Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25485

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25480 and BWI76_RS25485 are separated by 2 nucleotidesBWI76_RS25485 and BWI76_RS25490 are separated by 7 nucleotidesBWI76_RS25490 and BWI76_RS25495 overlap by 1 nucleotides BWI76_RS25480: BWI76_RS25480 - ribonuclease E/G, at 5,296,898 to 5,298,367 _RS25480 BWI76_RS25485: BWI76_RS25485 - septum formation protein Maf, at 5,298,370 to 5,298,951 _RS25485 BWI76_RS25490: BWI76_RS25490 - rod shape-determining protein MreD, at 5,298,959 to 5,299,447 _RS25490 BWI76_RS25495: BWI76_RS25495 - rod shape-determining protein MreC, at 5,299,447 to 5,300,433 _RS25495 Position (kb) 5298 5299Strain fitness (log2 ratio) -2 -1 0 1 2at 5297.525 kb on - strand, within BWI76_RS25480at 5297.591 kb on - strand, within BWI76_RS25480at 5297.593 kb on + strand, within BWI76_RS25480at 5297.654 kb on + strand, within BWI76_RS25480at 5297.865 kb on + strand, within BWI76_RS25480at 5298.411 kb on + strandat 5298.870 kb on - strand, within BWI76_RS25485

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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5,297,525 - BWI76_RS25480 0.43 +0.7
5,297,591 - BWI76_RS25480 0.47 -1.9
5,297,593 + BWI76_RS25480 0.47 +1.4
5,297,654 + BWI76_RS25480 0.51 -0.8
5,297,865 + BWI76_RS25480 0.66 +2.4
5,298,411 + +0.2
5,298,870 - BWI76_RS25485 0.86 +1.7

Or see this region's nucleotide sequence