Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15655 and BWI76_RS15660 overlap by 14 nucleotides BWI76_RS15660 and BWI76_RS15665 are separated by 31 nucleotides BWI76_RS15665 and BWI76_RS15670 are separated by 274 nucleotides BWI76_RS15670 and BWI76_RS15675 are separated by 69 nucleotides BWI76_RS15675 and BWI76_RS15680 are separated by 111 nucleotides
BWI76_RS15655: BWI76_RS15655 - hypothetical protein, at 3,271,852 to 3,273,330
_RS15655
BWI76_RS15660: BWI76_RS15660 - hypothetical protein, at 3,273,317 to 3,273,796
_RS15660
BWI76_RS15665: BWI76_RS15665 - hypothetical protein, at 3,273,828 to 3,274,475
_RS15665
BWI76_RS15670: BWI76_RS15670 - hypothetical protein, at 3,274,750 to 3,274,950
_RS15670
BWI76_RS15675: BWI76_RS15675 - hypothetical protein, at 3,275,020 to 3,275,265
_RS15675
BWI76_RS15680: BWI76_RS15680 - hypothetical protein, at 3,275,377 to 3,275,562
_RS15680
Position (kb)
3273
3274
3275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3272.936 kb on + strand, within BWI76_RS15655 at 3272.993 kb on + strand, within BWI76_RS15655 at 3273.020 kb on - strand, within BWI76_RS15655 at 3273.088 kb on + strand, within BWI76_RS15655 at 3273.123 kb on + strand, within BWI76_RS15655 at 3273.196 kb on + strand at 3273.209 kb on - strand at 3273.415 kb on + strand, within BWI76_RS15660 at 3273.809 kb on + strand at 3273.817 kb on - strand at 3273.817 kb on - strand at 3273.867 kb on - strand at 3273.918 kb on - strand, within BWI76_RS15665 at 3274.043 kb on - strand, within BWI76_RS15665 at 3274.069 kb on + strand, within BWI76_RS15665 at 3274.327 kb on + strand, within BWI76_RS15665 at 3274.458 kb on + strand at 3274.487 kb on - strand at 3274.489 kb on - strand at 3274.494 kb on - strand at 3274.621 kb on + strand at 3274.867 kb on - strand, within BWI76_RS15670 at 3274.872 kb on + strand, within BWI76_RS15670 at 3274.872 kb on + strand, within BWI76_RS15670 at 3274.894 kb on - strand, within BWI76_RS15670 at 3275.005 kb on + strand at 3275.008 kb on - strand at 3275.165 kb on + strand, within BWI76_RS15675 at 3275.186 kb on + strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.194 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.221 kb on - strand, within BWI76_RS15675 at 3275.278 kb on + strand at 3275.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 3,272,936 + BWI76_RS15655 0.73 +1.9 3,272,993 + BWI76_RS15655 0.77 -4.0 3,273,020 - BWI76_RS15655 0.79 +0.3 3,273,088 + BWI76_RS15655 0.84 +0.2 3,273,123 + BWI76_RS15655 0.86 -1.3 3,273,196 + -0.5 3,273,209 - +1.1 3,273,415 + BWI76_RS15660 0.20 +0.5 3,273,809 + +1.9 3,273,817 - -0.8 3,273,817 - +1.5 3,273,867 - +0.5 3,273,918 - BWI76_RS15665 0.14 -1.0 3,274,043 - BWI76_RS15665 0.33 -1.1 3,274,069 + BWI76_RS15665 0.37 -0.5 3,274,327 + BWI76_RS15665 0.77 -0.5 3,274,458 + +0.4 3,274,487 - +0.1 3,274,489 - -1.5 3,274,494 - -1.8 3,274,621 + -0.5 3,274,867 - BWI76_RS15670 0.58 -0.2 3,274,872 + BWI76_RS15670 0.61 +1.5 3,274,872 + BWI76_RS15670 0.61 -0.3 3,274,894 - BWI76_RS15670 0.72 -0.4 3,275,005 + +0.7 3,275,008 - -0.8 3,275,165 + BWI76_RS15675 0.59 +0.6 3,275,186 + BWI76_RS15675 0.67 -0.5 3,275,194 - BWI76_RS15675 0.71 -0.3 3,275,194 - BWI76_RS15675 0.71 +0.3 3,275,194 - BWI76_RS15675 0.71 -0.5 3,275,221 - BWI76_RS15675 0.82 +1.0 3,275,221 - BWI76_RS15675 0.82 +0.3 3,275,278 + +1.9 3,275,322 - +1.5
Or see this region's nucleotide sequence