Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14730 and BWI76_RS14735 are separated by 112 nucleotides BWI76_RS14735 and BWI76_RS14740 are separated by 73 nucleotides BWI76_RS14740 and BWI76_RS14745 are separated by 96 nucleotides
BWI76_RS14730: BWI76_RS14730 - 4-oxalocrotonate tautomerase, at 3,091,927 to 3,092,163
_RS14730
BWI76_RS14735: BWI76_RS14735 - type 11 methyltransferase, at 3,092,276 to 3,092,944
_RS14735
BWI76_RS14740: BWI76_RS14740 - LysR family transcriptional regulator, at 3,093,018 to 3,093,905
_RS14740
BWI76_RS14745: BWI76_RS14745 - NAD-dependent epimerase/dehydratase, at 3,094,002 to 3,095,057
_RS14745
Position (kb)
3093
3094 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3092.064 kb on - strand, within BWI76_RS14730 at 3092.066 kb on + strand, within BWI76_RS14730 at 3092.068 kb on - strand, within BWI76_RS14730 at 3092.081 kb on + strand, within BWI76_RS14730 at 3092.112 kb on + strand, within BWI76_RS14730 at 3092.120 kb on - strand, within BWI76_RS14730 at 3092.217 kb on - strand at 3092.288 kb on + strand at 3092.296 kb on - strand at 3092.318 kb on - strand at 3092.610 kb on + strand, within BWI76_RS14735 at 3092.753 kb on - strand, within BWI76_RS14735 at 3092.782 kb on - strand, within BWI76_RS14735 at 3093.271 kb on + strand, within BWI76_RS14740 at 3093.381 kb on + strand at 3093.389 kb on - strand, within BWI76_RS14740 at 3093.542 kb on - strand, within BWI76_RS14740 at 3093.756 kb on - strand, within BWI76_RS14740 at 3093.774 kb on - strand, within BWI76_RS14740 at 3094.027 kb on - strand at 3094.181 kb on - strand, within BWI76_RS14745 at 3094.231 kb on + strand, within BWI76_RS14745 at 3094.233 kb on + strand, within BWI76_RS14745 at 3094.333 kb on + strand, within BWI76_RS14745 at 3094.341 kb on - strand, within BWI76_RS14745 at 3094.341 kb on - strand, within BWI76_RS14745 at 3094.440 kb on + strand, within BWI76_RS14745 at 3094.484 kb on + strand, within BWI76_RS14745 at 3094.612 kb on + strand, within BWI76_RS14745 at 3094.625 kb on - strand, within BWI76_RS14745 at 3094.636 kb on - strand, within BWI76_RS14745 at 3094.747 kb on + strand, within BWI76_RS14745 at 3094.785 kb on - strand, within BWI76_RS14745 at 3094.795 kb on - strand, within BWI76_RS14745 at 3094.813 kb on + strand, within BWI76_RS14745
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 3,092,064 - BWI76_RS14730 0.58 -0.3 3,092,066 + BWI76_RS14730 0.59 +0.1 3,092,068 - BWI76_RS14730 0.59 -2.9 3,092,081 + BWI76_RS14730 0.65 +0.0 3,092,112 + BWI76_RS14730 0.78 -0.7 3,092,120 - BWI76_RS14730 0.81 +1.0 3,092,217 - +2.3 3,092,288 + +0.4 3,092,296 - -0.3 3,092,318 - -0.2 3,092,610 + BWI76_RS14735 0.50 +0.3 3,092,753 - BWI76_RS14735 0.71 +0.6 3,092,782 - BWI76_RS14735 0.76 -0.4 3,093,271 + BWI76_RS14740 0.28 -0.1 3,093,381 + +0.5 3,093,389 - BWI76_RS14740 0.42 -1.1 3,093,542 - BWI76_RS14740 0.59 -2.8 3,093,756 - BWI76_RS14740 0.83 -1.4 3,093,774 - BWI76_RS14740 0.85 -1.5 3,094,027 - +1.5 3,094,181 - BWI76_RS14745 0.17 +0.8 3,094,231 + BWI76_RS14745 0.22 +0.3 3,094,233 + BWI76_RS14745 0.22 +2.5 3,094,333 + BWI76_RS14745 0.31 -1.4 3,094,341 - BWI76_RS14745 0.32 +2.1 3,094,341 - BWI76_RS14745 0.32 -1.1 3,094,440 + BWI76_RS14745 0.41 +0.3 3,094,484 + BWI76_RS14745 0.46 -0.1 3,094,612 + BWI76_RS14745 0.58 +0.1 3,094,625 - BWI76_RS14745 0.59 -0.3 3,094,636 - BWI76_RS14745 0.60 +1.0 3,094,747 + BWI76_RS14745 0.71 -0.4 3,094,785 - BWI76_RS14745 0.74 -0.9 3,094,795 - BWI76_RS14745 0.75 +0.1 3,094,813 + BWI76_RS14745 0.77 -1.1
Or see this region's nucleotide sequence