Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27135 and BWI76_RS27140 overlap by 4 nucleotides BWI76_RS27140 and BWI76_RS27145 are separated by 95 nucleotides BWI76_RS27145 and BWI76_RS27150 are separated by 91 nucleotides
BWI76_RS27135: BWI76_RS27135 - selenocysteine-specific translation factor, at 5,631,943 to 5,633,784
_RS27135
BWI76_RS27140: BWI76_RS27140 - L-selenocysteinyl-tRNA(Sec) synthase, at 5,633,781 to 5,635,169
_RS27140
BWI76_RS27145: BWI76_RS27145 - glutathione S-transferase, at 5,635,265 to 5,635,873
_RS27145
BWI76_RS27150: BWI76_RS27150 - secretion protein HlyD, at 5,635,965 to 5,637,101
_RS27150
Position (kb)
5633
5634
5635
5636 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5632.888 kb on - strand, within BWI76_RS27135 at 5632.994 kb on - strand, within BWI76_RS27135 at 5633.113 kb on - strand, within BWI76_RS27135 at 5633.251 kb on + strand, within BWI76_RS27135 at 5633.276 kb on + strand, within BWI76_RS27135 at 5633.278 kb on - strand, within BWI76_RS27135 at 5633.325 kb on + strand, within BWI76_RS27135 at 5633.326 kb on + strand, within BWI76_RS27135 at 5633.356 kb on - strand, within BWI76_RS27135 at 5633.380 kb on - strand, within BWI76_RS27135 at 5633.401 kb on - strand, within BWI76_RS27135 at 5633.414 kb on + strand, within BWI76_RS27135 at 5633.657 kb on + strand at 5633.824 kb on - strand at 5633.829 kb on - strand at 5633.831 kb on + strand at 5633.834 kb on - strand at 5633.834 kb on - strand at 5633.836 kb on + strand at 5633.890 kb on - strand at 5633.936 kb on + strand, within BWI76_RS27140 at 5633.943 kb on + strand, within BWI76_RS27140 at 5633.946 kb on - strand, within BWI76_RS27140 at 5634.120 kb on - strand, within BWI76_RS27140 at 5634.163 kb on + strand, within BWI76_RS27140 at 5634.274 kb on - strand, within BWI76_RS27140 at 5634.360 kb on + strand, within BWI76_RS27140 at 5634.490 kb on - strand, within BWI76_RS27140 at 5634.586 kb on + strand, within BWI76_RS27140 at 5634.904 kb on + strand, within BWI76_RS27140 at 5634.991 kb on - strand, within BWI76_RS27140 at 5635.063 kb on + strand at 5635.288 kb on + strand at 5635.424 kb on - strand, within BWI76_RS27145 at 5635.426 kb on + strand, within BWI76_RS27145 at 5635.434 kb on - strand, within BWI76_RS27145 at 5635.434 kb on - strand, within BWI76_RS27145 at 5635.468 kb on + strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.617 kb on + strand, within BWI76_RS27145 at 5635.625 kb on - strand, within BWI76_RS27145 at 5635.660 kb on + strand, within BWI76_RS27145 at 5635.668 kb on - strand, within BWI76_RS27145 at 5635.668 kb on - strand, within BWI76_RS27145 at 5635.750 kb on + strand, within BWI76_RS27145 at 5635.758 kb on + strand, within BWI76_RS27145 at 5635.897 kb on + strand at 5635.899 kb on + strand at 5635.944 kb on + strand at 5636.107 kb on + strand, within BWI76_RS27150 at 5636.114 kb on + strand, within BWI76_RS27150 at 5636.114 kb on + strand, within BWI76_RS27150 at 5636.155 kb on - strand, within BWI76_RS27150
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 5,632,888 - BWI76_RS27135 0.51 -1.6 5,632,994 - BWI76_RS27135 0.57 -1.2 5,633,113 - BWI76_RS27135 0.64 +0.8 5,633,251 + BWI76_RS27135 0.71 -0.3 5,633,276 + BWI76_RS27135 0.72 +1.3 5,633,278 - BWI76_RS27135 0.72 -0.1 5,633,325 + BWI76_RS27135 0.75 -1.9 5,633,326 + BWI76_RS27135 0.75 +1.8 5,633,356 - BWI76_RS27135 0.77 -1.1 5,633,380 - BWI76_RS27135 0.78 +0.7 5,633,401 - BWI76_RS27135 0.79 +0.3 5,633,414 + BWI76_RS27135 0.80 +0.4 5,633,657 + +1.3 5,633,824 - +1.1 5,633,829 - -0.4 5,633,831 + +0.0 5,633,834 - -0.3 5,633,834 - -0.4 5,633,836 + -0.3 5,633,890 - +0.4 5,633,936 + BWI76_RS27140 0.11 +0.9 5,633,943 + BWI76_RS27140 0.12 -1.0 5,633,946 - BWI76_RS27140 0.12 -0.1 5,634,120 - BWI76_RS27140 0.24 -1.0 5,634,163 + BWI76_RS27140 0.28 -0.8 5,634,274 - BWI76_RS27140 0.35 -0.1 5,634,360 + BWI76_RS27140 0.42 -2.6 5,634,490 - BWI76_RS27140 0.51 -0.5 5,634,586 + BWI76_RS27140 0.58 -0.8 5,634,904 + BWI76_RS27140 0.81 -0.8 5,634,991 - BWI76_RS27140 0.87 -1.0 5,635,063 + -1.4 5,635,288 + +0.3 5,635,424 - BWI76_RS27145 0.26 +0.5 5,635,426 + BWI76_RS27145 0.26 -1.1 5,635,434 - BWI76_RS27145 0.28 -0.6 5,635,434 - BWI76_RS27145 0.28 -0.1 5,635,468 + BWI76_RS27145 0.33 +1.4 5,635,476 - BWI76_RS27145 0.35 +0.2 5,635,476 - BWI76_RS27145 0.35 +1.6 5,635,476 - BWI76_RS27145 0.35 +0.1 5,635,617 + BWI76_RS27145 0.58 +0.5 5,635,625 - BWI76_RS27145 0.59 -1.2 5,635,660 + BWI76_RS27145 0.65 +0.4 5,635,668 - BWI76_RS27145 0.66 +0.4 5,635,668 - BWI76_RS27145 0.66 -0.1 5,635,750 + BWI76_RS27145 0.80 -0.5 5,635,758 + BWI76_RS27145 0.81 +0.2 5,635,897 + -0.7 5,635,899 + -0.1 5,635,944 + +1.8 5,636,107 + BWI76_RS27150 0.12 -0.6 5,636,114 + BWI76_RS27150 0.13 -0.6 5,636,114 + BWI76_RS27150 0.13 -1.5 5,636,155 - BWI76_RS27150 0.17 +1.5
Or see this region's nucleotide sequence