Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25370 and BWI76_RS25375 are separated by 26 nucleotides BWI76_RS25375 and BWI76_RS25380 are separated by 126 nucleotides BWI76_RS25380 and BWI76_RS25385 are separated by 15 nucleotides
BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441
_RS25370
BWI76_RS25375: BWI76_RS25375 - hypothetical protein, at 5,274,468 to 5,275,166
_RS25375
BWI76_RS25380: BWI76_RS25380 - oxaloacetate decarboxylase subunit beta, at 5,275,293 to 5,276,594
_RS25380
BWI76_RS25385: BWI76_RS25385 - oxaloacetate decarboxylase subunit alpha, at 5,276,610 to 5,278,376
_RS25385
Position (kb) 5275
5276
5277 Strain fitness (log2 ratio) -2
-1
0
1 at 5274.451 kb on + strand at 5274.490 kb on + strand at 5274.574 kb on - strand, within BWI76_RS25375 at 5274.606 kb on + strand at 5274.825 kb on - strand, within BWI76_RS25375 at 5275.217 kb on + strand at 5275.293 kb on - strand at 5275.946 kb on - strand, within BWI76_RS25380 at 5276.558 kb on + strand at 5276.622 kb on + strand at 5276.924 kb on - strand, within BWI76_RS25385 at 5277.076 kb on - strand, within BWI76_RS25385 at 5277.088 kb on - strand, within BWI76_RS25385
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 5,274,451 + -0.3 5,274,490 + -1.0 5,274,574 - BWI76_RS25375 0.15 +0.3 5,274,606 + -2.1 5,274,825 - BWI76_RS25375 0.51 -0.7 5,275,217 + +0.9 5,275,293 - -0.5 5,275,946 - BWI76_RS25380 0.50 -1.0 5,276,558 + -0.4 5,276,622 + -0.5 5,276,924 - BWI76_RS25385 0.18 +0.3 5,277,076 - BWI76_RS25385 0.26 -0.3 5,277,088 - BWI76_RS25385 0.27 -1.1
Or see this region's nucleotide sequence