Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotides BWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotides BWI76_RS01010 and BWI76_RS01015 overlap by 1 nucleotides BWI76_RS01015 and BWI76_RS01020 are separated by 102 nucleotides
BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995
_RS01000
BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894
_RS01005
BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668
_RS01010
BWI76_RS01015: BWI76_RS01015 - nicotinamide riboside transporter PnuC, at 206,668 to 207,390
_RS01015
BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511
_RS01020
Position (kb) 205
206
207 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 204.936 kb on - strand, within BWI76_RS01000 at 204.948 kb on + strand, within BWI76_RS01000 at 204.977 kb on - strand at 204.977 kb on - strand at 205.053 kb on + strand at 205.053 kb on + strand at 205.145 kb on + strand at 205.163 kb on + strand at 205.163 kb on + strand at 205.165 kb on + strand at 205.165 kb on + strand at 205.166 kb on - strand at 205.240 kb on + strand at 205.414 kb on + strand, within BWI76_RS01005 at 205.423 kb on - strand, within BWI76_RS01005 at 205.529 kb on + strand, within BWI76_RS01005 at 205.561 kb on - strand, within BWI76_RS01005 at 205.632 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.938 kb on - strand at 206.028 kb on + strand, within BWI76_RS01010 at 206.077 kb on + strand, within BWI76_RS01010 at 206.081 kb on - strand, within BWI76_RS01010 at 206.201 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.993 kb on + strand, within BWI76_RS01015 at 207.049 kb on + strand, within BWI76_RS01015 at 207.203 kb on - strand, within BWI76_RS01015 at 207.373 kb on - strand at 207.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 204,936 - BWI76_RS01000 0.79 -0.7 204,948 + BWI76_RS01000 0.83 -2.9 204,977 - -1.9 204,977 - +0.8 205,053 + +0.5 205,053 + +0.9 205,145 + -1.5 205,163 + -0.7 205,163 + +1.2 205,165 + -1.4 205,165 + +0.4 205,166 - -0.5 205,240 + -0.5 205,414 + BWI76_RS01005 0.32 -1.9 205,423 - BWI76_RS01005 0.33 -0.8 205,529 + BWI76_RS01005 0.48 -0.1 205,561 - BWI76_RS01005 0.53 -0.7 205,632 - BWI76_RS01005 0.63 +0.0 205,812 - BWI76_RS01005 0.88 -0.7 205,812 - BWI76_RS01005 0.88 -0.1 205,938 - -1.9 206,028 + BWI76_RS01010 0.15 +1.3 206,077 + BWI76_RS01010 0.21 +0.9 206,081 - BWI76_RS01010 0.22 -1.3 206,201 + BWI76_RS01010 0.38 +5.7 206,456 + BWI76_RS01010 0.72 +0.7 206,456 + BWI76_RS01010 0.72 +1.4 206,464 - BWI76_RS01010 0.73 +1.2 206,464 - BWI76_RS01010 0.73 -0.2 206,464 - BWI76_RS01010 0.73 +1.9 206,993 + BWI76_RS01015 0.45 -0.0 207,049 + BWI76_RS01015 0.53 -1.0 207,203 - BWI76_RS01015 0.74 +1.1 207,373 - +1.6 207,399 + +0.2
Or see this region's nucleotide sequence