Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18020 and BWI76_RS18025 are separated by 832 nucleotides BWI76_RS18025 and BWI76_RS18030 are separated by 22 nucleotides BWI76_RS18030 and BWI76_RS18035 are separated by 18 nucleotides
BWI76_RS18020: BWI76_RS18020 - virulence factor VirK, at 3,744,377 to 3,745,321
_RS18020
BWI76_RS18025: BWI76_RS18025 - spore coat U domain-containing protein, at 3,746,154 to 3,746,705
_RS18025
BWI76_RS18030: BWI76_RS18030 - spore coat protein U, at 3,746,728 to 3,747,246
_RS18030
BWI76_RS18035: BWI76_RS18035 - fimbrial chaperone, at 3,747,265 to 3,748,029
_RS18035
Position (kb)
3746
3747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3745.277 kb on - strand at 3745.295 kb on + strand at 3745.324 kb on - strand at 3745.367 kb on - strand at 3745.369 kb on - strand at 3745.487 kb on + strand at 3745.523 kb on + strand at 3745.549 kb on - strand at 3745.554 kb on - strand at 3745.634 kb on + strand at 3745.642 kb on - strand at 3745.711 kb on + strand at 3745.722 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.738 kb on + strand at 3745.741 kb on - strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on - strand at 3745.754 kb on - strand at 3745.754 kb on - strand at 3745.767 kb on - strand at 3745.767 kb on - strand at 3745.777 kb on - strand at 3745.786 kb on - strand at 3745.791 kb on - strand at 3745.791 kb on - strand at 3745.791 kb on - strand at 3745.793 kb on + strand at 3745.801 kb on - strand at 3745.811 kb on - strand at 3745.811 kb on - strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.903 kb on + strand at 3745.984 kb on - strand at 3745.989 kb on - strand at 3746.062 kb on - strand at 3746.062 kb on - strand at 3746.093 kb on + strand at 3746.101 kb on - strand at 3746.147 kb on - strand at 3746.159 kb on + strand at 3746.159 kb on + strand at 3746.159 kb on + strand at 3746.179 kb on - strand at 3746.179 kb on - strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.240 kb on - strand, within BWI76_RS18025 at 3746.247 kb on - strand, within BWI76_RS18025 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.356 kb on - strand, within BWI76_RS18025 at 3746.404 kb on + strand, within BWI76_RS18025 at 3746.475 kb on + strand, within BWI76_RS18025 at 3746.598 kb on + strand, within BWI76_RS18025 at 3746.691 kb on + strand at 3746.916 kb on + strand, within BWI76_RS18030 at 3746.928 kb on + strand, within BWI76_RS18030 at 3746.938 kb on + strand, within BWI76_RS18030 at 3746.994 kb on + strand, within BWI76_RS18030 at 3747.017 kb on + strand, within BWI76_RS18030 at 3747.048 kb on + strand, within BWI76_RS18030 at 3747.161 kb on - strand, within BWI76_RS18030 at 3747.163 kb on - strand, within BWI76_RS18030 at 3747.240 kb on - strand at 3747.383 kb on + strand, within BWI76_RS18035 at 3747.643 kb on + strand, within BWI76_RS18035 at 3747.677 kb on + strand, within BWI76_RS18035
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 3,745,277 - -1.2 3,745,295 + -0.7 3,745,324 - -0.2 3,745,367 - -0.3 3,745,369 - +0.8 3,745,487 + +1.9 3,745,523 + -0.2 3,745,549 - -0.2 3,745,554 - +1.5 3,745,634 + +1.7 3,745,642 - +0.0 3,745,711 + +0.7 3,745,722 + -3.6 3,745,727 + -1.5 3,745,727 + -1.4 3,745,727 + +0.1 3,745,727 + +0.2 3,745,735 - -0.3 3,745,735 - +0.6 3,745,735 - -0.3 3,745,735 - -0.2 3,745,735 - -0.9 3,745,738 + +2.4 3,745,741 - -0.3 3,745,746 + -0.4 3,745,746 + -2.2 3,745,746 + +0.8 3,745,746 + -0.1 3,745,746 + +0.6 3,745,746 - -0.2 3,745,754 - +0.0 3,745,754 - +0.1 3,745,767 - -0.3 3,745,767 - -2.0 3,745,777 - +1.3 3,745,786 - -2.2 3,745,791 - -0.0 3,745,791 - +1.1 3,745,791 - +0.3 3,745,793 + +0.2 3,745,801 - -0.7 3,745,811 - +0.6 3,745,811 - -0.7 3,745,815 + -0.3 3,745,815 + +1.2 3,745,815 + -2.5 3,745,815 + +1.2 3,745,823 - +1.0 3,745,823 - -0.4 3,745,823 - +0.9 3,745,823 - -0.4 3,745,903 + -1.0 3,745,984 - +0.1 3,745,989 - -0.3 3,746,062 - -1.0 3,746,062 - -0.1 3,746,093 + -0.2 3,746,101 - +0.8 3,746,147 - +0.4 3,746,159 + -0.1 3,746,159 + -0.4 3,746,159 + +0.0 3,746,179 - -0.1 3,746,179 - -1.2 3,746,181 + -0.5 3,746,181 + +0.1 3,746,181 + -0.2 3,746,181 + +0.8 3,746,181 + -0.6 3,746,181 + -0.5 3,746,181 + -0.8 3,746,181 + +1.0 3,746,181 + +0.1 3,746,181 + -0.7 3,746,181 + -1.5 3,746,181 + +1.2 3,746,189 - -0.5 3,746,189 - -0.3 3,746,189 - -1.8 3,746,189 - -0.3 3,746,189 - +1.1 3,746,189 - -0.7 3,746,189 - -1.8 3,746,240 - BWI76_RS18025 0.16 +0.2 3,746,247 - BWI76_RS18025 0.17 -0.2 3,746,268 + BWI76_RS18025 0.21 -0.8 3,746,268 + BWI76_RS18025 0.21 -0.2 3,746,356 - BWI76_RS18025 0.37 -0.5 3,746,404 + BWI76_RS18025 0.45 -1.8 3,746,475 + BWI76_RS18025 0.58 +0.0 3,746,598 + BWI76_RS18025 0.80 -0.4 3,746,691 + -0.2 3,746,916 + BWI76_RS18030 0.36 -0.3 3,746,928 + BWI76_RS18030 0.39 +0.4 3,746,938 + BWI76_RS18030 0.40 -1.3 3,746,994 + BWI76_RS18030 0.51 -0.3 3,747,017 + BWI76_RS18030 0.56 -1.5 3,747,048 + BWI76_RS18030 0.62 +0.7 3,747,161 - BWI76_RS18030 0.83 +0.0 3,747,163 - BWI76_RS18030 0.84 +0.8 3,747,240 - +0.2 3,747,383 + BWI76_RS18035 0.15 +2.8 3,747,643 + BWI76_RS18035 0.49 -0.7 3,747,677 + BWI76_RS18035 0.54 +0.7
Or see this region's nucleotide sequence