Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00605

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00600 and BWI76_RS00605 are separated by 114 nucleotidesBWI76_RS00605 and BWI76_RS00610 are separated by 41 nucleotides BWI76_RS00600: BWI76_RS00600 - LacI family transcriptional regulator, at 119,728 to 120,723 _RS00600 BWI76_RS00605: BWI76_RS00605 - alpha-galactosidase, at 120,838 to 122,961 _RS00605 BWI76_RS00610: BWI76_RS00610 - MFS transporter, at 123,003 to 124,268 _RS00610 Position (kb) 120 121 122 123Strain fitness (log2 ratio) -2 -1 0 1 2at 119.850 kb on - strand, within BWI76_RS00600at 119.890 kb on - strand, within BWI76_RS00600at 120.058 kb on - strand, within BWI76_RS00600at 120.072 kb on - strand, within BWI76_RS00600at 120.072 kb on - strand, within BWI76_RS00600at 120.118 kb on - strand, within BWI76_RS00600at 120.418 kb on - strand, within BWI76_RS00600at 120.613 kb on + strand, within BWI76_RS00600at 121.130 kb on + strand, within BWI76_RS00605at 121.201 kb on + strand, within BWI76_RS00605at 121.222 kb on + strand, within BWI76_RS00605at 121.230 kb on - strand, within BWI76_RS00605at 121.284 kb on + strand, within BWI76_RS00605at 121.458 kb on + strand, within BWI76_RS00605at 121.644 kb on - strand, within BWI76_RS00605at 121.654 kb on - strand, within BWI76_RS00605at 121.720 kb on + strand, within BWI76_RS00605at 121.765 kb on - strand, within BWI76_RS00605at 121.832 kb on + strand, within BWI76_RS00605at 121.913 kb on + strand, within BWI76_RS00605at 121.990 kb on + strand, within BWI76_RS00605at 121.998 kb on - strand, within BWI76_RS00605at 121.998 kb on - strand, within BWI76_RS00605at 122.033 kb on + strand, within BWI76_RS00605at 122.147 kb on + strand, within BWI76_RS00605at 122.179 kb on - strandat 122.193 kb on + strand, within BWI76_RS00605at 122.288 kb on - strand, within BWI76_RS00605at 122.317 kb on + strand, within BWI76_RS00605at 122.337 kb on + strand, within BWI76_RS00605at 122.378 kb on + strand, within BWI76_RS00605at 122.477 kb on - strand, within BWI76_RS00605at 122.861 kb on - strandat 122.986 kb on + strandat 123.057 kb on - strandat 123.127 kb on + strandat 123.135 kb on - strand, within BWI76_RS00610at 123.162 kb on - strand, within BWI76_RS00610at 123.204 kb on - strand, within BWI76_RS00610at 123.231 kb on - strand, within BWI76_RS00610at 123.500 kb on + strand, within BWI76_RS00610at 123.500 kb on + strand, within BWI76_RS00610at 123.505 kb on + strand, within BWI76_RS00610at 123.508 kb on - strand, within BWI76_RS00610at 123.590 kb on - strand, within BWI76_RS00610at 123.598 kb on + strand, within BWI76_RS00610at 123.598 kb on - strand, within BWI76_RS00610at 123.625 kb on - strand, within BWI76_RS00610at 123.796 kb on + strand, within BWI76_RS00610at 123.855 kb on + strand, within BWI76_RS00610

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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119,850 - BWI76_RS00600 0.12 +2.6
119,890 - BWI76_RS00600 0.16 +0.8
120,058 - BWI76_RS00600 0.33 +0.4
120,072 - BWI76_RS00600 0.35 -2.4
120,072 - BWI76_RS00600 0.35 -0.5
120,118 - BWI76_RS00600 0.39 -0.3
120,418 - BWI76_RS00600 0.69 +0.3
120,613 + BWI76_RS00600 0.89 +0.8
121,130 + BWI76_RS00605 0.14 -0.9
121,201 + BWI76_RS00605 0.17 -2.2
121,222 + BWI76_RS00605 0.18 -0.6
121,230 - BWI76_RS00605 0.18 -1.0
121,284 + BWI76_RS00605 0.21 -1.7
121,458 + BWI76_RS00605 0.29 -0.3
121,644 - BWI76_RS00605 0.38 -0.4
121,654 - BWI76_RS00605 0.38 +0.0
121,720 + BWI76_RS00605 0.42 -0.2
121,765 - BWI76_RS00605 0.44 -2.6
121,832 + BWI76_RS00605 0.47 +0.8
121,913 + BWI76_RS00605 0.51 -0.2
121,990 + BWI76_RS00605 0.54 -0.1
121,998 - BWI76_RS00605 0.55 -1.5
121,998 - BWI76_RS00605 0.55 -0.4
122,033 + BWI76_RS00605 0.56 -0.6
122,147 + BWI76_RS00605 0.62 -0.5
122,179 - +2.0
122,193 + BWI76_RS00605 0.64 +0.7
122,288 - BWI76_RS00605 0.68 -1.3
122,317 + BWI76_RS00605 0.70 -2.2
122,337 + BWI76_RS00605 0.71 -0.2
122,378 + BWI76_RS00605 0.73 +0.2
122,477 - BWI76_RS00605 0.77 -0.5
122,861 - -0.6
122,986 + -0.9
123,057 - +0.0
123,127 + +0.1
123,135 - BWI76_RS00610 0.10 +0.0
123,162 - BWI76_RS00610 0.13 -1.6
123,204 - BWI76_RS00610 0.16 -1.2
123,231 - BWI76_RS00610 0.18 +2.3
123,500 + BWI76_RS00610 0.39 +0.6
123,500 + BWI76_RS00610 0.39 -1.1
123,505 + BWI76_RS00610 0.40 -0.4
123,508 - BWI76_RS00610 0.40 -1.7
123,590 - BWI76_RS00610 0.46 -0.6
123,598 + BWI76_RS00610 0.47 -0.0
123,598 - BWI76_RS00610 0.47 +1.6
123,625 - BWI76_RS00610 0.49 -0.0
123,796 + BWI76_RS00610 0.63 +0.3
123,855 + BWI76_RS00610 0.67 +0.3

Or see this region's nucleotide sequence