Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03390 and BWI76_RS03395 are separated by 165 nucleotides BWI76_RS03395 and BWI76_RS03400 are separated by 146 nucleotides BWI76_RS03400 and BWI76_RS03405 are separated by 289 nucleotides
BWI76_RS03390: BWI76_RS03390 - hypothetical protein, at 698,544 to 699,287
_RS03390
BWI76_RS03395: BWI76_RS03395 - glyoxalase family protein, at 699,453 to 699,887
_RS03395
BWI76_RS03400: BWI76_RS03400 - hypothetical protein, at 700,034 to 700,357
_RS03400
BWI76_RS03405: BWI76_RS03405 - DNA/RNA non-specific endonuclease, at 700,647 to 701,351
_RS03405
Position (kb) 700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 699.054 kb on + strand, within BWI76_RS03390 at 699.054 kb on + strand, within BWI76_RS03390 at 699.071 kb on + strand, within BWI76_RS03390 at 699.072 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.088 kb on + strand, within BWI76_RS03390 at 699.102 kb on - strand, within BWI76_RS03390 at 699.120 kb on - strand, within BWI76_RS03390 at 699.125 kb on + strand, within BWI76_RS03390 at 699.133 kb on - strand, within BWI76_RS03390 at 699.186 kb on + strand, within BWI76_RS03390 at 699.186 kb on + strand, within BWI76_RS03390 at 699.194 kb on - strand, within BWI76_RS03390 at 699.280 kb on - strand at 699.343 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.385 kb on - strand at 699.385 kb on - strand at 699.390 kb on - strand at 699.390 kb on - strand at 699.392 kb on + strand at 699.394 kb on + strand at 699.394 kb on + strand at 699.400 kb on - strand at 699.400 kb on - strand at 699.402 kb on - strand at 699.402 kb on - strand at 699.404 kb on + strand at 699.404 kb on + strand at 699.405 kb on + strand at 699.405 kb on + strand at 699.405 kb on + strand at 699.412 kb on - strand at 699.417 kb on - strand at 699.419 kb on + strand at 699.427 kb on - strand at 699.429 kb on + strand at 699.432 kb on - strand at 699.435 kb on + strand at 699.436 kb on + strand at 699.443 kb on - strand at 699.443 kb on - strand at 699.443 kb on - strand at 699.444 kb on + strand at 699.444 kb on + strand at 699.452 kb on - strand at 699.452 kb on - strand at 699.458 kb on + strand at 699.458 kb on + strand at 699.460 kb on - strand at 699.466 kb on - strand at 699.476 kb on - strand at 699.476 kb on - strand at 699.479 kb on + strand at 699.479 kb on + strand at 699.481 kb on + strand at 699.481 kb on + strand at 699.484 kb on + strand at 699.487 kb on - strand at 699.489 kb on + strand at 699.497 kb on - strand, within BWI76_RS03395 at 699.497 kb on - strand, within BWI76_RS03395 at 699.503 kb on - strand, within BWI76_RS03395 at 699.512 kb on - strand, within BWI76_RS03395 at 699.515 kb on + strand, within BWI76_RS03395 at 699.553 kb on + strand, within BWI76_RS03395 at 699.561 kb on + strand, within BWI76_RS03395 at 699.579 kb on + strand, within BWI76_RS03395 at 699.601 kb on + strand, within BWI76_RS03395 at 699.609 kb on - strand, within BWI76_RS03395 at 699.609 kb on - strand, within BWI76_RS03395 at 699.634 kb on - strand, within BWI76_RS03395 at 699.675 kb on + strand, within BWI76_RS03395 at 699.682 kb on + strand, within BWI76_RS03395 at 699.687 kb on + strand, within