Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07485 and BWI76_RS07490 overlap by 72 nucleotides BWI76_RS07490 and BWI76_RS07495 are separated by 492 nucleotides BWI76_RS07495 and BWI76_RS07500 are separated by 69 nucleotides
BWI76_RS07485: BWI76_RS07485 - esterase, at 1,579,511 to 1,581,133
_RS07485
BWI76_RS07490: BWI76_RS07490 - hypothetical protein, at 1,581,062 to 1,581,310
_RS07490
BWI76_RS07495: BWI76_RS07495 - carbonate dehydratase, at 1,581,803 to 1,584,526
_RS07495
BWI76_RS07500: BWI76_RS07500 - RND transporter MFP subunit, at 1,584,596 to 1,585,675
_RS07500
Position (kb)
1581
1582
1583
1584
1585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1580.970 kb on + strand, within BWI76_RS07485 at 1580.978 kb on - strand at 1580.978 kb on - strand at 1580.982 kb on + strand at 1580.992 kb on - strand at 1581.007 kb on + strand at 1581.015 kb on - strand at 1581.015 kb on - strand at 1581.029 kb on + strand at 1581.154 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.156 kb on + strand, within BWI76_RS07490 at 1581.164 kb on - strand, within BWI76_RS07490 at 1581.164 kb on - strand, within BWI76_RS07490 at 1581.174 kb on - strand, within BWI76_RS07490 at 1581.184 kb on - strand, within BWI76_RS07490 at 1581.211 kb on + strand, within BWI76_RS07490 at 1581.213 kb on + strand, within BWI76_RS07490 at 1581.219 kb on - strand, within BWI76_RS07490 at 1581.225 kb on - strand, within BWI76_RS07490 at 1581.254 kb on - strand, within BWI76_RS07490 at 1581.259 kb on - strand, within BWI76_RS07490 at 1581.279 kb on + strand, within BWI76_RS07490 at 1581.279 kb on + strand, within BWI76_RS07490 at 1581.281 kb on + strand, within BWI76_RS07490 at 1581.287 kb on - strand at 1581.287 kb on - strand at 1581.287 kb on - strand at 1581.353 kb on - strand at 1581.361 kb on + strand at 1581.361 kb on + strand at 1581.369 kb on - strand at 1581.393 kb on + strand at 1581.503 kb on - strand at 1581.527 kb on - strand at 1581.527 kb on - strand at 1581.535 kb on - strand at 1581.565 kb on + strand at 1581.566 kb on + strand at 1581.574 kb on + strand at 1581.574 kb on + strand at 1581.575 kb on - strand at 1581.577 kb on - strand at 1581.578 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.582 kb on - strand at 1581.597 kb on + strand at 1581.605 kb on - strand at 1581.605 kb on - strand at 1581.615 kb on - strand at 1581.615 kb on - strand at 1581.730 kb on + strand at 1581.750 kb on + strand at 1581.755 kb on + strand at 1581.759 kb on + strand at 1581.768 kb on - strand at 1581.795 kb on + strand at 1581.800 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.837 kb on + strand at 1581.845 kb on - strand at 1581.851 kb on + strand at 1581.851 kb on + strand at 1581.859 kb on - strand at 1581.859 kb on - strand at 1581.861 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.864 kb on + strand at 1581.866 kb on + strand at 1581.866 kb on + strand at 1581.869 kb on - strand at 1581.872 kb on - strand at 1581.872 kb on - strand at 1581.872 kb on - strand at 1581.874 kb on - strand at 1581.874 kb on - strand at 1581.876 kb on + strand at 1581.876 kb on + strand at 1581.879 kb on - strand at 1581.884 kb on - strand at 1581.884 kb on - strand at 1581.885 kb on - strand at 1581.887 kb on + strand at 1581.895 kb on - strand at 1581.895 kb on - strand at 1581.898 kb on + strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.906 kb on - strand at 1581.908 kb on - strand at 1581.935 kb on + strand at 1582.018 kb on + strand at 1582.253 kb on - strand, within BWI76_RS07495 at 1582.253 kb on - strand, within BWI76_RS07495 at 1582.908 kb on - strand, within BWI76_RS07495 at 1582.914 kb on + strand, within BWI76_RS07495 at 1582.928 kb on + strand, within BWI76_RS07495 at 1583.314 kb on - strand, within BWI76_RS07495 at 1583.346 kb on - strand, within BWI76_RS07495 at 1583.384 kb on + strand, within BWI76_RS07495 at 1583.675 kb on - strand, within BWI76_RS07495 at 1583.697 kb on - strand, within BWI76_RS07495 at 1584.078 kb on - strand, within BWI76_RS07495 at 1584.085 kb on - strand, within BWI76_RS07495 at 1584.258 kb on + strand at 1584.258 kb on - strand at 1584.309 kb on + strand at 1584.309 kb on + strand at 1584.317 kb on - strand at 1584.674 kb on + strand at 1584.760 kb on - strand, within BWI76_RS07500 at 1584.862 kb on + strand, within BWI76_RS07500 at 1584.862 kb on + strand, within BWI76_RS07500 at 1584.901 kb on - strand, within BWI76_RS07500 at 1585.379 kb on + strand, within BWI76_RS07500 at 1585.525 kb on + strand, within BWI76_RS07500
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 1,580,970 + BWI76_RS07485 0.90 +2.3 1,580,978 - -3.0 1,580,978 - +0.0 1,580,982 + +0.2 1,580,992 - -0.4 1,581,007 + +0.3 1,581,015 - -0.8 1,581,015 - -1.8 1,581,029 + -0.1 1,581,154 + BWI76_RS07490 0.37 -0.1 1,581,156 + BWI76_RS07490 0.38 -0.2 1,581,156 + BWI76_RS07490 0.38 +1.6 1,581,156 + BWI76_RS07490 0.38 +1.0 1,581,164 - BWI76_RS07490 0.41 +0.9 1,581,164 - BWI76_RS07490 0.41 -2.0 1,581,174 - BWI76_RS07490 0.45 -0.1 1,581,184 - BWI76_RS07490 0.49 +2.4 1,581,211 + BWI76_RS07490 0.60 -1.5 1,581,213 + BWI76_RS07490 0.61 -0.6 1,581,219 - BWI76_RS07490 0.63 -0.5 1,581,225 - BWI76_RS07490 0.65 +0.1 1,581,254 - BWI76_RS07490 0.77 -0.4 1,581,259 - BWI76_RS07490 0.79 -0.8 1,581,279 + BWI76_RS07490 0.87 +0.1 1,581,279 + BWI76_RS07490 0.87 -0.6 1,581,281 + BWI76_RS07490 0.88 -0.1 1,581,287 - -1.3 1,581,287 - -1.8 1,581,287 - +0.5 1,581,353 - +1.2 1,581,361 + -0.6 1,581,361 + +0.1 1,581,369 - -0.7 1,581,393 + -0.2 1,581,503 - +2.6 1,581,527 - +1.0 1,581,527 - -0.1 1,581,535 - -0.6 1,581,565 + -1.7 1,581,566 + -0.1 1,581,574 + -0.1 1,581,574 + -1.3 1,581,575 - -1.6 1,581,577 - +1.5 1,581,578 - -2.0 1,581,582 - +0.6 1,581,582 - -0.0 1,581,582 - -0.4 1,581,582 - -0.0 1,581,582 - +0.9 1,581,582 - +1.2 1,581,582 - +1.4 1,581,597 + -0.1 1,581,605 - +0.4 1,581,605 - -0.0 1,581,615 - +1.2 1,581,615 - -0.5 1,581,730 + +0.1 1,581,750 + +0.3 1,581,755 + -0.0 1,581,759 + +0.3 1,581,768 - -1.5 1,581,795 + +1.4 1,581,800 + -0.7 1,581,837 + +0.6 1,581,837 + -1.7 1,581,837 + +0.6 1,581,837 + -0.7 1,581,845 - -0.7 1,581,851 + -0.7 1,581,851 + +1.9 1,581,859 - +0.0 1,581,859 - +0.8 1,581,861 + -1.5 1,581,864 + +1.4 1,581,864 + +0.5 1,581,864 + +0.1 1,581,864 + -0.5 1,581,864 + -0.9 1,581,866 + +0.4 1,581,866 + -1.5 1,581,869 - +0.7 1,581,872 - -1.1 1,581,872 - +0.1 1,581,872 - +0.1 1,581,874 - -0.4 1,581,874 - +0.0 1,581,876 + +0.6 1,581,876 + -0.4 1,581,879 - -0.8 1,581,884 - +0.1 1,581,884 - -0.5 1,581,885 - -0.5 1,581,887 + -1.5 1,581,895 - -0.5 1,581,895 - +0.1 1,581,898 + -0.1 1,581,906 - +0.9 1,581,906 - +0.5 1,581,906 - +0.6 1,581,906 - -0.2 1,581,906 - -0.6 1,581,908 - +0.3 1,581,935 + -0.6 1,582,018 + +0.7 1,582,253 - BWI76_RS07495 0.17 -1.2 1,582,253 - BWI76_RS07495 0.17 -0.7 1,582,908 - BWI76_RS07495 0.41 +3.2 1,582,914 + BWI76_RS07495 0.41 -0.0 1,582,928 + BWI76_RS07495 0.41 +0.0 1,583,314 - BWI76_RS07495 0.55 -0.3 1,583,346 - BWI76_RS07495 0.57 -1.7 1,583,384 + BWI76_RS07495 0.58 +1.0 1,583,675 - BWI76_RS07495 0.69 -1.1 1,583,697 - BWI76_RS07495 0.70 -0.8 1,584,078 - BWI76_RS07495 0.84 -0.5 1,584,085 - BWI76_RS07495 0.84 -0.5 1,584,258 + +1.1 1,584,258 - +1.1 1,584,309 + +1.0 1,584,309 + -1.0 1,584,317 - +0.9 1,584,674 + +2.4 1,584,760 - BWI76_RS07500 0.15 +0.3 1,584,862 + BWI76_RS07500 0.25 -0.3 1,584,862 + BWI76_RS07500 0.25 +1.1 1,584,901 - BWI76_RS07500 0.28 -3.0 1,585,379 + BWI76_RS07500 0.72 +0.8 1,585,525 + BWI76_RS07500 0.86 +0.7
Or see this region's nucleotide sequence