Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01685 and BWI76_RS01690 are separated by 185 nucleotides BWI76_RS01690 and BWI76_RS01695 are separated by 261 nucleotides
BWI76_RS01685: BWI76_RS01685 - methionine synthase, at 343,151 to 346,834
_RS01685
BWI76_RS01690: BWI76_RS01690 - membrane protein, at 347,020 to 348,651
_RS01690
BWI76_RS01695: BWI76_RS01695 - IS110 family transposase, at 348,913 to 349,893
_RS01695
Position (kb) 347
348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 346.086 kb on - strand, within BWI76_RS01685 at 346.116 kb on - strand, within BWI76_RS01685 at 346.329 kb on + strand, within BWI76_RS01685 at 346.413 kb on - strand, within BWI76_RS01685 at 346.575 kb on + strand at 346.610 kb on - strand at 346.756 kb on - strand at 346.872 kb on + strand at 346.909 kb on + strand at 346.909 kb on + strand at 346.910 kb on - strand at 346.983 kb on - strand at 347.029 kb on + strand at 347.043 kb on - strand at 347.097 kb on - strand at 347.097 kb on - strand at 347.121 kb on - strand at 347.263 kb on + strand, within BWI76_RS01690 at 347.321 kb on + strand, within BWI76_RS01690 at 347.478 kb on - strand, within BWI76_RS01690 at 347.495 kb on - strand, within BWI76_RS01690 at 347.657 kb on - strand, within BWI76_RS01690 at 347.813 kb on - strand, within BWI76_RS01690 at 347.927 kb on - strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 347.994 kb on + strand, within BWI76_RS01690 at 348.590 kb on + strand at 348.598 kb on - strand at 348.607 kb on - strand at 348.669 kb on + strand at 348.971 kb on + strand at 349.010 kb on + strand at 349.027 kb on + strand, within BWI76_RS01695 at 349.073 kb on + strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.081 kb on - strand, within BWI76_RS01695 at 349.093 kb on + strand, within BWI76_RS01695 at 349.097 kb on + strand, within BWI76_RS01695 at 349.102 kb on + strand, within BWI76_RS01695 at 349.492 kb on - strand, within BWI76_RS01695 at 349.569 kb on + strand, within BWI76_RS01695 at 349.607 kb on + strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 346,086 - BWI76_RS01685 0.80 -1.4 346,116 - BWI76_RS01685 0.80 -1.5 346,329 + BWI76_RS01685 0.86 -0.8 346,413 - BWI76_RS01685 0.89 +0.1 346,575 + -0.0 346,610 - -0.5 346,756 - -0.3 346,872 + -0.2 346,909 + +1.8 346,909 + +0.9 346,910 - +0.1 346,983 - +0.8 347,029 + +0.7 347,043 - +0.8 347,097 - -0.6 347,097 - +0.5 347,121 - -1.1 347,263 + BWI76_RS01690 0.15 -0.0 347,321 + BWI76_RS01690 0.18 -0.5 347,478 - BWI76_RS01690 0.28 +0.8 347,495 - BWI76_RS01690 0.29 -0.2 347,657 - BWI76_RS01690 0.39 -0.1 347,813 - BWI76_RS01690 0.49 -0.2 347,927 - BWI76_RS01690 0.56 +1.5 347,994 + BWI76_RS01690 0.60 -0.7 347,994 + BWI76_RS01690 0.60 +2.0 348,590 + -0.6 348,598 - -1.0 348,607 - -1.2 348,669 + +1.3 348,971 + +0.9 349,010 + +0.7 349,027 + BWI76_RS01695 0.12 -1.1 349,073 + BWI76_RS01695 0.16 -0.3 349,081 - BWI76_RS01695 0.17 +0.0 349,081 - BWI76_RS01695 0.17 +0.3 349,081 - BWI76_RS01695 0.17 -0.2 349,081 - BWI76_RS01695 0.17 -2.1 349,093 + BWI76_RS01695 0.18 -0.5 349,097 + BWI76_RS01695 0.19 +0.3 349,102 + BWI76_RS01695 0.19 -0.8 349,492 - BWI76_RS01695 0.59 -0.3 349,569 + BWI76_RS01695 0.67 -2.8 349,607 + BWI76_RS01695 0.71 +0.3 349,633 - BWI76_RS01695 0.73 -0.3 349,633 - BWI76_RS01695 0.73 -0.7
Or see this region's nucleotide sequence