Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03140 and BWI76_RS03145 are separated by 233 nucleotides BWI76_RS03145 and BWI76_RS03150 are separated by 164 nucleotides BWI76_RS03150 and BWI76_RS03155 are separated by 126 nucleotides
BWI76_RS03140: BWI76_RS03140 - alcohol dehydrogenase, at 647,173 to 648,192
_RS03140
BWI76_RS03145: BWI76_RS03145 - tRNA-Leu, at 648,426 to 648,510
_RS03145
BWI76_RS03150: BWI76_RS03150 - site-specific recombinase, phage integrase family protein, at 648,675 to 649,937
_RS03150
BWI76_RS03155: BWI76_RS03155 - hypothetical protein, at 650,064 to 650,966
_RS03155
Position (kb) 648
649
650 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 647.891 kb on + strand, within BWI76_RS03140 at 647.899 kb on - strand, within BWI76_RS03140 at 647.945 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 648.090 kb on - strand, within BWI76_RS03140 at 648.195 kb on - strand at 648.217 kb on + strand at 648.237 kb on - strand at 648.241 kb on + strand at 648.285 kb on - strand at 648.288 kb on - strand at 648.309 kb on - strand at 648.685 kb on + strand at 648.691 kb on + strand at 648.695 kb on + strand at 648.703 kb on - strand at 648.710 kb on + strand at 648.841 kb on + strand, within BWI76_RS03150 at 648.867 kb on - strand, within BWI76_RS03150 at 648.869 kb on + strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.913 kb on + strand, within BWI76_RS03150 at 648.921 kb on - strand, within BWI76_RS03150 at 648.963 kb on + strand, within BWI76_RS03150 at 649.056 kb on - strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.112 kb on + strand, within BWI76_RS03150 at 649.151 kb on + strand, within BWI76_RS03150 at 649.159 kb on - strand, within BWI76_RS03150 at 649.206 kb on + strand, within BWI76_RS03150 at 649.214 kb on - strand, within BWI76_RS03150 at 649.225 kb on + strand, within BWI76_RS03150 at 649.225 kb on + strand, within BWI76_RS03150 at 649.233 kb on - strand, within BWI76_RS03150 at 649.233 kb on - strand, within BWI76_RS03150 at 649.248 kb on - strand, within BWI76_RS03150 at 649.277 kb on + strand, within BWI76_RS03150 at 649.287 kb on + strand, within BWI76_RS03150 at 649.295 kb on - strand, within BWI76_RS03150 at 649.329 kb on + strand, within BWI76_RS03150 at 649.363 kb on + strand, within BWI76_RS03150 at 649.371 kb on - strand, within BWI76_RS03150 at 649.489 kb on - strand, within BWI76_RS03150 at 649.500 kb on - strand, within BWI76_RS03150 at 649.500 kb on - strand, within BWI76_RS03150 at 649.519 kb on - strand, within BWI76_RS03150 at 649.523 kb on + strand, within BWI76_RS03150 at 649.577 kb on + strand, within BWI76_RS03150 at 649.585 kb on - strand, within BWI76_RS03150 at 649.585 kb on - strand, within BWI76_RS03150 at 649.619 kb on - strand, within BWI76_RS03150 at 649.691 kb on + strand, within BWI76_RS03150 