,643 + BWI76_RS01250 0.26 -0.7 256,651 - BWI76_RS01250 0.27 -0.4 256,656 - BWI76_RS01250 0.28 -0.2 256,718 + BWI76_RS01250 0.38 -1.1 256,807 - BWI76_RS01250 0.52 +5.3 256,875 - BWI76_RS01250 0.63 +0.2 257,129 + -1.9 257,137 - -1.1 257,179 + -0.4 257,379 - -0.7 257,379 - +0.1
Or see this region's nucleotide sequence