BWI76_RS03395 at 699.690 kb on - strand, within BWI76_RS03395 at 699.695 kb on + strand, within BWI76_RS03395 at 699.717 kb on + strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.774 kb on + strand, within BWI76_RS03395 at 699.838 kb on + strand, within BWI76_RS03395 at 699.846 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.880 kb on + strand at 699.884 kb on + strand at 699.892 kb on - strand at 699.926 kb on - strand at 699.935 kb on + strand at 699.952 kb on - strand at 699.961 kb on + strand at 699.966 kb on + strand at 699.969 kb on - strand at 699.969 kb on - strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.974 kb on - strand at 699.974 kb on - strand at 699.982 kb on + strand at 699.982 kb on + strand at 699.998 kb on - strand at 700.026 kb on - strand at 700.031 kb on - strand at 700.032 kb on + strand at 700.032 kb on + strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.058 kb on + strand at 700.073 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.112 kb on - strand, within BWI76_RS03400 at 700.132 kb on + strand, within BWI76_RS03400 at 700.153 kb on + strand, within BWI76_RS03400 at 700.158 kb on + strand, within BWI76_RS03400 at 700.166 kb on - strand, within BWI76_RS03400 at 700.168 kb on + strand at 700.171 kb on - strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.205 kb on - strand, within BWI76_RS03400 at 700.213 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.235 kb on + strand, within BWI76_RS03400 at 700.259 kb on - strand, within BWI76_RS03400 at 700.263 kb on + strand, within BWI76_RS03400 at 700.269 kb on + strand, within BWI76_RS03400 at 700.277 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.292 kb on - strand, within BWI76_RS03400 at 700.315 kb on + strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.333 kb on - strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.352 kb on + strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.360 kb on - strand at 700.377 kb on + strand at 700.390 kb on - strand at 700.395 kb on + strand at 700.395 kb on + strand at 700.397 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.424 kb on - strand at 700.432 kb on - strand at 700.437 kb on + strand at 700.438 kb on + strand at 700.445 kb on - strand at 700.446 kb on - strand at 700.446 kb on - strand at 700.472 kb on + strand at 700.503 kb on - strand at 700.535 kb on + strand at 700.593 kb on - strand at 700.595 kb on + strand at 700.628 kb on + strand at 700.629 kb on + strand at 700.635 kb on + strand at 700.636 kb on + strand at 700.636 kb on - strand at 700.641 kb on + strand at 700.644 kb on - strand at 700.656 kb on + strand at 700.657 kb on + strand at 700.659 kb on - strand at 700.664 kb on - strand at 700.671 kb on - strand at 700.675 kb on + strand at 700.695 kb on - strand at 700.701 kb on + strand at 700.709 kb on - strand at 700.709 kb on - strand at 700.716 kb on - strand at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.722 kb on + strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.736 kb on - strand, within BWI76_RS03405 at 700.749 kb on - strand, within BWI76_RS03405 at 700.763 kb on + strand, within BWI76_RS03405 at 