Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06955 and BWI76_RS06960 are separated by 70 nucleotides BWI76_RS06960 and BWI76_RS06965 are separated by 209 nucleotides BWI76_RS06965 and BWI76_RS06970 are separated by 106 nucleotides
BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050
_RS06955
BWI76_RS06960: BWI76_RS06960 - monooxygenase, at 1,462,121 to 1,462,426
_RS06960
BWI76_RS06965: BWI76_RS06965 - membrane protein, at 1,462,636 to 1,463,781
_RS06965
BWI76_RS06970: BWI76_RS06970 - selenophosphate-dependent tRNA 2-selenouridine synthase, at 1,463,888 to 1,464,973
_RS06970
Position (kb)
1462
1463
1464 Strain fitness (log2 ratio)
-2
-1
0
1 at 1461.869 kb on + strand at 1462.278 kb on + strand, within BWI76_RS06960 at 1462.692 kb on - strand at 1462.801 kb on + strand, within BWI76_RS06965 at 1462.891 kb on - strand, within BWI76_RS06965 at 1462.938 kb on - strand, within BWI76_RS06965 at 1462.991 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965 at 1463.061 kb on - strand, within BWI76_RS06965 at 1463.422 kb on + strand, within BWI76_RS06965 at 1463.685 kb on + strand at 1463.775 kb on + strand at 1463.810 kb on + strand at 1463.873 kb on - strand at 1463.889 kb on - strand at 1464.424 kb on + strand, within BWI76_RS06970 at 1464.550 kb on - strand, within BWI76_RS06970 at 1464.601 kb on + strand, within BWI76_RS06970 at 1464.622 kb on - strand, within BWI76_RS06970 at 1464.637 kb on - strand, within BWI76_RS06970
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 1,461,869 + -0.2 1,462,278 + BWI76_RS06960 0.51 +0.3 1,462,692 - -0.4 1,462,801 + BWI76_RS06965 0.14 -1.5 1,462,891 - BWI76_RS06965 0.22 -0.4 1,462,938 - BWI76_RS06965 0.26 -1.8 1,462,991 - BWI76_RS06965 0.31 -0.0 1,463,012 - BWI76_RS06965 0.33 -0.4 1,463,012 - BWI76_RS06965 0.33 +0.1 1,463,061 - BWI76_RS06965 0.37 -1.0 1,463,422 + BWI76_RS06965 0.69 -0.7 1,463,685 + +0.7 1,463,775 + +0.3 1,463,810 + -0.7 1,463,873 - -0.7 1,463,889 - -0.4 1,464,424 + BWI76_RS06970 0.49 +0.2 1,464,550 - BWI76_RS06970 0.61 -0.0 1,464,601 + BWI76_RS06970 0.66 -0.3 1,464,622 - BWI76_RS06970 0.68 -0.3 1,464,637 - BWI76_RS06970 0.69 +1.5
Or see this region's nucleotide sequence