Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21490 and BWI76_RS21495 are separated by 11 nucleotides BWI76_RS21495 and BWI76_RS21500 are separated by 5 nucleotides BWI76_RS21500 and BWI76_RS21505 are separated by 54 nucleotides BWI76_RS21505 and BWI76_RS21510 are separated by 120 nucleotides BWI76_RS21510 and BWI76_RS21515 are separated by 39 nucleotides
BWI76_RS21490: BWI76_RS21490 - GGDEF domain-containing protein, at 4,501,737 to 4,502,960
_RS21490
BWI76_RS21495: BWI76_RS21495 - outer membrane lipoprotein, at 4,502,972 to 4,503,454
_RS21495
BWI76_RS21500: BWI76_RS21500 - membrane protein, at 4,503,460 to 4,504,830
_RS21500
BWI76_RS21505: BWI76_RS21505 - hypothetical protein, at 4,504,885 to 4,505,322
_RS21505
BWI76_RS21510: BWI76_RS21510 - 50S ribosomal protein L19, at 4,505,443 to 4,505,790
_RS21510
BWI76_RS21515: BWI76_RS21515 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 4,505,830 to 4,506,597
_RS21515
Position (kb) 4503
4504
4505 Strain fitness (log2 ratio) -2
-1
0
1 at 4502.466 kb on + strand, within BWI76_RS21490 at 4502.490 kb on + strand, within BWI76_RS21490 at 4502.504 kb on + strand, within BWI76_RS21490 at 4502.512 kb on - strand, within BWI76_RS21490 at 4502.543 kb on + strand, within BWI76_RS21490 at 4502.567 kb on - strand, within BWI76_RS21490 at 4502.569 kb on + strand, within BWI76_RS21490 at 4502.599 kb on + strand, within BWI76_RS21490 at 4502.600 kb on + strand, within BWI76_RS21490 at 4502.608 kb on - strand, within BWI76_RS21490 at 4502.701 kb on - strand, within BWI76_RS21490 at 4502.758 kb on - strand, within BWI76_RS21490 at 4502.763 kb on + strand, within BWI76_RS21490 at 4502.856 kb on - strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.867 kb on - strand at 4502.867 kb on - strand at 4502.867 kb on - strand at 4502.871 kb on + strand at 4502.879 kb on - strand at 4502.879 kb on - strand at 4502.884 kb on + strand at 4503.004 kb on - strand at 4503.089 kb on + strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.097 kb on - strand at 4503.195 kb on + strand, within BWI76_RS21495 at 4503.203 kb on - strand, within BWI76_RS21495 at 4503.208 kb on - strand at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.226 kb on + strand, within BWI76_RS21495 at 4503.240 kb on - strand, within BWI76_RS21495 at 4503.613 kb on - strand, within BWI76_RS21500 at 4503.647 kb on + strand, within BWI76_RS21500 at 4503.891 kb on + strand, within BWI76_RS21500 at 4504.376 kb on + strand, within BWI76_RS21500 at 4504.688 kb on - strand, within BWI76_RS21500 at 4504.802 kb on - strand at 4504.808 kb on - strand at 4504.940 kb on - strand, within BWI76_RS21505
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 4,502,466 + BWI76_RS21490 0.60 +0.9 4,502,490 + BWI76_RS21490 0.62 +0.6 4,502,504 + BWI76_RS21490 0.63 -0.0 4,502,512 - BWI76_RS21490 0.63 -0.1 4,502,543 + BWI76_RS21490 0.66 -0.8 4,502,567 - BWI76_RS21490 0.68 +0.8 4,502,569 + BWI76_RS21490 0.68 -0.8 4,502,599 + BWI76_RS21490 0.70 +0.1 4,502,600 + BWI76_RS21490 0.71 -0.3 4,502,608 - BWI76_RS21490 0.71 +0.9 4,502,701 - BWI76_RS21490 0.79 +0.5 4,502,758 - BWI76_RS21490 0.83 +0.3 4,502,763 + BWI76_RS21490 0.84 +0.8 4,502,856 - +0.1 4,502,859 + -0.6 4,502,859 + +0.5 4,502,859 + +0.4 4,502,859 + +0.6 4,502,867 - +0.4 4,502,867 - +1.7 4,502,867 - +0.3 4,502,871 + +0.7 4,502,879 - +1.2 4,502,879 - +0.5 4,502,884 + +0.9 4,503,004 - +0.2 4,503,089 + BWI76_RS21495 0.24 -2.3 4,503,092 - BWI76_RS21495 0.25 +1.4 4,503,092 - BWI76_RS21495 0.25 -0.8 4,503,092 - BWI76_RS21495 0.25 -0.6 4,503,092 - BWI76_RS21495 0.25 -1.0 4,503,097 - -0.6 4,503,195 + BWI76_RS21495 0.46 -0.6 4,503,203 - BWI76_RS21495 0.48 +1.4 4,503,208 - -0.6 4,503,224 - BWI76_RS21495 0.52 -0.4 4,503,224 - BWI76_RS21495 0.52 -1.7 4,503,224 - BWI76_RS21495 0.52 -0.3 4,503,226 + BWI76_RS21495 0.53 -0.2 4,503,240 - BWI76_RS21495 0.55 +0.2 4,503,613 - BWI76_RS21500 0.11 -1.7 4,503,647 + BWI76_RS21500 0.14 +1.1 4,503,891 + BWI76_RS21500 0.31 -1.5 4,504,376 + BWI76_RS21500 0.67 -0.6 4,504,688 - BWI76_RS21500 0.90 -0.6 4,504,802 - +0.4 4,504,808 - +0.8 4,504,940 - BWI76_RS21505 0.13 +1.0
Or see this region's nucleotide sequence