Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16850 and BWI76_RS16855 overlap by 4 nucleotides BWI76_RS16855 and BWI76_RS16860 are separated by 11 nucleotides BWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotides
BWI76_RS16850: BWI76_RS16850 - ABC transporter periplasmic substrate-binding protein, at 3,494,332 to 3,495,369
_RS16850
BWI76_RS16855: BWI76_RS16855 - putative ABC transporter periplasmic solute-binding protein, at 3,495,366 to 3,496,340
_RS16855
BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440
_RS16860
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-1
0
1 at 3495.367 kb on - strand at 3495.387 kb on + strand at 3495.787 kb on + strand, within BWI76_RS16855 at 3496.282 kb on - strand at 3496.429 kb on - strand at 3496.666 kb on + strand, within BWI76_RS16860 at 3496.720 kb on - strand, within BWI76_RS16860 at 3497.655 kb on + strand, within BWI76_RS16865 at 3497.663 kb on + strand, within BWI76_RS16865 at 3497.663 kb on - strand, within BWI76_RS16865 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 3,495,367 - +0.7 3,495,387 + -1.6 3,495,787 + BWI76_RS16855 0.43 -0.1 3,496,282 - -1.0 3,496,429 - -1.2 3,496,666 + BWI76_RS16860 0.29 -1.1 3,496,720 - BWI76_RS16860 0.34 -1.3 3,497,655 + BWI76_RS16865 0.15 +0.3 3,497,663 + BWI76_RS16865 0.16 -0.5 3,497,663 - BWI76_RS16865 0.16 -0.9 3,497,738 + BWI76_RS16865 0.22 -0.1 3,498,029 + BWI76_RS16865 0.49 -0.1 3,498,154 + BWI76_RS16865 0.61 -0.2 3,498,173 + +0.5 3,498,181 - BWI76_RS16865 0.63 -0.1
Or see this region's nucleotide sequence