Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20850

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20840 and BWI76_RS20845 are separated by 78 nucleotidesBWI76_RS20845 and BWI76_RS20850 are separated by 58 nucleotidesBWI76_RS20850 and BWI76_RS20855 are separated by 186 nucleotidesBWI76_RS20855 and BWI76_RS20860 are separated by 5 nucleotides BWI76_RS20840: BWI76_RS20840 - hypothetical protein, at 4,356,046 to 4,357,113 _RS20840 BWI76_RS20845: BWI76_RS20845 - GDP-mannose pyrophosphatase NudK, at 4,357,192 to 4,357,776 _RS20845 BWI76_RS20850: BWI76_RS20850 - oxidoreductase FeS-binding subunit, at 4,357,835 to 4,359,799 _RS20850 BWI76_RS20855: BWI76_RS20855 - hypothetical protein, at 4,359,986 to 4,360,166 _RS20855 BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285 _RS20860 Position (kb) 4357 4358 4359 4360Strain fitness (log2 ratio) -3 -2 -1 0 1at 4356.930 kb on + strand, within BWI76_RS20840at 4357.582 kb on - strand, within BWI76_RS20845at 4357.656 kb on - strand, within BWI76_RS20845at 4357.656 kb on - strand, within BWI76_RS20845at 4357.807 kb on + strandat 4357.834 kb on - strandat 4358.128 kb on + strand, within BWI76_RS20850at 4358.624 kb on - strand, within BWI76_RS20850at 4358.896 kb on - strand, within BWI76_RS20850at 4358.900 kb on + strand, within BWI76_RS20850at 4359.006 kb on + strand, within BWI76_RS20850at 4359.093 kb on + strand, within BWI76_RS20850at 4359.150 kb on + strand, within BWI76_RS20850at 4359.155 kb on + strand, within BWI76_RS20850at 4359.321 kb on + strand, within BWI76_RS20850at 4359.350 kb on - strand, within BWI76_RS20850at 4359.636 kb on - strandat 4359.668 kb on - strandat 4359.670 kb on + strandat 4359.742 kb on + strandat 4359.983 kb on + strandat 4360.001 kb on + strandat 4360.009 kb on - strand, within BWI76_RS20855at 4360.011 kb on + strand, within BWI76_RS20855at 4360.013 kb on + strand, within BWI76_RS20855at 4360.025 kb on + strand, within BWI76_RS20855at 4360.033 kb on + strand, within BWI76_RS20855at 4360.098 kb on + strand, within BWI76_RS20855at 4360.100 kb on - strand, within BWI76_RS20855at 4360.106 kb on - strand, within BWI76_RS20855at 4360.106 kb on - strand, within BWI76_RS20855at 4360.118 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.120 kb on + strand, within BWI76_RS20855at 4360.128 kb on - strand, within BWI76_RS20855at 4360.235 kb on + strandat 4360.275 kb on + strandat 4360.367 kb on + strandat 4360.372 kb on + strandat 4360.417 kb on - strandat 4360.471 kb on + strandat 4360.525 kb on + strand, within BWI76_RS20860at 4360.583 kb on - strand, within BWI76_RS20860at 4360.707 kb on - strand, within BWI76_RS20860at 4360.747 kb on + strand, within BWI76_RS20860

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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4,356,930 + BWI76_RS20840 0.83 +0.8
4,357,582 - BWI76_RS20845 0.67 +0.3
4,357,656 - BWI76_RS20845 0.79 +0.3
4,357,656 - BWI76_RS20845 0.79 +0.0
4,357,807 + +0.5
4,357,834 - -0.7
4,358,128 + BWI76_RS20850 0.15 -0.0
4,358,624 - BWI76_RS20850 0.40 -3.3
4,358,896 - BWI76_RS20850 0.54 -1.6
4,358,900 + BWI76_RS20850 0.54 +1.1
4,359,006 + BWI76_RS20850 0.60 -0.2
4,359,093 + BWI76_RS20850 0.64 -0.9
4,359,150 + BWI76_RS20850 0.67 +0.0
4,359,155 + BWI76_RS20850 0.67 -0.5
4,359,321 + BWI76_RS20850 0.76 +0.3
4,359,350 - BWI76_RS20850 0.77 -3.7
4,359,636 - +0.7
4,359,668 - -0.8
4,359,670 + +0.1
4,359,742 + -0.1
4,359,983 + -0.9
4,360,001 + +0.3
4,360,009 - BWI76_RS20855 0.13 -0.5
4,360,011 + BWI76_RS20855 0.14 +0.7
4,360,013 + BWI76_RS20855 0.15 +0.3
4,360,025 + BWI76_RS20855 0.22 -0.2
4,360,033 + BWI76_RS20855 0.26 +0.5
4,360,098 + BWI76_RS20855 0.62 +0.6
4,360,100 - BWI76_RS20855 0.63 -0.3
4,360,106 - BWI76_RS20855 0.66 +0.3
4,360,106 - BWI76_RS20855 0.66 -0.3
4,360,118 + BWI76_RS20855 0.73 +0.3
4,360,120 + BWI76_RS20855 0.74 +0.1
4,360,120 + BWI76_RS20855 0.74 -0.3
4,360,120 + BWI76_RS20855 0.74 -0.6
4,360,128 - BWI76_RS20855 0.78 +0.6
4,360,235 + -1.7
4,360,275 + +0.4
4,360,367 + +0.6
4,360,372 + -0.2
4,360,417 - -0.1
4,360,471 + -0.3
4,360,525 + BWI76_RS20860 0.11 -0.5
4,360,583 - BWI76_RS20860 0.13 -0.0
4,360,707 - BWI76_RS20860 0.17 -0.8
4,360,747 + BWI76_RS20860 0.18 +0.7

Or see this region's nucleotide sequence