Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19365 and BWI76_RS19370 are separated by 438 nucleotides BWI76_RS19370 and BWI76_RS19375 are separated by 148 nucleotides BWI76_RS19375 and BWI76_RS19380 overlap by 4 nucleotides
BWI76_RS19365: BWI76_RS19365 - ribulokinase, at 4,035,482 to 4,037,089
_RS19365
BWI76_RS19370: BWI76_RS19370 - fructose-bisphosphate aldolase, at 4,037,528 to 4,038,580
_RS19370
BWI76_RS19375: BWI76_RS19375 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 4,038,729 to 4,039,529
_RS19375
BWI76_RS19380: BWI76_RS19380 - hydroxyethylthiazole kinase, at 4,039,526 to 4,040,299
_RS19380
Position (kb)
4037
4038
4039 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4036.947 kb on + strand at 4036.975 kb on + strand at 4037.014 kb on + strand at 4037.014 kb on + strand at 4037.014 kb on + strand at 4037.176 kb on - strand at 4037.176 kb on - strand at 4037.329 kb on - strand at 4037.397 kb on - strand at 4037.408 kb on + strand at 4037.571 kb on + strand at 4037.627 kb on + strand at 4037.820 kb on - strand, within BWI76_RS19370 at 4038.028 kb on - strand, within BWI76_RS19370 at 4038.091 kb on + strand, within BWI76_RS19370 at 4038.126 kb on - strand, within BWI76_RS19370 at 4038.128 kb on + strand, within BWI76_RS19370 at 4038.197 kb on + strand, within BWI76_RS19370 at 4038.347 kb on + strand, within BWI76_RS19370 at 4038.407 kb on + strand, within BWI76_RS19370 at 4038.413 kb on - strand, within BWI76_RS19370 at 4038.645 kb on + strand at 4038.645 kb on + strand at 4038.645 kb on + strand at 4038.645 kb on + strand at 4038.653 kb on - strand at 4038.653 kb on - strand at 4038.818 kb on - strand, within BWI76_RS19375 at 4038.976 kb on - strand, within BWI76_RS19375 at 4039.044 kb on + strand, within BWI76_RS19375 at 4039.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 4,036,947 + +2.3 4,036,975 + +3.2 4,037,014 + -0.3 4,037,014 + -1.2 4,037,014 + -0.9 4,037,176 - +0.9 4,037,176 - -0.5 4,037,329 - +0.3 4,037,397 - +1.1 4,037,408 + -0.4 4,037,571 + +1.1 4,037,627 + -0.5 4,037,820 - BWI76_RS19370 0.28 -0.1 4,038,028 - BWI76_RS19370 0.47 +0.6 4,038,091 + BWI76_RS19370 0.53 -0.1 4,038,126 - BWI76_RS19370 0.57 +0.3 4,038,128 + BWI76_RS19370 0.57 +0.0 4,038,197 + BWI76_RS19370 0.64 +0.9 4,038,347 + BWI76_RS19370 0.78 +0.6 4,038,407 + BWI76_RS19370 0.83 +0.1 4,038,413 - BWI76_RS19370 0.84 -0.8 4,038,645 + +1.9 4,038,645 + +2.5 4,038,645 + -0.5 4,038,645 + +0.2 4,038,653 - -0.5 4,038,653 - -0.0 4,038,818 - BWI76_RS19375 0.11 -2.1 4,038,976 - BWI76_RS19375 0.31 -1.3 4,039,044 + BWI76_RS19375 0.39 -0.0 4,039,493 - -0.0
Or see this region's nucleotide sequence