Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22220 and BWI76_RS22225 are separated by 13 nucleotides BWI76_RS22225 and BWI76_RS22230 are separated by 17 nucleotides BWI76_RS22230 and BWI76_RS22235 are separated by 133 nucleotides BWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotides
BWI76_RS22220: BWI76_RS22220 - PTS glucitol/sorbitol transporter subunit IIB, at 4,632,684 to 4,633,661
_RS22220
BWI76_RS22225: BWI76_RS22225 - PTS sorbitol transporter subunit IIA, at 4,633,675 to 4,634,037
_RS22225
BWI76_RS22230: BWI76_RS22230 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (from data) , at 4,634,055 to 4,634,834
_RS22230
BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327
_RS22235
BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169
_RS22240
Position (kb)
4634
4635 Strain fitness (log2 ratio)
-1
0
1 at 4633.055 kb on + strand, within BWI76_RS22220 at 4633.335 kb on - strand, within BWI76_RS22220 at 4633.346 kb on - strand, within BWI76_RS22220 at 4633.415 kb on + strand, within BWI76_RS22220 at 4633.551 kb on + strand, within BWI76_RS22220 at 4633.559 kb on - strand, within BWI76_RS22220 at 4633.559 kb on - strand, within BWI76_RS22220 at 4633.729 kb on + strand, within BWI76_RS22225 at 4633.734 kb on + strand, within BWI76_RS22225 at 4633.793 kb on + strand, within BWI76_RS22225 at 4633.894 kb on + strand, within BWI76_RS22225 at 4633.921 kb on - strand, within BWI76_RS22225 at 4633.922 kb on + strand, within BWI76_RS22225 at 4633.977 kb on - strand, within BWI76_RS22225 at 4634.085 kb on + strand at 4634.184 kb on + strand, within BWI76_RS22230 at 4634.454 kb on - strand, within BWI76_RS22230 at 4634.508 kb on + strand, within BWI76_RS22230 at 4634.581 kb on + strand, within BWI76_RS22230 at 4634.664 kb on - strand, within BWI76_RS22230 at 4634.679 kb on - strand, within BWI76_RS22230 at 4635.182 kb on - strand, within BWI76_RS22235 at 4635.467 kb on - strand at 4635.495 kb on - strand, within BWI76_RS22240 at 4635.583 kb on - strand, within BWI76_RS22240 at 4635.705 kb on + strand, within BWI76_RS22240
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 4,633,055 + BWI76_RS22220 0.38 -0.4 4,633,335 - BWI76_RS22220 0.67 -1.3 4,633,346 - BWI76_RS22220 0.68 -0.0 4,633,415 + BWI76_RS22220 0.75 -0.2 4,633,551 + BWI76_RS22220 0.89 +0.5 4,633,559 - BWI76_RS22220 0.89 -0.8 4,633,559 - BWI76_RS22220 0.89 +0.1 4,633,729 + BWI76_RS22225 0.15 +0.5 4,633,734 + BWI76_RS22225 0.16 +0.3 4,633,793 + BWI76_RS22225 0.33 -0.6 4,633,894 + BWI76_RS22225 0.60 -0.2 4,633,921 - BWI76_RS22225 0.68 -0.8 4,633,922 + BWI76_RS22225 0.68 -0.9 4,633,977 - BWI76_RS22225 0.83 -0.3 4,634,085 + +0.4 4,634,184 + BWI76_RS22230 0.17 -0.2 4,634,454 - BWI76_RS22230 0.51 -0.6 4,634,508 + BWI76_RS22230 0.58 -0.7 4,634,581 + BWI76_RS22230 0.67 -0.2 4,634,664 - BWI76_RS22230 0.78 -0.8 4,634,679 - BWI76_RS22230 0.80 +0.2 4,635,182 - BWI76_RS22235 0.59 +1.5 4,635,467 - +0.9 4,635,495 - BWI76_RS22240 0.13 -0.0 4,635,583 - BWI76_RS22240 0.24 -0.9 4,635,705 + BWI76_RS22240 0.40 -0.9
Or see this region's nucleotide sequence