Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00305

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00300 and BWI76_RS00305 are separated by 3 nucleotidesBWI76_RS00305 and BWI76_RS00310 are separated by 476 nucleotides BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554 _RS00300 BWI76_RS00305: BWI76_RS00305 - GntR family transcriptional regulator, at 63,558 to 64,268 _RS00305 BWI76_RS00310: BWI76_RS00310 - 16S ribosomal RNA, at 64,745 to 66,298 _RS00310 Position (kb) 63 64 65Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 62.584 kb on - strand, within BWI76_RS00300at 62.599 kb on - strand, within BWI76_RS00300at 62.633 kb on + strand, within BWI76_RS00300at 62.673 kb on + strand, within BWI76_RS00300at 62.730 kb on - strand, within BWI76_RS00300at 62.814 kb on - strand, within BWI76_RS00300at 63.029 kb on - strand, within BWI76_RS00300at 63.075 kb on + strand, within BWI76_RS00300at 63.088 kb on - strand, within BWI76_RS00300at 63.098 kb on - strand, within BWI76_RS00300at 63.110 kb on - strand, within BWI76_RS00300at 63.115 kb on - strand, within BWI76_RS00300at 63.129 kb on - strand, within BWI76_RS00300at 63.129 kb on - strand, within BWI76_RS00300at 63.130 kb on - strand, within BWI76_RS00300at 63.404 kb on - strand, within BWI76_RS00300at 63.429 kb on + strandat 63.442 kb on - strandat 63.460 kb on + strandat 63.519 kb on - strandat 63.526 kb on + strandat 63.636 kb on + strand, within BWI76_RS00305at 63.668 kb on + strand, within BWI76_RS00305at 63.690 kb on - strand, within BWI76_RS00305at 63.706 kb on + strand, within BWI76_RS00305at 63.722 kb on - strand, within BWI76_RS00305at 63.811 kb on - strand, within BWI76_RS00305at 63.862 kb on + strand, within BWI76_RS00305at 63.862 kb on - strand, within BWI76_RS00305at 63.870 kb on - strand, within BWI76_RS00305at 63.923 kb on + strand, within BWI76_RS00305at 63.928 kb on + strand, within BWI76_RS00305at 63.931 kb on - strand, within BWI76_RS00305at 63.941 kb on - strand, within BWI76_RS00305at 63.962 kb on + strand, within BWI76_RS00305at 63.962 kb on - strand, within BWI76_RS00305at 63.980 kb on - strand, within BWI76_RS00305at 64.063 kb on + strand, within BWI76_RS00305at 64.076 kb on + strand, within BWI76_RS00305at 64.117 kb on - strand, within BWI76_RS00305at 64.150 kb on - strand, within BWI76_RS00305at 64.212 kb on - strandat 64.233 kb on - strandat 64.251 kb on - strandat 64.274 kb on - strandat 64.293 kb on + strandat 64.433 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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62,584 - BWI76_RS00300 0.31 -2.1
62,599 - BWI76_RS00300 0.32 +0.7
62,633 + BWI76_RS00300 0.34 -1.6
62,673 + BWI76_RS00300 0.37 -0.9
62,730 - BWI76_RS00300 0.41 -1.8
62,814 - BWI76_RS00300 0.47 -0.5
63,029 - BWI76_RS00300 0.62 +2.2
63,075 + BWI76_RS00300 0.66 +0.1
63,088 - BWI76_RS00300 0.67 +0.0
63,098 - BWI76_RS00300 0.67 +1.0
63,110 - BWI76_RS00300 0.68 -0.7
63,115 - BWI76_RS00300 0.69 -2.1
63,129 - BWI76_RS00300 0.70 +2.0
63,129 - BWI76_RS00300 0.70 -0.2
63,130 - BWI76_RS00300 0.70 +1.1
63,404 - BWI76_RS00300 0.89 -0.5
63,429 + +0.4
63,442 - +1.5
63,460 + -1.9
63,519 - +0.8
63,526 + +0.1
63,636 + BWI76_RS00305 0.11 +1.8
63,668 + BWI76_RS00305 0.15 +0.6
63,690 - BWI76_RS00305 0.19 +1.3
63,706 + BWI76_RS00305 0.21 +3.8
63,722 - BWI76_RS00305 0.23 +3.3
63,811 - BWI76_RS00305 0.36 +1.3
63,862 + BWI76_RS00305 0.43 +4.1
63,862 - BWI76_RS00305 0.43 +1.1
63,870 - BWI76_RS00305 0.44 +1.3
63,923 + BWI76_RS00305 0.51 +1.5
63,928 + BWI76_RS00305 0.52 +1.3
63,931 - BWI76_RS00305 0.52 +3.4
63,941 - BWI76_RS00305 0.54 +1.1
63,962 + BWI76_RS00305 0.57 -0.4
63,962 - BWI76_RS00305 0.57 +0.9
63,980 - BWI76_RS00305 0.59 +1.2
64,063 + BWI76_RS00305 0.71 -0.1
64,076 + BWI76_RS00305 0.73 +1.0
64,117 - BWI76_RS00305 0.79 -2.0
64,150 - BWI76_RS00305 0.83 +0.9
64,212 - +1.5
64,233 - -0.7
64,251 - +0.9
64,274 - +1.2
64,293 + +1.4
64,433 - +1.9

Or see this region's nucleotide sequence