700.771 kb on - strand, within BWI76_RS03405 at 700.844 kb on + strand, within BWI76_RS03405 at 700.887 kb on + strand, within BWI76_RS03405 at 700.900 kb on - strand, within BWI76_RS03405 at 700.929 kb on - strand, within BWI76_RS03405 at 701.005 kb on - strand, within BWI76_RS03405 at 701.042 kb on + strand, within BWI76_RS03405 at 701.086 kb on + strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.224 kb on + strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.242 kb on - strand, within BWI76_RS03405 at 701.272 kb on + strand, within BWI76_RS03405 at 701.280 kb on - strand, within BWI76_RS03405 at 701.297 kb on + strand at 701.298 kb on - strand at 701.305 kb on - strand at 701.306 kb on - strand at 701.308 kb on + strand at 701.318 kb on + strand at 701.326 kb on - strand at 701.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 699,054 + BWI76_RS03390 0.69 -2.4 699,054 + BWI76_RS03390 0.69 -1.1 699,071 + BWI76_RS03390 0.71 -1.3 699,072 - BWI76_RS03390 0.71 +1.0 699,082 - BWI76_RS03390 0.72 -0.0 699,082 - BWI76_RS03390 0.72 -2.0 699,082 - BWI76_RS03390 0.72 +0.4 699,088 + BWI76_RS03390 0.73 -0.6 699,102 - BWI76_RS03390 0.75 -0.2 699,120 - BWI76_RS03390 0.77 +0.4 699,125 + BWI76_RS03390 0.78 -0.1 699,133 - BWI76_RS03390 0.79 +2.9 699,186 + BWI76_RS03390 0.86 -0.4 699,186 + BWI76_RS03390 0.86 -0.1 699,194 - BWI76_RS03390 0.87 -0.4 699,280 - -0.0 699,343 + -1.0 699,382 + -0.4 699,382 + +0.5 699,382 + -0.2 699,382 + -0.8 699,385 - -0.2 699,385 - +0.5 699,390 - -1.3 699,390 - -0.1 699,392 + +1.9 699,394 + +0.4 699,394 + -0.1 699,400 - -0.2 699,400 - -0.7 699,402 - +0.6 699,402 - -0.3 699,404 + +1.2 699,404 + -2.0 699,405 + -0.4 699,405 + -0.1 699,405 + -0.4 699,412 - -0.8 699,417 - +0.2 699,419 + -0.5 699,427 - -0.0 699,429 + +1.3 699,432 - -0.0 699,435 + -1.7 699,436 + +0.1 699,443 - -0.6 699,443 - +0.2 699,443 - -0.4 699,444 + -0.8 699,444 + +0.1 699,452 - -1.8 699,452 - -0.6 699,458 + -0.2 699,458 + -0.5 699,460 - -0.5 699,466 - -0.3 699,476 - +0.4 699,476 - -0.3 699,479 + -1.0 699,479 + -1.1 699,481 + -0.7 699,481 + -1.4 699,484 + -0.0 699,487 - -0.9 699,489 + -0.1 699,497 - BWI76_RS03395 0.10 -1.8 699,497 - BWI76_RS03395 0.10 +0.5 699,503 - BWI76_RS03395 0.11 +0.1 699,512 - BWI76_RS03395 0.14 -0.0 699,515 + BWI76_RS03395 0.14 +0.4 699,553 + BWI76_RS03395 0.23 -0.8 699,561 + BWI76_RS03395 0.25 +0.5 699,579 + BWI76_RS03395 0.29 +0.9 699,601 + BWI76_RS03395 0.34 +0.4 699,609 - BWI76_RS03395 0.36 +0.5 699,609 - BWI76_RS03395 0.36 -0.8 699,634 - BWI76_RS03395 0.42 +0.9 699,675 + BWI76_RS03395 0.51 -0.6 699,682 + BWI76_RS03395 0.53 +0.4 699,687 + BWI76_RS03395 0.54 -1.7 699,690 - BWI76_RS03395 0.54 +0.3 699,695 + BWI76_RS03395 0.56 -0.0 699,717 + BWI76_RS03395 0.61 -0.7 699,747 - BWI76_RS03395 0.68 +0.4 699,747 - BWI76_RS03395 0.68 -0.4 699,770 - BWI76_RS03395 0.73 -0.1 699,770 - BWI76_RS03395 0.73 +0.4 699,774 + BWI76_RS03395 0.74 -0.2 699,838 + BWI76_RS03395 0.89 -0.9 699,846 - -0.1 699,850 - -0.9 699,850 - +0.0 699,850 - -1.6 699,850 - -0.2 699,880 + -0.7 699,884 + -0.1 699,892 - -0.4 699,926 - -3.0 699,935 + -0.2 699,952 - -0.2 699,961 + -0.2 699,966 + -0.2 699,969 - -1.1 699,969 - -0.4 699,971 + +0.0 699,971 + -0.4 699,971 + +1.1 699,974 - +1.5 699,974 - -0.9 699,982 + +1.3 699,982 + +0.1 699,998 - +0.5 700,026 - -0.1 700,031 - -1.3 700,032 + +0.3 700,032 + +2.8 700,040 - +0.3 700,040 - +0.5 700,040 - -0.6 700,058 + +0.2 700,073 - BWI76_RS03400 0.12 -0.3 700,107 - BWI76_RS03400 0.23 -0.2 700,107 - BWI76_RS03400 0.23 +0.4 700,112 - BWI76_RS03400 0.24 -2.7 700,132 + BWI76_RS03400 0.30 -0.7 700,153 + BWI76_RS03400 0.37 -1.0 700,158 + BWI76_RS03400 0.38 -1.5 700,166 - BWI76_RS03400 0.41 +0.5 700,168 + +0.3 700,171 - BWI76_RS03400 0.42 -0.2 700,186 + BWI76_RS03400 0.47 -0.5 700,186 + BWI76_RS03400 0.47 +0.4 700,194 - BWI76_RS03400 0.49 +0.3 700,194 - BWI76_RS03400 0.49 -0.6 700,194 - BWI76_RS03400 0.49 +0.1 700,205 - BWI76_RS03400 0.53 -0.1 700,213 - BWI76_RS03400 0.55 -0.5 700,227 - BWI76_RS03400 0.60 -1.7 700,227 - BWI76_RS03400 0.60 -0.4 700,235 + BWI76_RS03400 0.62 -1.1 700,259 - BWI76_RS03400 0.69 -0.2 700,263 + BWI76_RS03400 0.71 -1.5 700,269 + BWI76_RS03400 0.73 -0.6 700,277 - BWI76_RS03400 0.75 -2.3 700,283 - BWI76_RS03400 0.77 +0.4 700,283 - BWI76_RS03400 0.77 -0.6 700,292 - BWI76_RS03400 0.80 -0.0 700,315 + BWI76_RS03400 0.87 +0.2 700,323 - BWI76_RS03400 0.89 -1.7 700,323 - BWI76_RS03400 0.89 -0.2 700,333 - -0.5 700,347 + -0.7 700,347 + -0.4 700,347 + -0.6 700,347 + -0.6 700,352 + +0.2 700,355 - +0.1 700,355 - -0.4 700,355 - -2.3 700,355 - -0.2 700,355 - -0.6 700,360 - -0.7 700,377 + -0.3 700,390 - +0.1 700,395 + +0.8 700,395 + -2.3 700,397 + +0.5 700,414 + +1.3 700,414 + +1.8 700,414 + -0.9 700,422 - -0.5 700,422 - -0.7 700,422 - -0.3 700,422 - -0.2 700,422 - -1.3 700,422 - +1.1 700,422 - -0.0 700,422 - -1.4 700,422 - -1.8 700,422 - +0.9 700,422 - +0.1 700,424 - -0.2 700,432 - +0.6 700,437 + +0.3 700,438 + -2.3 700,445 - -0.7 700,446 - -2.5 700,446 - +0.7 700,472 + +0.4 700,503 - +1.5 700,535 + +2.8 700,593 - -1.1 700,595 + -0.7 700,628 + +0.6 700,629 + +0.1 700,635 + -0.1 700,636 + -1.3 700,636 - +2.8 700,641 + +0.5 700,644 - -0.6 700,656 + -1.3 700,657 + -0.7 700,659 - -0.5 700,664 - +1.5 700,671 - -0.1 700,675 + -0.4 700,695 - +0.1 700,701 + +0.8 700,709 - +1.2 700,709 - +0.6 700,716 - -0.4 700,718 + BWI76_RS03405 0.10 -1.4 700,718 + BWI76_RS03405 0.10 -0.2 700,718 + BWI76_RS03405 0.10 +0.3 700,718 + BWI76_RS03405 0.10 -0.1 700,722 + BWI76_RS03405 0.11 +0.8 700,726 - BWI76_RS03405 0.11 -0.4 700,726 - BWI76_RS03405 0.11 +0.1 700,736 - BWI76_RS03405 0.13 -0.2 700,749 - BWI76_RS03405 0.14 +1.7 700,763 + BWI76_RS03405 0.16 -0.8 700,771 - BWI76_RS03405 0.18 +0.2 700,844 + BWI76_RS03405 0.28 +0.2 700,887 + BWI76_RS03405 0.34 +0.1 700,900 - BWI76_RS03405 0.36 -2.7 700,929 - BWI76_RS03405 0.40 +0.1 701,005 - BWI76_RS03405 0.51 -0.4 701,042 + BWI76_RS03405 0.56 -0.4 701,086 + BWI76_RS03405 0.62 -0.6 701,212 - BWI76_RS03405 0.80 -0.1 701,212 - BWI76_RS03405 0.80 -0.7 701,224 + BWI76_RS03405 0.82 -0.1 701,237 - BWI76_RS03405 0.84 +0.1 701,237 - BWI76_RS03405 0.84 +0.9 701,242 - BWI76_RS03405 0.84 -0.1 701,272 + BWI76_RS03405 0.89 -0.8 701,280 - BWI76_RS03405 0.90 -0.7 701,297 + -0.0 701,298 - -0.9 701,305 - +3.3 701,306 - +0.5 701,308 + -0.6 701,318 + +1.4 701,326 - +0.1 701,349 - -0.6
Or see this region's nucleotide sequence