Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15590 and BWI76_RS15595 are separated by 59 nucleotides BWI76_RS15595 and BWI76_RS15600 are separated by 58 nucleotides BWI76_RS15600 and BWI76_RS15605 overlap by 4 nucleotides BWI76_RS15605 and BWI76_RS15610 are separated by 2 nucleotides BWI76_RS15610 and BWI76_RS15615 are separated by 2 nucleotides BWI76_RS15615 and BWI76_RS15620 overlap by 1 nucleotides
BWI76_RS15590: BWI76_RS15590 - hypothetical protein, at 3,263,250 to 3,263,957
_RS15590
BWI76_RS15595: BWI76_RS15595 - hypothetical protein, at 3,264,017 to 3,264,781
_RS15595
BWI76_RS15600: BWI76_RS15600 - hypothetical protein, at 3,264,840 to 3,265,223
_RS15600
BWI76_RS15605: BWI76_RS15605 - hypothetical protein, at 3,265,220 to 3,265,588
_RS15605
BWI76_RS15610: BWI76_RS15610 - hypothetical protein, at 3,265,591 to 3,265,953
_RS15610
BWI76_RS15615: BWI76_RS15615 - hypothetical protein, at 3,265,956 to 3,266,126
_RS15615
BWI76_RS15620: BWI76_RS15620 - hypothetical protein, at 3,266,126 to 3,266,527
_RS15620
Position (kb) 3264
3265
3266 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3264.104 kb on - strand, within BWI76_RS15595 at 3264.185 kb on + strand, within BWI76_RS15595 at 3264.185 kb on + strand, within BWI76_RS15595 at 3264.312 kb on + strand, within BWI76_RS15595 at 3264.444 kb on - strand, within BWI76_RS15595 at 3264.675 kb on - strand, within BWI76_RS15595 at 3264.713 kb on + strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.721 kb on - strand at 3264.781 kb on - strand at 3264.869 kb on + strand at 3264.898 kb on + strand, within BWI76_RS15600 at 3265.209 kb on + strand at 3265.292 kb on - strand, within BWI76_RS15605 at 3265.517 kb on - strand, within BWI76_RS15605 at 3266.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 3,264,104 - BWI76_RS15595 0.11 +1.1 3,264,185 + BWI76_RS15595 0.22 -0.5 3,264,185 + BWI76_RS15595 0.22 +0.1 3,264,312 + BWI76_RS15595 0.39 +0.4 3,264,444 - BWI76_RS15595 0.56 -0.1 3,264,675 - BWI76_RS15595 0.86 +0.8 3,264,713 + +0.3 3,264,721 - +1.1 3,264,721 - -0.5 3,264,721 - -1.1 3,264,721 - +0.1 3,264,781 - -0.3 3,264,869 + -0.1 3,264,898 + BWI76_RS15600 0.15 -1.4 3,265,209 + -0.1 3,265,292 - BWI76_RS15605 0.20 -0.9 3,265,517 - BWI76_RS15605 0.80 -3.2 3,266,131 - +3.5
Or see this region's nucleotide sequence