Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01080

Experiment: KLEB30 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01075 and BWI76_RS01080 are separated by 242 nucleotidesBWI76_RS01080 and BWI76_RS01085 are separated by 21 nucleotides BWI76_RS01075: BWI76_RS01075 - transcription termination factor Rho, at 220,712 to 221,971 _RS01075 BWI76_RS01080: BWI76_RS01080 - undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 222,214 to 223,308 _RS01080 BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376 _RS01085 Position (kb) 222 223 224Strain fitness (log2 ratio) -1 0 1 2 3 4at 222.063 kb on + strandat 222.063 kb on + strandat 222.071 kb on - strandat 222.071 kb on - strandat 222.073 kb on - strandat 222.081 kb on - strandat 222.081 kb on - strandat 222.137 kb on + strandat 222.142 kb on + strandat 222.145 kb on - strandat 222.157 kb on + strandat 222.159 kb on - strandat 222.171 kb on + strandat 222.171 kb on + strandat 222.179 kb on - strandat 222.179 kb on - strandat 222.179 kb on - strandat 222.179 kb on - strandat 222.179 kb on - strandat 222.179 kb on - strandat 222.196 kb on + strandat 222.196 kb on + strandat 222.201 kb on + strandat 222.203 kb on + strandat 222.224 kb on - strandat 222.270 kb on + strandat 222.310 kb on - strandat 222.310 kb on - strandat 222.345 kb on - strand, within BWI76_RS01080at 222.353 kb on - strand, within BWI76_RS01080at 222.434 kb on + strand, within BWI76_RS01080at 222.435 kb on + strand, within BWI76_RS01080at 222.439 kb on + strand, within BWI76_RS01080at 222.441 kb on - strand, within BWI76_RS01080at 222.441 kb on - strand, within BWI76_RS01080at 222.447 kb on - strand, within BWI76_RS01080at 222.447 kb on - strand, within BWI76_RS01080at 222.449 kb on + strand, within BWI76_RS01080at 222.496 kb on - strand, within BWI76_RS01080at 222.612 kb on - strand, within BWI76_RS01080at 222.629 kb on + strand, within BWI76_RS01080at 222.647 kb on - strand, within BWI76_RS01080at 222.703 kb on + strand, within BWI76_RS01080at 222.762 kb on - strand, within BWI76_RS01080at 222.842 kb on + strand, within BWI76_RS01080at 222.921 kb on + strand, within BWI76_RS01080at 222.935 kb on + strand, within BWI76_RS01080at 223.009 kb on - strand, within BWI76_RS01080at 223.086 kb on + strand, within BWI76_RS01080at 223.116 kb on - strand, within BWI76_RS01080at 223.133 kb on + strand, within BWI76_RS01080at 223.238 kb on + strandat 223.261 kb on - strandat 223.301 kb on - strandat 223.325 kb on - strandat 223.326 kb on + strandat 223.334 kb on - strandat 223.419 kb on + strandat 223.438 kb on + strand, within BWI76_RS01085at 223.608 kb on + strand, within BWI76_RS01085at 223.697 kb on + strand, within BWI76_RS01085at 223.917 kb on + strand, within BWI76_RS01085at 224.006 kb on + strand, within BWI76_RS01085at 224.069 kb on + strand, within BWI76_RS01085at 224.097 kb on + strand, within BWI76_RS01085at 224.119 kb on - strand, within BWI76_RS01085at 224.184 kb on - strand, within BWI76_RS01085

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB30 MOI 1
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222,063 + -0.6
222,063 + +0.1
222,071 - -1.6
222,071 - -1.1
222,073 - -0.7
222,081 - -0.5
222,081 - +1.6
222,137 + -0.2
222,142 + +0.2
222,145 - +2.2
222,157 + +1.3
222,159 - +2.8
222,171 + +2.3
222,171 + +2.3
222,179 - +3.8
222,179 - +1.9
222,179 - +3.0
222,179 - +3.4
222,179 - +2.8
222,179 - +3.1
222,196 + +2.9
222,196 + +1.8
222,201 + +0.3
222,203 + +1.5
222,224 - +3.2
222,270 + +1.5
222,310 - +3.4
222,310 - -1.0
222,345 - BWI76_RS01080 0.12 +2.7
222,353 - BWI76_RS01080 0.13 +4.1
222,434 + BWI76_RS01080 0.20 +3.8
222,435 + BWI76_RS01080 0.20 +2.0
222,439 + BWI76_RS01080 0.21 +4.5
222,441 - BWI76_RS01080 0.21 -0.3
222,441 - BWI76_RS01080 0.21 +4.5
222,447 - BWI76_RS01080 0.21 +2.9
222,447 - BWI76_RS01080 0.21 +2.4
222,449 + BWI76_RS01080 0.21 +3.5
222,496 - BWI76_RS01080 0.26 +4.3
222,612 - BWI76_RS01080 0.36 +2.7
222,629 + BWI76_RS01080 0.38 +1.4
222,647 - BWI76_RS01080 0.40 +3.3
222,703 + BWI76_RS01080 0.45 +2.6
222,762 - BWI76_RS01080 0.50 +3.0
222,842 + BWI76_RS01080 0.57 +3.4
222,921 + BWI76_RS01080 0.65 +2.7
222,935 + BWI76_RS01080 0.66 +3.9
223,009 - BWI76_RS01080 0.73 +4.3
223,086 + BWI76_RS01080 0.80 +3.4
223,116 - BWI76_RS01080 0.82 +2.5
223,133 + BWI76_RS01080 0.84 +3.9
223,238 + +2.1
223,261 - +3.5
223,301 - +3.7
223,325 - +2.5
223,326 + -0.5
223,334 - +2.9
223,419 + +1.5
223,438 + BWI76_RS01085 0.10 +0.7
223,608 + BWI76_RS01085 0.27 -0.0
223,697 + BWI76_RS01085 0.35 +0.9
223,917 + BWI76_RS01085 0.56 +0.8
224,006 + BWI76_RS01085 0.65 +0.2
224,069 + BWI76_RS01085 0.71 -1.3
224,097 + BWI76_RS01085 0.73 -1.5
224,119 - BWI76_RS01085 0.75 +2.8
224,184 - BWI76_RS01085 0.82 +2.3

Or see this region's nucleotide sequence