Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27520

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27515 and BWI76_RS27520 are separated by 99 nucleotidesBWI76_RS27520 and BWI76_RS27525 are separated by 287 nucleotides BWI76_RS27515: BWI76_RS27515 - glycerol kinase, at 5,708,315 to 5,709,826 _RS27515 BWI76_RS27520: BWI76_RS27520 - sodium/glutamate symporter, at 5,709,926 to 5,711,128 _RS27520 BWI76_RS27525: BWI76_RS27525 - xanthine permease XanP, at 5,711,416 to 5,712,807 _RS27525 Position (kb) 5709 5710 5711 5712Strain fitness (log2 ratio) -2 -1 0 1 2at 5708.984 kb on + strand, within BWI76_RS27515at 5708.995 kb on + strand, within BWI76_RS27515at 5709.071 kb on + strand, within BWI76_RS27515at 5709.084 kb on - strand, within BWI76_RS27515at 5709.131 kb on - strand, within BWI76_RS27515at 5709.173 kb on + strand, within BWI76_RS27515at 5709.532 kb on - strand, within BWI76_RS27515at 5709.612 kb on + strand, within BWI76_RS27515at 5709.648 kb on - strand, within BWI76_RS27515at 5709.684 kb on + strandat 5709.904 kb on - strandat 5709.956 kb on - strandat 5710.055 kb on - strand, within BWI76_RS27520at 5710.100 kb on + strand, within BWI76_RS27520at 5710.141 kb on - strand, within BWI76_RS27520at 5710.163 kb on - strand, within BWI76_RS27520at 5710.266 kb on + strand, within BWI76_RS27520at 5710.443 kb on - strand, within BWI76_RS27520at 5710.891 kb on + strand, within BWI76_RS27520at 5711.140 kb on + strandat 5711.399 kb on + strandat 5711.513 kb on + strandat 5711.541 kb on - strandat 5711.593 kb on + strand, within BWI76_RS27525at 5711.601 kb on - strand, within BWI76_RS27525at 5711.601 kb on - strand, within BWI76_RS27525at 5711.601 kb on - strand, within BWI76_RS27525at 5711.696 kb on + strand, within BWI76_RS27525at 5711.706 kb on + strand, within BWI76_RS27525at 5711.734 kb on - strand, within BWI76_RS27525at 5711.756 kb on - strand, within BWI76_RS27525at 5711.780 kb on + strand, within BWI76_RS27525at 5711.819 kb on - strand, within BWI76_RS27525at 5711.837 kb on + strand, within BWI76_RS27525at 5711.842 kb on - strand, within BWI76_RS27525at 5711.842 kb on - strand, within BWI76_RS27525at 5711.845 kb on - strand, within BWI76_RS27525at 5711.848 kb on + strand, within BWI76_RS27525at 5711.888 kb on - strand, within BWI76_RS27525at 5711.908 kb on + strand, within BWI76_RS27525at 5711.952 kb on - strand, within BWI76_RS27525at 5711.990 kb on + strand, within BWI76_RS27525at 5712.073 kb on - strand, within BWI76_RS27525

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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5,708,984 + BWI76_RS27515 0.44 -2.0
5,708,995 + BWI76_RS27515 0.45 +0.0
5,709,071 + BWI76_RS27515 0.50 -0.1
5,709,084 - BWI76_RS27515 0.51 -2.4
5,709,131 - BWI76_RS27515 0.54 -0.3
5,709,173 + BWI76_RS27515 0.57 +0.1
5,709,532 - BWI76_RS27515 0.80 -1.0
5,709,612 + BWI76_RS27515 0.86 -0.5
5,709,648 - BWI76_RS27515 0.88 -1.5
5,709,684 + +0.5
5,709,904 - -0.5
5,709,956 - +0.2
5,710,055 - BWI76_RS27520 0.11 -0.5
5,710,100 + BWI76_RS27520 0.14 +0.0
5,710,141 - BWI76_RS27520 0.18 -1.1
5,710,163 - BWI76_RS27520 0.20 -2.7
5,710,266 + BWI76_RS27520 0.28 -0.9
5,710,443 - BWI76_RS27520 0.43 -1.9
5,710,891 + BWI76_RS27520 0.80 -0.3
5,711,140 + +0.5
5,711,399 + -0.9
5,711,513 + -0.6
5,711,541 - +0.6
5,711,593 + BWI76_RS27525 0.13 -1.9
5,711,601 - BWI76_RS27525 0.13 -0.5
5,711,601 - BWI76_RS27525 0.13 +1.1
5,711,601 - BWI76_RS27525 0.13 -0.4
5,711,696 + BWI76_RS27525 0.20 +0.1
5,711,706 + BWI76_RS27525 0.21 -2.1
5,711,734 - BWI76_RS27525 0.23 -0.1
5,711,756 - BWI76_RS27525 0.24 -0.4
5,711,780 + BWI76_RS27525 0.26 -1.4
5,711,819 - BWI76_RS27525 0.29 -0.3
5,711,837 + BWI76_RS27525 0.30 -1.3
5,711,842 - BWI76_RS27525 0.31 -0.0
5,711,842 - BWI76_RS27525 0.31 -0.5
5,711,845 - BWI76_RS27525 0.31 +1.2
5,711,848 + BWI76_RS27525 0.31 -1.1
5,711,888 - BWI76_RS27525 0.34 -0.7
5,711,908 + BWI76_RS27525 0.35 -1.8
5,711,952 - BWI76_RS27525 0.39 +1.8
5,711,990 + BWI76_RS27525 0.41 +2.1
5,712,073 - BWI76_RS27525 0.47 -0.1

Or see this region's nucleotide sequence