Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21220

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21215 and BWI76_RS21220 are separated by 73 nucleotidesBWI76_RS21220 and BWI76_RS21225 are separated by 55 nucleotides BWI76_RS21215: BWI76_RS21215 - NAGC-like transcriptional regulator, at 4,443,874 to 4,445,070 _RS21215 BWI76_RS21220: BWI76_RS21220 - membrane protein, at 4,445,144 to 4,445,566 _RS21220 BWI76_RS21225: BWI76_RS21225 - serine hydroxymethyltransferase, at 4,445,622 to 4,446,875 _RS21225 Position (kb) 4445 4446Strain fitness (log2 ratio) -2 -1 0 1 2at 4444.154 kb on - strand, within BWI76_RS21215at 4444.247 kb on + strand, within BWI76_RS21215at 4444.255 kb on - strand, within BWI76_RS21215at 4444.411 kb on - strand, within BWI76_RS21215at 4444.755 kb on + strand, within BWI76_RS21215at 4444.887 kb on - strand, within BWI76_RS21215at 4444.934 kb on + strand, within BWI76_RS21215at 4445.027 kb on - strandat 4445.165 kb on + strandat 4445.168 kb on - strandat 4445.270 kb on + strand, within BWI76_RS21220at 4445.283 kb on - strand, within BWI76_RS21220at 4445.423 kb on + strand, within BWI76_RS21220at 4445.519 kb on - strand, within BWI76_RS21220at 4445.573 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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4,444,154 - BWI76_RS21215 0.23 +0.5
4,444,247 + BWI76_RS21215 0.31 -0.3
4,444,255 - BWI76_RS21215 0.32 -0.6
4,444,411 - BWI76_RS21215 0.45 +1.4
4,444,755 + BWI76_RS21215 0.74 -0.1
4,444,887 - BWI76_RS21215 0.85 +0.0
4,444,934 + BWI76_RS21215 0.89 -0.2
4,445,027 - +1.4
4,445,165 + -2.3
4,445,168 - +2.1
4,445,270 + BWI76_RS21220 0.30 -1.1
4,445,283 - BWI76_RS21220 0.33 -2.0
4,445,423 + BWI76_RS21220 0.66 +0.1
4,445,519 - BWI76_RS21220 0.89 -0.6
4,445,573 - +0.6

Or see this region's nucleotide sequence