at 649.704 kb on + strand, within BWI76_RS03150 at 649.706 kb on + strand, within BWI76_RS03150 at 649.706 kb on + strand, within BWI76_RS03150 at 649.711 kb on + strand, within BWI76_RS03150 at 649.714 kb on - strand, within BWI76_RS03150 at 649.714 kb on - strand, within BWI76_RS03150 at 649.723 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.746 kb on - strand, within BWI76_RS03150 at 649.748 kb on + strand, within BWI76_RS03150 at 649.751 kb on - strand, within BWI76_RS03150 at 649.751 kb on - strand, within BWI76_RS03150 at 649.751 kb on - strand at 649.783 kb on + strand, within BWI76_RS03150 at 649.783 kb on + strand, within BWI76_RS03150 at 649.798 kb on - strand, within BWI76_RS03150 at 649.853 kb on - strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.855 kb on - strand at 649.855 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.867 kb on - strand at 649.867 kb on - strand at 649.867 kb on - strand at 649.873 kb on + strand at 649.881 kb on - strand at 649.888 kb on + strand at 649.903 kb on - strand at 649.905 kb on + strand at 649.905 kb on + strand at 649.905 kb on + strand at 649.905 kb on - strand at 649.905 kb on - strand at 649.906 kb on - strand at 649.906 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.973 kb on + strand at 649.973 kb on + strand at 649.973 kb on + strand at 649.983 kb on + strand at 649.983 kb on + strand at 649.991 kb on - strand at 650.061 kb on - strand at 650.117 kb on - strand at 650.121 kb on + strand at 650.132 kb on + strand at 650.132 kb on + strand at 650.140 kb on - strand at 650.163 kb on + strand, within BWI76_RS03155 at 650.190 kb on + strand at 650.190 kb on + strand, within BWI76_RS03155 at 650.190 kb on + strand at 650.194 kb on + strand, within BWI76_RS03155 at 650.198 kb on + strand, within BWI76_RS03155 at 650.198 kb on + strand, within BWI76_RS03155 at 650.198 kb on - strand, within BWI76_RS03155 at 650.200 kb on + strand, within BWI76_RS03155 at 650.208 kb on - strand, within BWI76_RS03155 at 650.209 kb on - strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.221 kb on + strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.223 kb on - strand, within BWI76_RS03155 at 650.228 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.249 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.259 kb on + strand, within BWI76_RS03155 at 650.262 kb on - strand, within BWI76_RS03155 at 650.264 kb on - strand, within BWI76_RS03155 at 650.289 kb on - strand, within BWI76_RS03155 at 650.289 kb on - strand, within BWI76_RS03155 at 650.541 kb on + strand at 650.541 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.549 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.557 kb on + strand, within BWI76_RS03155 at 650.560 kb on - strand, within BWI76_RS03155 at 650.565 