Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16175

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16170 and BWI76_RS16175 are separated by 111 nucleotidesBWI76_RS16175 and BWI76_RS16180 are separated by 453 nucleotides BWI76_RS16170: BWI76_RS16170 - amidohydrolase, at 3,354,337 to 3,355,782 _RS16170 BWI76_RS16175: BWI76_RS16175 - p-aminobenzoyl-glutamate transporter, at 3,355,894 to 3,357,420 _RS16175 BWI76_RS16180: BWI76_RS16180 - methylated-DNA--protein-cysteine methyltransferase, at 3,357,874 to 3,358,389 _RS16180 Position (kb) 3355 3356 3357 3358Strain fitness (log2 ratio) -1 0 1at 3355.081 kb on + strand, within BWI76_RS16170at 3355.197 kb on - strand, within BWI76_RS16170at 3355.441 kb on - strand, within BWI76_RS16170at 3355.724 kb on - strandat 3355.724 kb on - strandat 3355.766 kb on - strandat 3355.905 kb on + strandat 3355.912 kb on + strandat 3355.943 kb on - strandat 3356.652 kb on + strand, within BWI76_RS16175at 3356.652 kb on + strand, within BWI76_RS16175at 3356.677 kb on - strand, within BWI76_RS16175at 3356.680 kb on + strand, within BWI76_RS16175at 3357.431 kb on + strandat 3357.603 kb on - strandat 3357.644 kb on + strandat 3357.686 kb on + strandat 3357.899 kb on - strandat 3358.274 kb on + strand, within BWI76_RS16180at 3358.344 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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3,355,081 + BWI76_RS16170 0.51 +0.3
3,355,197 - BWI76_RS16170 0.59 +0.0
3,355,441 - BWI76_RS16170 0.76 -0.5
3,355,724 - +0.1
3,355,724 - -0.8
3,355,766 - +1.7
3,355,905 + -1.4
3,355,912 + -0.0
3,355,943 - +0.7
3,356,652 + BWI76_RS16175 0.50 -1.5
3,356,652 + BWI76_RS16175 0.50 +0.1
3,356,677 - BWI76_RS16175 0.51 -1.0
3,356,680 + BWI76_RS16175 0.51 -1.1
3,357,431 + +0.7
3,357,603 - -0.0
3,357,644 + +0.6
3,357,686 + +1.0
3,357,899 - -0.8
3,358,274 + BWI76_RS16180 0.78 -0.0
3,358,344 + +0.6

Or see this region's nucleotide sequence