Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11775 and BWI76_RS11780 are separated by 148 nucleotides BWI76_RS11780 and BWI76_RS11785 are separated by 130 nucleotides BWI76_RS11785 and BWI76_RS11790 are separated by 32 nucleotides
BWI76_RS11775: BWI76_RS11775 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,477,535 to 2,478,296
_RS11775
BWI76_RS11780: BWI76_RS11780 - 2-deoxyglucose-6-phosphatase, at 2,478,445 to 2,479,113
_RS11780
BWI76_RS11785: BWI76_RS11785 - hypothetical protein, at 2,479,244 to 2,479,780
_RS11785
BWI76_RS11790: BWI76_RS11790 - acyl-CoA thioesterase, at 2,479,813 to 2,480,211
_RS11790
Position (kb)
2478
2479
2480 Strain fitness (log2 ratio)
-1
0
1 at 2477.451 kb on + strand at 2477.575 kb on - strand at 2477.628 kb on - strand, within BWI76_RS11775 at 2477.829 kb on - strand, within BWI76_RS11775 at 2478.275 kb on + strand at 2478.283 kb on - strand at 2478.340 kb on + strand at 2478.613 kb on + strand, within BWI76_RS11780 at 2478.926 kb on + strand, within BWI76_RS11780 at 2479.249 kb on - strand at 2479.249 kb on - strand at 2479.401 kb on + strand, within BWI76_RS11785
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 2,477,451 + +0.5 2,477,575 - +1.0 2,477,628 - BWI76_RS11775 0.12 -0.4 2,477,829 - BWI76_RS11775 0.39 -0.4 2,478,275 + -0.1 2,478,283 - -1.1 2,478,340 + -1.5 2,478,613 + BWI76_RS11780 0.25 -1.1 2,478,926 + BWI76_RS11780 0.72 -0.9 2,479,249 - -0.5 2,479,249 - +0.4 2,479,401 + BWI76_RS11785 0.29 +1.0
Or see this region's nucleotide sequence