Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16925

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16915 and BWI76_RS16920 are separated by 17 nucleotidesBWI76_RS16920 and BWI76_RS16925 are separated by 1 nucleotidesBWI76_RS16925 and BWI76_RS16930 are separated by 190 nucleotides BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236 _RS16915 BWI76_RS16920: BWI76_RS16920 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 3,508,254 to 3,509,198 _RS16920 BWI76_RS16925: BWI76_RS16925 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,509,200 to 3,510,867 _RS16925 BWI76_RS16930: BWI76_RS16930 - transcriptional regulator, at 3,511,058 to 3,511,864 _RS16930 Position (kb) 3509 3510 3511Strain fitness (log2 ratio) -3 -2 -1 0 1at 3508.418 kb on + strand, within BWI76_RS16920at 3508.568 kb on + strand, within BWI76_RS16920at 3509.124 kb on + strandat 3509.955 kb on - strand, within BWI76_RS16925at 3510.070 kb on - strand, within BWI76_RS16925at 3510.179 kb on - strand, within BWI76_RS16925at 3510.192 kb on + strand, within BWI76_RS16925at 3510.497 kb on - strand, within BWI76_RS16925at 3510.514 kb on - strand, within BWI76_RS16925at 3510.662 kb on - strand, within BWI76_RS16925at 3510.824 kb on - strandat 3510.881 kb on + strandat 3510.966 kb on + strandat 3510.966 kb on + strandat 3511.275 kb on + strand, within BWI76_RS16930at 3511.732 kb on - strand, within BWI76_RS16930at 3511.754 kb on + strand, within BWI76_RS16930

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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3,508,418 + BWI76_RS16920 0.17 +0.6
3,508,568 + BWI76_RS16920 0.33 -0.4
3,509,124 + -0.8
3,509,955 - BWI76_RS16925 0.45 -2.8
3,510,070 - BWI76_RS16925 0.52 -1.2
3,510,179 - BWI76_RS16925 0.59 -0.5
3,510,192 + BWI76_RS16925 0.59 -1.2
3,510,497 - BWI76_RS16925 0.78 +1.4
3,510,514 - BWI76_RS16925 0.79 -0.9
3,510,662 - BWI76_RS16925 0.88 -1.0
3,510,824 - -0.2
3,510,881 + +0.8
3,510,966 + +0.0
3,510,966 + -0.6
3,511,275 + BWI76_RS16930 0.27 -0.0
3,511,732 - BWI76_RS16930 0.84 -0.3
3,511,754 + BWI76_RS16930 0.86 +0.5

Or see this region's nucleotide sequence