Experiment: KLEB30 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02040 and BWI76_RS02045 are separated by 60 nucleotides BWI76_RS02045 and BWI76_RS02050 are separated by 103 nucleotides BWI76_RS02050 and BWI76_RS02055 overlap by 8 nucleotides
BWI76_RS02040: BWI76_RS02040 - aryl-alcohol dehydrogenase, at 420,210 to 421,337
_RS02040
BWI76_RS02045: BWI76_RS02045 - LysR family transcriptional regulator, at 421,398 to 422,294
_RS02045
BWI76_RS02050: BWI76_RS02050 - CidA/LrgA family protein, at 422,398 to 422,808
_RS02050
BWI76_RS02055: BWI76_RS02055 - murein hydrolase effector protein LrgB, at 422,801 to 423,490
_RS02055
Position (kb) 421
422
423 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 420.593 kb on - strand, within BWI76_RS02040 at 420.600 kb on - strand, within BWI76_RS02040 at 420.600 kb on - strand, within BWI76_RS02040 at 420.688 kb on - strand, within BWI76_RS02040 at 420.722 kb on + strand, within BWI76_RS02040 at 420.773 kb on - strand, within BWI76_RS02040 at 420.857 kb on + strand, within BWI76_RS02040 at 420.899 kb on - strand, within BWI76_RS02040 at 420.915 kb on + strand, within BWI76_RS02040 at 421.033 kb on - strand, within BWI76_RS02040 at 421.033 kb on - strand, within BWI76_RS02040 at 421.118 kb on + strand, within BWI76_RS02040 at 421.118 kb on + strand, within BWI76_RS02040 at 421.149 kb on + strand, within BWI76_RS02040 at 421.157 kb on - strand, within BWI76_RS02040 at 421.157 kb on - strand, within BWI76_RS02040 at 421.177 kb on - strand, within BWI76_RS02040 at 421.212 kb on - strand, within BWI76_RS02040 at 421.233 kb on - strand at 421.252 kb on - strand at 421.270 kb on + strand at 421.284 kb on + strand at 421.284 kb on + strand at 421.343 kb on + strand at 421.359 kb on - strand at 421.387 kb on - strand at 421.395 kb on + strand at 421.395 kb on + strand at 421.403 kb on - strand at 421.438 kb on + strand at 421.477 kb on - strand at 421.478 kb on - strand at 421.526 kb on - strand, within BWI76_RS02045 at 421.540 kb on - strand, within BWI76_RS02045 at 421.626 kb on - strand, within BWI76_RS02045 at 421.950 kb on + strand, within BWI76_RS02045 at 422.000 kb on + strand, within BWI76_RS02045 at 422.148 kb on + strand, within BWI76_RS02045 at 422.161 kb on - strand, within BWI76_RS02045 at 422.308 kb on + strand at 422.316 kb on - strand at 422.316 kb on - strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.356 kb on + strand at 422.356 kb on + strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.368 kb on - strand at 422.415 kb on - strand at 422.418 kb on + strand at 422.429 kb on + strand at 422.429 kb on + strand at 422.437 kb on - strand at 422.461 kb on + strand, within BWI76_RS02050 at 422.496 kb on + strand, within BWI76_RS02050 at 422.782 kb on + strand at 422.792 kb on + strand at 422.792 kb on + strand at 422.792 kb on + strand at 422.816 kb on - strand at 422.978 kb on - strand, within BWI76_RS02055 at 423.081 kb on + strand, within BWI76_RS02055 at 423.182 kb on + strand, within BWI76_RS02055 at 423.271 kb on - strand, within BWI76_RS02055
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB30 MOI 1 remove 420,593 - BWI76_RS02040 0.34 +1.1 420,600 - BWI76_RS02040 0.35 -0.8 420,600 - BWI76_RS02040 0.35 +0.0 420,688 - BWI76_RS02040 0.42 +1.4 420,722 + BWI76_RS02040 0.45 -1.7 420,773 - BWI76_RS02040 0.50 -0.1 420,857 + BWI76_RS02040 0.57 +0.7 420,899 - BWI76_RS02040 0.61 -0.7 420,915 + BWI76_RS02040 0.62 +0.1 421,033 - BWI76_RS02040 0.73 -0.8 421,033 - BWI76_RS02040 0.73 -3.0 421,118 + BWI76_RS02040 0.80 +1.8 421,118 + BWI76_RS02040 0.80 +2.4 421,149 + BWI76_RS02040 0.83 -3.0 421,157 - BWI76_RS02040 0.84 -0.8 421,157 - BWI76_RS02040 0.84 -0.4 421,177 - BWI76_RS02040 0.86 +0.4 421,212 - BWI76_RS02040 0.89 -0.9 421,233 - +2.0 421,252 - -0.4 421,270 + +1.0 421,284 + -0.5 421,284 + +0.1 421,343 + -0.3 421,359 - -1.5 421,387 - -0.2 421,395 + +0.2 421,395 + +0.6 421,403 - +1.4 421,438 + -0.4 421,477 - -1.5 421,478 - +0.2 421,526 - BWI76_RS02045 0.14 -1.0 421,540 - BWI76_RS02045 0.16 -0.6 421,626 - BWI76_RS02045 0.25 -1.8 421,950 + BWI76_RS02045 0.62 -0.5 422,000 + BWI76_RS02045 0.67 -2.5 422,148 + BWI76_RS02045 0.84 +0.2 422,161 - BWI76_RS02045 0.85 -1.0 422,308 + -0.2 422,316 - -0.2 422,316 - -2.4 422,355 + +3.3 422,355 + -0.4 422,355 + -0.8 422,355 + -0.1 422,355 + -0.9 422,355 + -1.1 422,355 + +2.2 422,356 + -1.8 422,356 + -0.6 422,363 - +1.7 422,363 - -0.5 422,363 - -0.5 422,363 - -1.6 422,363 - -0.3 422,363 - +0.4 422,363 - -0.4 422,363 - +0.2 422,368 - -0.4 422,415 - +1.6 422,418 + +0.8 422,429 + -0.8 422,429 + -0.3 422,437 - -0.5 422,461 + BWI76_RS02050 0.15 +0.3 422,496 + BWI76_RS02050 0.24 -0.3 422,782 + -0.1 422,792 + +1.4 422,792 + -0.8 422,792 + -0.7 422,816 - -0.5 422,978 - BWI76_RS02055 0.26 +0.4 423,081 + BWI76_RS02055 0.41 -0.2 423,182 + BWI76_RS02055 0.55 -0.8 423,271 - BWI76_RS02055 0.68 +0.2
Or see this region's nucleotide sequence