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 647,891 + BWI76_RS03140 0.70 -0.5 647,899 - BWI76_RS03140 0.71 -0.7 647,945 + BWI76_RS03140 0.76 +1.8 647,954 + BWI76_RS03140 0.77 -1.0 647,954 + BWI76_RS03140 0.77 -1.7 647,992 + BWI76_RS03140 0.80 -0.2 647,992 + BWI76_RS03140 0.80 -0.9 648,090 - BWI76_RS03140 0.90 +0.1 648,195 - -0.5 648,217 + -1.0 648,237 - +0.1 648,241 + +0.1 648,285 - +0.6 648,288 - -0.3 648,309 - +2.6 648,685 + +0.7 648,691 + +0.7 648,695 + -0.0 648,703 - +0.4 648,710 + -0.4 648,841 + BWI76_RS03150 0.13 +1.0 648,867 - BWI76_RS03150 0.15 -0.1 648,869 + BWI76_RS03150 0.15 +0.3 648,877 - BWI76_RS03150 0.16 +0.6 648,877 - BWI76_RS03150 0.16 +0.5 648,906 + BWI76_RS03150 0.18 +1.0 648,906 + BWI76_RS03150 0.18 +0.9 648,913 + BWI76_RS03150 0.19 -3.3 648,921 - BWI76_RS03150 0.19 -0.3 648,963 + BWI76_RS03150 0.23 -0.1 649,056 - BWI76_RS03150 0.30 -2.4 649,097 + BWI76_RS03150 0.33 -1.8 649,097 + BWI76_RS03150 0.33 -0.3 649,097 + BWI76_RS03150 0.33 -1.4 649,112 + BWI76_RS03150 0.35 -2.6 649,151 + BWI76_RS03150 0.38 -0.7 649,159 - BWI76_RS03150 0.38 +0.1 649,206 + BWI76_RS03150 0.42 +0.4 649,214 - BWI76_RS03150 0.43 +0.1 649,225 + BWI76_RS03150 0.44 +1.3 649,225 + BWI76_RS03150 0.44 -0.2 649,233 - BWI76_RS03150 0.44 +0.7 649,233 - BWI76_RS03150 0.44 +1.0 649,248 - BWI76_RS03150 0.45 -0.0 649,277 + BWI76_RS03150 0.48 -1.2 649,287 + BWI76_RS03150 0.48 +0.2 649,295 - BWI76_RS03150 0.49 -0.0 649,329 + BWI76_RS03150 0.52 -0.4 649,363 + BWI76_RS03150 0.54 -1.1 649,371 - BWI76_RS03150 0.55 +0.5 649,489 - BWI76_RS03150 0.64 -0.5 649,500 - BWI76_RS03150 0.65 +0.7 649,500 - BWI76_RS03150 0.65 -0.4 649,519 - BWI76_RS03150 0.67 +2.3 649,523 + BWI76_RS03150 0.67 +1.2 649,577 + BWI76_RS03150 0.71 -0.3 649,585 - BWI76_RS03150 0.72 +1.0 649,585 - BWI76_RS03150 0.72 +0.1 649,619 - BWI76_RS03150 0.75 -1.5 649,691 + BWI76_RS03150 0.80 +0.0 649,704 + BWI76_RS03150 0.81 +0.2 649,706 + BWI76_RS03150 0.82 -0.7 649,706 + BWI76_RS03150 0.82 -0.0 649,711 + BWI76_RS03150 0.82 +0.1 649,714 - BWI76_RS03150 0.82 -1.1 649,714 - BWI76_RS03150 0.82 -0.1 649,723 + BWI76_RS03150 0.83 -0.7 649,743 + BWI76_RS03150 0.85 -0.6 649,743 + BWI76_RS03150 0.85 -1.4 649,743 + BWI76_RS03150 0.85 +0.3 649,746 - BWI76_RS03150 0.85 -0.8 649,748 + BWI76_RS03150 0.85 +2.2 649,751 - BWI76_RS03150 0.85 -1.5 649,751 - BWI76_RS03150 0.85 -0.9 649,751 - +1.4 649,783 + BWI76_RS03150 0.88 -0.7 649,783 + BWI76_RS03150 0.88 -0.1 649,798 - BWI76_RS03150 0.89 +0.6 649,853 - +0.6 649,854 + +1.5 649,854 + +0.5 649,854 + -0.1 649,854 + +0.5 649,854 + -4.0 649,854 + -0.8 649,854 + -1.3 649,854 + +0.0 649,854 + -1.6 649,854 + -0.9 649,854 + -0.0 649,854 + -0.9 649,854 + +2.0 649,855 - -1.6 649,855 - -0.5 649,862 - +0.4 649,862 - -0.0 649,862 - +0.8 649,862 - +1.1 649,862 - +0.2 649,862 - +1.0 649,862 - +1.4 649,862 - -0.9 649,862 - -0.0 