Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20830 and BWI76_RS20835 are separated by 289 nucleotides BWI76_RS20835 and BWI76_RS20840 overlap by 32 nucleotides
BWI76_RS20830: BWI76_RS20830 - bifunctional malic enzyme oxidoreductase/phosphotransacetylase, at 4,351,514 to 4,353,793
_RS20830
BWI76_RS20835: BWI76_RS20835 - transketolase, at 4,354,083 to 4,356,077
_RS20835
BWI76_RS20840: BWI76_RS20840 - hypothetical protein, at 4,356,046 to 4,357,113
_RS20840
Position (kb)
4354
4355
4356
4357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4353.310 kb on - strand, within BWI76_RS20830 at 4353.401 kb on + strand, within BWI76_RS20830 at 4353.638 kb on - strand at 4353.847 kb on - strand at 4353.856 kb on + strand at 4353.865 kb on - strand at 4353.905 kb on - strand at 4354.263 kb on - strand at 4354.287 kb on - strand, within BWI76_RS20835 at 4354.289 kb on + strand, within BWI76_RS20835 at 4354.289 kb on + strand, within BWI76_RS20835 at 4354.465 kb on - strand, within BWI76_RS20835 at 4354.532 kb on - strand, within BWI76_RS20835 at 4354.602 kb on - strand, within BWI76_RS20835 at 4354.602 kb on - strand, within BWI76_RS20835 at 4354.656 kb on + strand, within BWI76_RS20835 at 4354.793 kb on - strand, within BWI76_RS20835 at 4355.025 kb on - strand, within BWI76_RS20835 at 4355.134 kb on - strand, within BWI76_RS20835 at 4355.239 kb on - strand, within BWI76_RS20835 at 4355.378 kb on - strand, within BWI76_RS20835 at 4355.450 kb on + strand, within BWI76_RS20835 at 4355.603 kb on + strand, within BWI76_RS20835 at 4355.803 kb on - strand, within BWI76_RS20835 at 4355.803 kb on - strand, within BWI76_RS20835 at 4355.858 kb on - strand, within BWI76_RS20835 at 4356.071 kb on - strand at 4356.080 kb on - strand at 4356.279 kb on + strand, within BWI76_RS20840 at 4356.306 kb on + strand, within BWI76_RS20840 at 4356.337 kb on - strand, within BWI76_RS20840 at 4356.674 kb on + strand, within BWI76_RS20840 at 4356.719 kb on + strand, within BWI76_RS20840 at 4356.742 kb on - strand, within BWI76_RS20840 at 4356.930 kb on + strand, within BWI76_RS20840
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 4,353,310 - BWI76_RS20830 0.79 +0.9 4,353,401 + BWI76_RS20830 0.83 -0.8 4,353,638 - +0.1 4,353,847 - -0.7 4,353,856 + +1.4 4,353,865 - +0.6 4,353,905 - -0.6 4,354,263 - -1.0 4,354,287 - BWI76_RS20835 0.10 +0.0 4,354,289 + BWI76_RS20835 0.10 +0.6 4,354,289 + BWI76_RS20835 0.10 -1.9 4,354,465 - BWI76_RS20835 0.19 -2.1 4,354,532 - BWI76_RS20835 0.23 -1.1 4,354,602 - BWI76_RS20835 0.26 -0.1 4,354,602 - BWI76_RS20835 0.26 -1.1 4,354,656 + BWI76_RS20835 0.29 -0.3 4,354,793 - BWI76_RS20835 0.36 -0.5 4,355,025 - BWI76_RS20835 0.47 +0.2 4,355,134 - BWI76_RS20835 0.53 +1.4 4,355,239 - BWI76_RS20835 0.58 -0.2 4,355,378 - BWI76_RS20835 0.65 -0.0 4,355,450 + BWI76_RS20835 0.69 +1.9 4,355,603 + BWI76_RS20835 0.76 -0.2 4,355,803 - BWI76_RS20835 0.86 -0.6 4,355,803 - BWI76_RS20835 0.86 +0.3 4,355,858 - BWI76_RS20835 0.89 -0.2 4,356,071 - -1.2 4,356,080 - -3.1 4,356,279 + BWI76_RS20840 0.22 -0.1 4,356,306 + BWI76_RS20840 0.24 +0.5 4,356,337 - BWI76_RS20840 0.27 +1.3 4,356,674 + BWI76_RS20840 0.59 +1.1 4,356,719 + BWI76_RS20840 0.63 +0.3 4,356,742 - BWI76_RS20840 0.65 -0.8 4,356,930 + BWI76_RS20840 0.83 +0.5
Or see this region's nucleotide sequence