Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04105

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04100 and BWI76_RS04105 are separated by 217 nucleotidesBWI76_RS04105 and BWI76_RS04110 are separated by 123 nucleotidesBWI76_RS04110 and BWI76_RS04115 are separated by 120 nucleotides BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646 _RS04100 BWI76_RS04105: BWI76_RS04105 - molecular chaperone OsmY, at 844,864 to 845,475 _RS04105 BWI76_RS04110: BWI76_RS04110 - DUF1328 domain-containing protein, at 845,599 to 845,760 _RS04110 BWI76_RS04115: BWI76_RS04115 - patatin family protein, at 845,881 to 846,954 _RS04115 Position (kb) 844 845 846Strain fitness (log2 ratio) -2 -1 0 1 2at 843.868 kb on + strand, within BWI76_RS04100at 844.010 kb on - strand, within BWI76_RS04100at 844.121 kb on + strand, within BWI76_RS04100at 844.189 kb on + strand, within BWI76_RS04100at 844.196 kb on - strand, within BWI76_RS04100at 844.247 kb on + strand, within BWI76_RS04100at 844.482 kb on - strand, within BWI76_RS04100at 844.899 kb on - strandat 844.923 kb on - strandat 844.951 kb on + strand, within BWI76_RS04105at 844.951 kb on + strand, within BWI76_RS04105at 844.951 kb on + strand, within BWI76_RS04105at 844.959 kb on - strand, within BWI76_RS04105at 845.063 kb on - strand, within BWI76_RS04105at 845.063 kb on - strand, within BWI76_RS04105at 845.097 kb on + strand, within BWI76_RS04105at 845.133 kb on - strand, within BWI76_RS04105at 845.141 kb on + strand, within BWI76_RS04105at 845.259 kb on + strand, within BWI76_RS04105at 845.368 kb on - strand, within BWI76_RS04105at 845.385 kb on - strand, within BWI76_RS04105at 845.409 kb on + strand, within BWI76_RS04105at 845.436 kb on - strandat 845.527 kb on + strandat 845.527 kb on + strandat 845.527 kb on + strandat 845.527 kb on - strandat 845.535 kb on - strandat 845.535 kb on - strandat 845.540 kb on - strandat 845.582 kb on + strandat 845.589 kb on + strandat 845.590 kb on - strandat 845.647 kb on + strand, within BWI76_RS04110at 845.655 kb on - strand, within BWI76_RS04110at 845.657 kb on + strand, within BWI76_RS04110at 845.667 kb on + strand, within BWI76_RS04110at 845.687 kb on - strand, within BWI76_RS04110at 845.711 kb on + strand, within BWI76_RS04110at 845.763 kb on - strandat 845.907 kb on + strandat 845.912 kb on + strandat 845.951 kb on + strandat 845.959 kb on - strandat 845.959 kb on - strandat 845.964 kb on - strandat 845.971 kb on + strandat 845.980 kb on - strandat 846.420 kb on - strand, within BWI76_RS04115

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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843,868 + BWI76_RS04100 0.51 +0.8
844,010 - BWI76_RS04100 0.60 +1.6
844,121 + BWI76_RS04100 0.67 +0.7
844,189 + BWI76_RS04100 0.71 -1.2
844,196 - BWI76_RS04100 0.72 -1.8
844,247 + BWI76_RS04100 0.75 -0.3
844,482 - BWI76_RS04100 0.90 -0.9
844,899 - -1.4
844,923 - -1.3
844,951 + BWI76_RS04105 0.14 -1.7
844,951 + BWI76_RS04105 0.14 -0.4
844,951 + BWI76_RS04105 0.14 -1.2
844,959 - BWI76_RS04105 0.16 -0.2
845,063 - BWI76_RS04105 0.33 +0.3
845,063 - BWI76_RS04105 0.33 -1.5
845,097 + BWI76_RS04105 0.38 -0.5
845,133 - BWI76_RS04105 0.44 -0.9
845,141 + BWI76_RS04105 0.45 +0.0
845,259 + BWI76_RS04105 0.65 -0.5
845,368 - BWI76_RS04105 0.82 -0.6
845,385 - BWI76_RS04105 0.85 -0.4
845,409 + BWI76_RS04105 0.89 +0.4
845,436 - -0.1
845,527 + +0.3
845,527 + +1.2
845,527 + -0.2
845,527 - +0.6
845,535 - -0.4
845,535 - +1.6
845,540 - -1.2
845,582 + -0.6
845,589 + +2.0
845,590 - +0.3
845,647 + BWI76_RS04110 0.30 +1.2
845,655 - BWI76_RS04110 0.35 -0.6
845,657 + BWI76_RS04110 0.36 -0.5
845,667 + BWI76_RS04110 0.42 -1.3
845,687 - BWI76_RS04110 0.54 -0.9
845,711 + BWI76_RS04110 0.69 -1.0
845,763 - +0.0
845,907 + -0.2
845,912 + -0.2
845,951 + -2.4
845,959 - -0.4
845,959 - -0.9
845,964 - -0.1
845,971 + -0.7
845,980 - +2.3
846,420 - BWI76_RS04115 0.50 -1.0

Or see this region's nucleotide sequence