Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08000 and BWI76_RS08005 are separated by 186 nucleotides BWI76_RS08005 and BWI76_RS08010 are separated by 137 nucleotides BWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotides
BWI76_RS08000: BWI76_RS08000 - lipoyl synthase, at 1,696,223 to 1,697,188
_RS08000
BWI76_RS08005: BWI76_RS08005 - transcriptional regulator, at 1,697,375 to 1,698,328
_RS08005
BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110
_RS08010
BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465
_RS08015
Position (kb) 1697
1698
1699 Strain fitness (log2 ratio) -1
0
1
2 at 1697.308 kb on - strand at 1697.575 kb on - strand, within BWI76_RS08005 at 1697.970 kb on + strand, within BWI76_RS08005 at 1698.083 kb on + strand, within BWI76_RS08005 at 1698.091 kb on - strand, within BWI76_RS08005 at 1698.140 kb on + strand, within BWI76_RS08005 at 1698.211 kb on - strand, within BWI76_RS08005 at 1698.357 kb on + strand at 1698.357 kb on - strand at 1698.365 kb on - strand at 1698.459 kb on - strand at 1699.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 1,697,308 - -0.6 1,697,575 - BWI76_RS08005 0.21 +1.9 1,697,970 + BWI76_RS08005 0.62 +1.4 1,698,083 + BWI76_RS08005 0.74 -0.1 1,698,091 - BWI76_RS08005 0.75 +0.8 1,698,140 + BWI76_RS08005 0.80 -0.6 1,698,211 - BWI76_RS08005 0.88 +1.5 1,698,357 + +1.1 1,698,357 - -0.3 1,698,365 - +0.4 1,698,459 - +0.0 1,699,151 + -0.3
Or see this region's nucleotide sequence