Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00725

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00715 and BWI76_RS00720 overlap by 4 nucleotidesBWI76_RS00720 and BWI76_RS00725 are separated by 149 nucleotidesBWI76_RS00725 and BWI76_RS00730 are separated by 149 nucleotides BWI76_RS00715: BWI76_RS00715 - two-component sensor histidine kinase, at 145,529 to 146,902 _RS00715 BWI76_RS00720: BWI76_RS00720 - DNA-binding response regulator, at 146,899 to 147,597 _RS00720 BWI76_RS00725: BWI76_RS00725 - periplasmic repressor CpxP, at 147,747 to 148,250 _RS00725 BWI76_RS00730: BWI76_RS00730 - cation-efflux pump FieF, at 148,400 to 149,296 _RS00730 Position (kb) 147 148 149Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 147.037 kb on + strand, within BWI76_RS00720at 147.129 kb on + strand, within BWI76_RS00720at 147.356 kb on - strand, within BWI76_RS00720at 147.524 kb on - strand, within BWI76_RS00720at 147.661 kb on + strandat 147.669 kb on - strandat 147.669 kb on - strandat 147.861 kb on - strand, within BWI76_RS00725at 147.897 kb on - strand, within BWI76_RS00725at 148.215 kb on + strandat 148.368 kb on + strandat 148.411 kb on - strandat 148.416 kb on - strandat 148.431 kb on - strandat 148.453 kb on + strandat 148.500 kb on - strand, within BWI76_RS00730at 148.503 kb on - strand, within BWI76_RS00730at 148.575 kb on - strand, within BWI76_RS00730at 148.610 kb on - strand, within BWI76_RS00730at 148.742 kb on + strand, within BWI76_RS00730at 148.743 kb on + strand, within BWI76_RS00730at 148.774 kb on + strand, within BWI76_RS00730at 148.782 kb on - strand, within BWI76_RS00730at 148.942 kb on - strand, within BWI76_RS00730at 148.992 kb on - strand, within BWI76_RS00730at 149.029 kb on + strand, within BWI76_RS00730

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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147,037 + BWI76_RS00720 0.20 +0.4
147,129 + BWI76_RS00720 0.33 +0.1
147,356 - BWI76_RS00720 0.65 +2.5
147,524 - BWI76_RS00720 0.89 +3.0
147,661 + +2.3
147,669 - -0.1
147,669 - +0.0
147,861 - BWI76_RS00725 0.23 -1.9
147,897 - BWI76_RS00725 0.30 -0.1
148,215 + -0.4
148,368 + +0.4
148,411 - +4.9
148,416 - +0.9
148,431 - +0.1
148,453 + +0.2
148,500 - BWI76_RS00730 0.11 -1.5
148,503 - BWI76_RS00730 0.11 -1.0
148,575 - BWI76_RS00730 0.20 -0.2
148,610 - BWI76_RS00730 0.23 -0.1
148,742 + BWI76_RS00730 0.38 -0.6
148,743 + BWI76_RS00730 0.38 -0.1
148,774 + BWI76_RS00730 0.42 -0.3
148,782 - BWI76_RS00730 0.43 -0.7
148,942 - BWI76_RS00730 0.60 +0.1
148,992 - BWI76_RS00730 0.66 +0.3
149,029 + BWI76_RS00730 0.70 -0.7

Or see this region's nucleotide sequence