Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25370 and BWI76_RS25375 are separated by 26 nucleotides BWI76_RS25375 and BWI76_RS25380 are separated by 126 nucleotides BWI76_RS25380 and BWI76_RS25385 are separated by 15 nucleotides
BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441
_RS25370
BWI76_RS25375: BWI76_RS25375 - hypothetical protein, at 5,274,468 to 5,275,166
_RS25375
BWI76_RS25380: BWI76_RS25380 - oxaloacetate decarboxylase subunit beta, at 5,275,293 to 5,276,594
_RS25380
BWI76_RS25385: BWI76_RS25385 - oxaloacetate decarboxylase subunit alpha, at 5,276,610 to 5,278,376
_RS25385
Position (kb) 5275
5276
5277 Strain fitness (log2 ratio) -1
0
1
2 at 5274.451 kb on + strand at 5274.490 kb on + strand at 5274.574 kb on - strand, within BWI76_RS25375 at 5274.606 kb on + strand at 5274.825 kb on - strand, within BWI76_RS25375 at 5275.217 kb on + strand at 5275.293 kb on - strand at 5275.946 kb on - strand, within BWI76_RS25380 at 5276.558 kb on + strand at 5276.622 kb on + strand at 5276.924 kb on - strand, within BWI76_RS25385 at 5277.076 kb on - strand, within BWI76_RS25385 at 5277.088 kb on - strand, within BWI76_RS25385
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 5,274,451 + +0.1 5,274,490 + -0.1 5,274,574 - BWI76_RS25375 0.15 -0.3 5,274,606 + +2.5 5,274,825 - BWI76_RS25375 0.51 +0.4 5,275,217 + +0.9 5,275,293 - -0.4 5,275,946 - BWI76_RS25380 0.50 -1.0 5,276,558 + +0.0 5,276,622 + -0.5 5,276,924 - BWI76_RS25385 0.18 -0.3 5,277,076 - BWI76_RS25385 0.26 +1.4 5,277,088 - BWI76_RS25385 0.27 -0.1
Or see this region's nucleotide sequence