Experiment: B-glucan from yeast; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1844 and BT1845 are separated by 160 nucleotides BT1845 and BT1846 are separated by 8 nucleotides BT1846 and BT1847 are separated by 115 nucleotides BT1847 and BT1848 are separated by 211 nucleotides
BT1844: BT1844 - transcriptional regulator, putative iron uptake regulation protein (NCBI ptt file), at 2,316,050 to 2,316,541
BT1844
BT1845: BT1845 - conserved hypothetical protein (NCBI ptt file), at 2,316,702 to 2,317,367
BT1845
BT1846: BT1846 - putative dipeptidyl-peptidase III (NCBI ptt file), at 2,317,376 to 2,319,403
BT1846
BT1847: BT1847 - transcriptional regulator, AraC/XylS family (NCBI ptt file), at 2,319,519 to 2,319,983
BT1847
BT1848: BT1848 - ATP-dependent DNA helicase recQ (NCBI ptt file), at 2,320,195 to 2,322,057
BT1848
Position (kb)
2317
2318
2319
2320 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2316.394 kb on - strand, within BT1844 at 2316.400 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.490 kb on - strand, within BT1844 at 2316.514 kb on + strand at 2316.568 kb on + strand at 2316.607 kb on + strand at 2316.612 kb on - strand at 2316.618 kb on + strand at 2316.619 kb on - strand at 2316.625 kb on + strand at 2316.628 kb on + strand at 2316.632 kb on + strand at 2316.633 kb on - strand at 2316.642 kb on - strand at 2316.642 kb on - strand at 2316.645 kb on + strand at 2316.682 kb on + strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.687 kb on - strand at 2316.693 kb on + strand at 2316.697 kb on - strand at 2316.697 kb on - strand at 2316.700 kb on + strand at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.826 kb on + strand, within BT1845 at 2316.827 kb on - strand, within BT1845 at 2317.103 kb on + strand, within BT1845 at 2317.360 kb on - strand at 2317.382 kb on - strand at 2317.405 kb on - strand at 2317.437 kb on + strand at 2317.447 kb on + strand at 2317.501 kb on - strand at 2317.525 kb on - strand at 2317.549 kb on - strand at 2317.882 kb on - strand, within BT1846 at 2317.892 kb on - strand, within BT1846 at 2317.892 kb on - strand, within BT1846 at 2317.893 kb on + strand, within BT1846 at 2318.032 kb on - strand, within BT1846 at 2318.170 kb on + strand, within BT1846 at 2318.174 kb on - strand, within BT1846 at 2318.175 kb on + strand, within BT1846 at 2318.176 kb on - strand, within BT1846 at 2318.201 kb on - strand, within BT1846 at 2318.201 kb on - strand, within BT1846 at 2318.237 kb on - strand, within BT1846 at 2318.319 kb on - strand, within BT1846 at 2318.327 kb on + strand, within BT1846 at 2318.328 kb on - strand, within BT1846 at 2318.545 kb on - strand, within BT1846 at 2318.545 kb on - strand, within BT1846 at 2318.691 kb on - strand, within BT1846 at 2318.691 kb on - strand, within BT1846 at 2318.794 kb on - strand, within BT1846 at 2318.794 kb on - strand, within BT1846 at 2318.880 kb on - strand, within BT1846 at 2318.880 kb on - strand, within BT1846 at 2319.059 kb on - strand, within BT1846 at 2319.087 kb on + strand, within BT1846 at 2319.142 kb on - strand, within BT1846 at 2319.142 kb on - strand, within BT1846 at 2319.176 kb on - strand, within BT1846 at 2319.302 kb on + strand at 2319.389 kb on - strand at 2319.429 kb on - strand at 2319.835 kb on - strand, within BT1847 at 2320.019 kb on - strand at 2320.172 kb on - strand at 2320.221 kb on + strand at 2320.302 kb on - strand at 2320.350 kb on - strand at 2320.372 kb on - strand at 2320.384 kb on + strand, within BT1848
Per-strain Table
Position Strand Gene LocusTag Fraction B-glucan from yeast; autoclaved 2.5 mg/ml (C) remove 2,316,394 - BT1844 0.70 -1.6 2,316,400 + BT1844 0.71 -1.6 2,316,429 + BT1844 0.77 -2.1 2,316,429 + BT1844 0.77 -3.6 2,316,490 - BT1844 0.89 +0.0 2,316,514 + +0.0 2,316,568 + -1.4 2,316,607 + -0.3 2,316,612 - -3.4 2,316,618 + -0.8 2,316,619 - -3.0 2,316,625 + -1.3 2,316,628 + +1.0 2,316,632 + -0.0 2,316,633 - -1.0 2,316,642 - +0.0 2,316,642 - -3.4 2,316,645 + -1.6 2,316,682 + -1.2 2,316,683 - -1.8 2,316,683 - -2.6 2,316,683 - -1.0 2,316,687 - -4.4 2,316,693 + -2.7 2,316,697 - -1.6 2,316,697 - -1.3 2,316,700 + -2.0 2,316,825 - BT1845 0.18 -1.6 2,316,825 - BT1845 0.18 +1.6 2,316,825 - BT1845 0.18 -0.6 2,316,826 + BT1845 0.19 -2.6 2,316,827 - BT1845 0.19 +1.0 2,317,103 + BT1845 0.60 -3.0 2,317,360 - +0.0 2,317,382 - -0.7 2,317,405 - -1.0 2,317,437 + +0.0 2,317,447 + -1.0 2,317,501 - +0.0 2,317,525 - +0.0 2,317,549 - -1.0 2,317,882 - BT1846 0.25 -0.6 2,317,892 - BT1846 0.25 +0.6 2,317,892 - BT1846 0.25 +0.0 2,317,893 + BT1846 0.25 +1.0 2,318,032 - BT1846 0.32 +0.5 2,318,170 + BT1846 0.39 +0.0 2,318,174 - BT1846 0.39 -3.9 2,318,175 + BT1846 0.39 +0.0 2,318,176 - BT1846 0.39 +0.2 2,318,201 - BT1846 0.41 +2.0 2,318,201 - BT1846 0.41 -0.6 2,318,237 - BT1846 0.42 -1.9 2,318,319 - BT1846 0.46 -1.6 2,318,327 + BT1846 0.47 +1.0 2,318,328 - BT1846 0.47 -1.6 2,318,545 - BT1846 0.58 +0.2 2,318,545 - BT1846 0.58 -1.6 2,318,691 - BT1846 0.65 +0.0 2,318,691 - BT1846 0.65 -1.0 2,318,794 - BT1846 0.70 -3.0 2,318,794 - BT1846 0.70 +0.0 2,318,880 - BT1846 0.74 +0.0 2,318,880 - BT1846 0.74 +0.9 2,319,059 - BT1846 0.83 -1.6 2,319,087 + BT1846 0.84 +2.6 2,319,142 - BT1846 0.87 +0.0 2,319,142 - BT1846 0.87 -1.4 2,319,176 - BT1846 0.89 +0.8 2,319,302 + -0.4 2,319,389 - +0.0 2,319,429 - -1.0 2,319,835 - BT1847 0.68 +0.0 2,320,019 - +0.6 2,320,172 - -0.4 2,320,221 + +0.0 2,320,302 - +0.1 2,320,350 - +0.0 2,320,372 - -2.6 2,320,384 + BT1848 0.10 +0.0
Or see this region's nucleotide sequence