Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10685 and BWI76_RS10690 overlap by 4 nucleotides BWI76_RS10690 and BWI76_RS10695 are separated by 2 nucleotides
BWI76_RS10685: BWI76_RS10685 - LysR family transcriptional regulator, at 2,257,894 to 2,258,850
_RS10685
BWI76_RS10690: BWI76_RS10690 - gamma-glutamylputrescine oxidoreductase, at 2,258,847 to 2,260,127
_RS10690
BWI76_RS10695: BWI76_RS10695 - aldehyde dehydrogenase PuuC, at 2,260,130 to 2,261,617
_RS10695
Position (kb)
2258
2259
2260
2261 Strain fitness (log2 ratio)
-1
0
1 at 2257.928 kb on + strand at 2257.928 kb on + strand at 2258.359 kb on - strand, within BWI76_RS10685 at 2258.458 kb on + strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.537 kb on + strand, within BWI76_RS10685 at 2259.014 kb on + strand, within BWI76_RS10690 at 2259.037 kb on + strand, within BWI76_RS10690 at 2259.456 kb on - strand, within BWI76_RS10690 at 2259.729 kb on - strand, within BWI76_RS10690 at 2259.731 kb on - strand, within BWI76_RS10690 at 2259.828 kb on + strand, within BWI76_RS10690 at 2259.909 kb on - strand, within BWI76_RS10690 at 2260.534 kb on - strand, within BWI76_RS10695 at 2260.554 kb on - strand, within BWI76_RS10695 at 2260.643 kb on - strand, within BWI76_RS10695 at 2260.715 kb on + strand, within BWI76_RS10695 at 2260.715 kb on + strand, within BWI76_RS10695 at 2260.771 kb on + strand, within BWI76_RS10695 at 2260.845 kb on - strand, within BWI76_RS10695 at 2261.044 kb on + strand, within BWI76_RS10695
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 2,257,928 + -0.5 2,257,928 + -1.3 2,258,359 - BWI76_RS10685 0.49 -0.7 2,258,458 + BWI76_RS10685 0.59 -0.3 2,258,466 - BWI76_RS10685 0.60 -1.3 2,258,466 - BWI76_RS10685 0.60 -0.6 2,258,466 - BWI76_RS10685 0.60 -0.4 2,258,537 + BWI76_RS10685 0.67 -0.4 2,259,014 + BWI76_RS10690 0.13 +1.0 2,259,037 + BWI76_RS10690 0.15 +0.4 2,259,456 - BWI76_RS10690 0.48 +0.0 2,259,729 - BWI76_RS10690 0.69 -0.2 2,259,731 - BWI76_RS10690 0.69 +0.2 2,259,828 + BWI76_RS10690 0.77 +0.4 2,259,909 - BWI76_RS10690 0.83 +1.2 2,260,534 - BWI76_RS10695 0.27 +0.2 2,260,554 - BWI76_RS10695 0.28 +0.1 2,260,643 - BWI76_RS10695 0.34 -0.4 2,260,715 + BWI76_RS10695 0.39 -0.1 2,260,715 + BWI76_RS10695 0.39 -0.5 2,260,771 + BWI76_RS10695 0.43 +0.9 2,260,845 - BWI76_RS10695 0.48 -0.5 2,261,044 + BWI76_RS10695 0.61 +1.0
Or see this region's nucleotide sequence