Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22845 and BWI76_RS22850 are separated by 2 nucleotides BWI76_RS22850 and BWI76_RS22855 overlap by 1 nucleotides BWI76_RS22855 and BWI76_RS22860 are separated by 633 nucleotides
BWI76_RS22845: BWI76_RS22845 - flavodoxin, at 4,767,099 to 4,767,542
_RS22845
BWI76_RS22850: BWI76_RS22850 - tRNA pseudouridine synthase TruC, at 4,767,545 to 4,768,339
_RS22850
BWI76_RS22855: BWI76_RS22855 - hypothetical protein, at 4,768,339 to 4,768,668
_RS22855
BWI76_RS22860: BWI76_RS22860 - SecY-interacting protein, at 4,769,302 to 4,769,847
_RS22860
Position (kb) 4768
4769 Strain fitness (log2 ratio) -2
-1
0
1 at 4767.408 kb on + strand, within BWI76_RS22845 at 4767.889 kb on + strand, within BWI76_RS22850 at 4768.137 kb on + strand, within BWI76_RS22850 at 4768.291 kb on - strand at 4768.370 kb on + strand at 4768.428 kb on + strand, within BWI76_RS22855 at 4768.530 kb on + strand, within BWI76_RS22855 at 4768.538 kb on - strand, within BWI76_RS22855 at 4768.610 kb on - strand, within BWI76_RS22855 at 4768.748 kb on - strand at 4768.748 kb on - strand at 4768.803 kb on - strand at 4768.859 kb on - strand at 4768.902 kb on - strand at 4768.904 kb on + strand at 4768.985 kb on - strand at 4769.116 kb on + strand at 4769.378 kb on - strand, within BWI76_RS22860
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 4,767,408 + BWI76_RS22845 0.70 +0.3 4,767,889 + BWI76_RS22850 0.43 -0.2 4,768,137 + BWI76_RS22850 0.74 +0.1 4,768,291 - -0.1 4,768,370 + -1.5 4,768,428 + BWI76_RS22855 0.27 -2.6 4,768,530 + BWI76_RS22855 0.58 -1.3 4,768,538 - BWI76_RS22855 0.60 -1.5 4,768,610 - BWI76_RS22855 0.82 +0.3 4,768,748 - -0.6 4,768,748 - -0.4 4,768,803 - -2.4 4,768,859 - -0.8 4,768,902 - -0.4 4,768,904 + -0.9 4,768,985 - -1.4 4,769,116 + -0.2 4,769,378 - BWI76_RS22860 0.14 -0.9
Or see this region's nucleotide sequence