Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19565 and BWI76_RS19570 are separated by 200 nucleotides BWI76_RS19570 and BWI76_RS19575 are separated by 8 nucleotides BWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotides
BWI76_RS19565: BWI76_RS19565 - tRNA dihydrouridine(16) synthase DusC, at 4,090,654 to 4,091,592
_RS19565
BWI76_RS19570: BWI76_RS19570 - 3-hydroxybenzoate 6-hydroxylase, at 4,091,793 to 4,092,986
_RS19570
BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639
_RS19575
BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346
_RS19580
Position (kb) 4091
4092
4093 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4090.856 kb on - strand, within BWI76_RS19565 at 4090.858 kb on + strand, within BWI76_RS19565 at 4090.906 kb on + strand, within BWI76_RS19565 at 4091.197 kb on + strand, within BWI76_RS19565 at 4091.311 kb on - strand, within BWI76_RS19565 at 4091.436 kb on + strand, within BWI76_RS19565 at 4091.444 kb on - strand, within BWI76_RS19565 at 4091.636 kb on + strand at 4091.799 kb on - strand at 4091.872 kb on - strand at 4091.988 kb on - strand, within BWI76_RS19570 at 4091.990 kb on - strand, within BWI76_RS19570 at 4092.031 kb on + strand, within BWI76_RS19570 at 4092.062 kb on - strand, within BWI76_RS19570 at 4092.084 kb on + strand, within BWI76_RS19570 at 4092.087 kb on - strand, within BWI76_RS19570 at 4092.705 kb on + strand, within BWI76_RS19570 at 4092.977 kb on + strand at 4093.080 kb on - strand, within BWI76_RS19575 at 4093.158 kb on - strand, within BWI76_RS19575 at 4093.886 kb on - strand, within BWI76_RS19580
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 4,090,856 - BWI76_RS19565 0.22 +0.1 4,090,858 + BWI76_RS19565 0.22 -0.4 4,090,906 + BWI76_RS19565 0.27 -1.9 4,091,197 + BWI76_RS19565 0.58 +0.9 4,091,311 - BWI76_RS19565 0.70 -0.2 4,091,436 + BWI76_RS19565 0.83 +0.1 4,091,444 - BWI76_RS19565 0.84 -0.5 4,091,636 + -0.7 4,091,799 - +0.5 4,091,872 - -0.5 4,091,988 - BWI76_RS19570 0.16 +2.1 4,091,990 - BWI76_RS19570 0.16 +0.1 4,092,031 + BWI76_RS19570 0.20 +1.0 4,092,062 - BWI76_RS19570 0.23 +1.6 4,092,084 + BWI76_RS19570 0.24 +0.6 4,092,087 - BWI76_RS19570 0.25 +0.5 4,092,705 + BWI76_RS19570 0.76 -0.2 4,092,977 + -0.5 4,093,080 - BWI76_RS19575 0.13 +0.3 4,093,158 - BWI76_RS19575 0.25 +1.2 4,093,886 - BWI76_RS19580 0.34 -0.5
Or see this region's nucleotide sequence