649,862 - +1.2 649,862 - -0.2 649,867 - -1.2 649,867 - +0.5 649,867 - +0.3 649,873 + -0.6 649,881 - +1.0 649,888 + +2.0 649,903 - +2.4 649,905 + -1.9 649,905 + -0.6 649,905 + +1.1 649,905 - -0.3 649,905 - +0.1 649,906 - +0.7 649,906 - +0.7 649,913 - -2.2 649,913 - -0.1 649,913 - +1.4 649,913 - -0.1 649,913 - +1.3 649,973 + -0.5 649,973 + -0.3 649,973 + +2.5 649,983 + -0.4 649,983 + +0.1 649,991 - -1.6 650,061 - -0.8 650,117 - +1.0 650,121 + -0.4 650,132 + -0.7 650,132 + -0.6 650,140 - -0.1 650,163 + BWI76_RS03155 0.11 -0.0 650,190 + +1.4 650,190 + BWI76_RS03155 0.14 -0.8 650,190 + +0.4 650,194 + BWI76_RS03155 0.14 +0.0 650,198 + BWI76_RS03155 0.15 -0.5 650,198 + BWI76_RS03155 0.15 -1.2 650,198 - BWI76_RS03155 0.15 +0.2 650,200 + BWI76_RS03155 0.15 +0.0 650,208 - BWI76_RS03155 0.16 +0.4 650,209 - BWI76_RS03155 0.16 +0.4 650,213 + BWI76_RS03155 0.17 -0.2 650,213 + BWI76_RS03155 0.17 +0.5 650,213 + BWI76_RS03155 0.17 -0.9 650,215 + BWI76_RS03155 0.17 -0.6 650,215 + BWI76_RS03155 0.17 +0.4 650,215 + BWI76_RS03155 0.17 -0.2 650,221 + BWI76_RS03155 0.17 -0.1 650,221 - BWI76_RS03155 0.17 +0.2 650,221 - BWI76_RS03155 0.17 -0.2 650,221 - BWI76_RS03155 0.17 -0.4 650,221 - BWI76_RS03155 0.17 +0.1 650,223 - BWI76_RS03155 0.18 -1.4 650,228 + BWI76_RS03155 0.18 -0.8 650,236 + BWI76_RS03155 0.19 -2.0 650,236 + BWI76_RS03155 0.19 -0.2 650,236 + BWI76_RS03155 0.19 -1.2 650,236 + BWI76_RS03155 0.19 -0.7 650,236 + BWI76_RS03155 0.19 +0.5 650,244 - BWI76_RS03155 0.20 -2.0 650,244 - BWI76_RS03155 0.20 +1.6 650,244 - BWI76_RS03155 0.20 -1.0 650,244 - BWI76_RS03155 0.20 -0.1 650,244 - BWI76_RS03155 0.20 -0.8 650,246 + BWI76_RS03155 0.20 +0.2 650,246 + -0.1 650,246 + BWI76_RS03155 0.20 -1.2 650,246 + BWI76_RS03155 0.20 -0.4 650,246 + BWI76_RS03155 0.20 -0.1 650,246 + BWI76_RS03155 0.20 -0.4 650,246 + BWI76_RS03155 0.20 +2.2 650,246 + BWI76_RS03155 0.20 +1.2 650,249 - BWI76_RS03155 0.20 -0.0 650,254 - BWI76_RS03155 0.21 +0.4 650,254 - BWI76_RS03155 0.21 +2.5 650,254 - BWI76_RS03155 0.21 +0.2 650,254 - BWI76_RS03155 0.21 -0.9 650,254 - BWI76_RS03155 0.21 -0.6 650,254 - BWI76_RS03155 0.21 +0.4 650,254 - BWI76_RS03155 0.21 -1.5 650,254 - BWI76_RS03155 0.21 -0.1 650,254 - BWI76_RS03155 0.21 -2.0 650,259 + BWI76_RS03155 0.22 -1.8 650,262 - BWI76_RS03155 0.22 +2.7 650,264 - BWI76_RS03155 0.22 +0.2 650,289 - BWI76_RS03155 0.25 -0.7 650,289 - BWI76_RS03155 0.25 +1.7 650,541 + +0.8 650,541 + BWI76_RS03155 0.53 -0.6 650,542 + BWI76_RS03155 0.53 -1.4 650,542 + BWI76_RS03155 0.53 -0.4 650,549 - BWI76_RS03155 0.54 +2.7 650,550 - BWI76_RS03155 0.54 +0.6 650,550 - BWI76_RS03155 0.54 +2.0 650,557 + BWI76_RS03155 0.55 -1.8 650,560 - BWI76_RS03155 0.55 +0.8 650,565 - BWI76_RS03155 0.55 -0.4 650,807 - BWI76_RS03155 0.82 +1.4 650,807 - BWI76_RS03155 0.82 +0.4 650,877 + -0.6
Or see this region's nucleotide sequence