Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotides BWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotides
BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563
_RS01090
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
Position (kb) 225
226
227 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 224.637 kb on - strand, within BWI76_RS01090 at 224.826 kb on - strand, within BWI76_RS01090 at 224.859 kb on + strand, within BWI76_RS01090 at 225.000 kb on - strand, within BWI76_RS01090 at 225.010 kb on + strand, within BWI76_RS01090 at 225.020 kb on + strand, within BWI76_RS01090 at 225.502 kb on + strand at 225.589 kb on + strand at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095 at 226.711 kb on + strand at 226.776 kb on + strand at 226.901 kb on + strand at 226.918 kb on + strand at 226.979 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.140 kb on - strand, within BWI76_RS01100 at 227.142 kb on + strand, within BWI76_RS01100 at 227.227 kb on + strand, within BWI76_RS01100 at 227.235 kb on - strand, within BWI76_RS01100 at 227.642 kb on - strand, within BWI76_RS01100
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 224,637 - BWI76_RS01090 0.18 -2.5 224,826 - BWI76_RS01090 0.35 -0.3 224,859 + BWI76_RS01090 0.38 -0.6 225,000 - BWI76_RS01090 0.50 -0.8 225,010 + BWI76_RS01090 0.51 +0.2 225,020 + BWI76_RS01090 0.52 +2.3 225,502 + -0.1 225,589 + -0.4 226,034 - BWI76_RS01095 0.38 +0.5 226,533 + BWI76_RS01095 0.77 -1.7 226,541 - BWI76_RS01095 0.78 -1.5 226,711 + -0.3 226,776 + -0.3 226,901 + -0.3 226,918 + -1.0 226,979 + BWI76_RS01100 0.15 -3.8 227,132 + BWI76_RS01100 0.29 -1.0 227,132 + BWI76_RS01100 0.29 +1.3 227,132 + BWI76_RS01100 0.29 +0.5 227,140 - BWI76_RS01100 0.30 +1.1 227,142 + BWI76_RS01100 0.30 +0.6 227,227 + BWI76_RS01100 0.38 +0.4 227,235 - BWI76_RS01100 0.39 +1.0 227,642 - BWI76_RS01100 0.77 -0.7
Or see this region's nucleotide sequence