Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26800 and BWI76_RS26805 overlap by 4 nucleotides BWI76_RS26805 and BWI76_RS26810 overlap by 4 nucleotides BWI76_RS26810 and BWI76_RS26815 are separated by 83 nucleotides BWI76_RS26815 and BWI76_RS26820 are separated by 351 nucleotides BWI76_RS26820 and BWI76_RS26825 overlap by 20 nucleotides BWI76_RS26825 and BWI76_RS26830 are separated by 48 nucleotides
BWI76_RS26800: BWI76_RS26800 - cellulose biosynthesis protein BcsE, at 5,557,019 to 5,558,575
_RS26800
BWI76_RS26805: BWI76_RS26805 - cellulose biosynthesis protein BcsF, at 5,558,572 to 5,558,769
_RS26805
BWI76_RS26810: BWI76_RS26810 - cellulose biosynthesis protein BcsG, at 5,558,766 to 5,560,442
_RS26810
BWI76_RS26815: BWI76_RS26815 - small toxic polypeptide ldrD (Modular protein), at 5,560,526 to 5,560,651
_RS26815
BWI76_RS26820: BWI76_RS26820 - small toxic polypeptide ldrD (Modular protein), at 5,561,003 to 5,561,128
_RS26820
BWI76_RS26825: BWI76_RS26825 - hypothetical protein, at 5,561,109 to 5,561,327
_RS26825
BWI76_RS26830: BWI76_RS26830 - endoglucanase, at 5,561,376 to 5,562,380
_RS26830
Position (kb) 5558
5559
5560
5561 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 5557.993 kb on - strand, within BWI76_RS26800 at 5558.006 kb on - strand, within BWI76_RS26800 at 5558.006 kb on - strand, within BWI76_RS26800 at 5558.006 kb on - strand, within BWI76_RS26800 at 5558.222 kb on - strand, within BWI76_RS26800 at 5558.226 kb on + strand at 5558.236 kb on + strand, within BWI76_RS26800 at 5558.244 kb on - strand, within BWI76_RS26800 at 5558.244 kb on - strand, within BWI76_RS26800 at 5558.244 kb on - strand, within BWI76_RS26800 at 5558.244 kb on - strand, within BWI76_RS26800 at 5558.244 kb on - strand, within BWI76_RS26800 at 5558.293 kb on + strand, within BWI76_RS26800 at 5558.318 kb on + strand, within BWI76_RS26800 at 5558.326 kb on - strand, within BWI76_RS26800 at 5558.326 kb on - strand, within BWI76_RS26800 at 5558.556 kb on - strand at 5558.610 kb on - strand, within BWI76_RS26805 at 5558.625 kb on - strand, within BWI76_RS26805 at 5558.675 kb on + strand, within BWI76_RS26805 at 5558.727 kb on + strand, within BWI76_RS26805 at 5558.869 kb on + strand at 5558.942 kb on + strand, within BWI76_RS26810 at 5558.942 kb on + strand, within BWI76_RS26810 at 5558.986 kb on + strand, within BWI76_RS26810 at 5558.986 kb on + strand, within BWI76_RS26810 at 5558.986 kb on + strand, within BWI76_RS26810 at 5558.994 kb on - strand, within BWI76_RS26810 at 5559.397 kb on + strand, within BWI76_RS26810 at 5559.405 kb on - strand, within BWI76_RS26810 at 5559.454 kb on + strand, within BWI76_RS26810 at 5559.497 kb on + strand, within BWI76_RS26810 at 5559.722 kb on + strand, within BWI76_RS26810 at 5559.781 kb on + strand, within BWI76_RS26810 at 5559.789 kb on - strand, within BWI76_RS26810 at 5559.789 kb on - strand, within BWI76_RS26810 at 5559.878 kb on - strand, within BWI76_RS26810 at 5559.964 kb on + strand at 5559.977 kb on - strand, within BWI76_RS26810 at 5559.982 kb on - strand, within BWI76_RS26810 at 5560.369 kb on - strand at 5560.399 kb on + strand at 5560.492 kb on - strand at 5560.729 kb on - strand at 5560.964 kb on - strand at 5561.144 kb on + strand, within BWI76_RS26825 at 5561.342 kb on + strand at 5561.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 5,557,993 - BWI76_RS26800 0.63 +5.1 5,558,006 - BWI76_RS26800 0.63 +2.3 5,558,006 - BWI76_RS26800 0.63 +3.7 5,558,006 - BWI76_RS26800 0.63 +4.5 5,558,222 - BWI76_RS26800 0.77 +3.8 5,558,226 + +2.3 5,558,236 + BWI76_RS26800 0.78 +0.5 5,558,244 - BWI76_RS26800 0.79 +5.0 5,558,244 - BWI76_RS26800 0.79 +3.4 5,558,244 - BWI76_RS26800 0.79 +3.2 5,558,244 - BWI76_RS26800 0.79 +3.9 5,558,244 - BWI76_RS26800 0.79 +5.6 5,558,293 + BWI76_RS26800 0.82 +1.5 5,558,318 + BWI76_RS26800 0.83 +0.3 5,558,326 - BWI76_RS26800 0.84 +4.1 5,558,326 - BWI76_RS26800 0.84 +5.4 5,558,556 - +7.2 5,558,610 - BWI76_RS26805 0.19 +5.0 5,558,625 - BWI76_RS26805 0.27 +4.2 5,558,675 + BWI76_RS26805 0.52 +3.0 5,558,727 + BWI76_RS26805 0.78 -0.7 5,558,869 + +4.3 5,558,942 + BWI76_RS26810 0.10 +4.1 5,558,942 + BWI76_RS26810 0.10 +3.5 5,558,986 + BWI76_RS26810 0.13 +3.0 5,558,986 + BWI76_RS26810 0.13 +3.6 5,558,986 + BWI76_RS26810 0.13 +4.1 5,558,994 - BWI76_RS26810 0.14 +4.1 5,559,397 + BWI76_RS26810 0.38 +4.1 5,559,405 - BWI76_RS26810 0.38 +1.1 5,559,454 + BWI76_RS26810 0.41 +2.9 5,559,497 + BWI76_RS26810 0.44 +3.7 5,559,722 + BWI76_RS26810 0.57 +4.0 5,559,781 + BWI76_RS26810 0.61 +4.7 5,559,789 - BWI76_RS26810 0.61 +5.4 5,559,789 - BWI76_RS26810 0.61 +4.1 5,559,878 - BWI76_RS26810 0.66 +3.8 5,559,964 + +2.5 5,559,977 - BWI76_RS26810 0.72 +3.1 5,559,982 - BWI76_RS26810 0.73 +4.1 5,560,369 - +4.0 5,560,399 + +2.4 5,560,492 - -0.0 5,560,729 - +0.4 5,560,964 - +1.7 5,561,144 + BWI76_RS26825 0.16 -0.3 5,561,342 + +2.3 5,561,398 + -0.6
Or see this region's nucleotide sequence