Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35330 and BPHYT_RS35335 are separated by 89 nucleotides BPHYT_RS35335 and BPHYT_RS35340 are separated by 40 nucleotides BPHYT_RS35340 and BPHYT_RS35345 overlap by 4 nucleotides
BPHYT_RS35330: BPHYT_RS35330 - purine permease, at 3,525,282 to 3,526,622
_RS35330
BPHYT_RS35335: BPHYT_RS35335 - cobalamin biosynthesis protein, at 3,526,712 to 3,527,794
_RS35335
BPHYT_RS35340: BPHYT_RS35340 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,527,835 to 3,528,425
_RS35340
BPHYT_RS35345: BPHYT_RS35345 - decarboxylase UbiD, at 3,528,422 to 3,529,816
_RS35345
Position (kb) 3526
3527
3528 Strain fitness (log2 ratio) -2
-1
0
1 at 3526.639 kb on - strand at 3527.187 kb on + strand, within BPHYT_RS35335 at 3527.187 kb on + strand, within BPHYT_RS35335 at 3527.291 kb on - strand, within BPHYT_RS35335 at 3527.697 kb on + strand at 3527.833 kb on + strand at 3527.845 kb on + strand at 3527.846 kb on - strand at 3528.015 kb on + strand, within BPHYT_RS35340 at 3528.066 kb on + strand, within BPHYT_RS35340 at 3528.236 kb on - strand, within BPHYT_RS35340 at 3528.361 kb on - strand, within BPHYT_RS35340 at 3528.604 kb on + strand, within BPHYT_RS35345
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 3,526,639 - -2.3 3,527,187 + BPHYT_RS35335 0.44 +0.1 3,527,187 + BPHYT_RS35335 0.44 -1.0 3,527,291 - BPHYT_RS35335 0.53 +0.7 3,527,697 + +0.5 3,527,833 + -0.4 3,527,845 + +0.2 3,527,846 - +1.4 3,528,015 + BPHYT_RS35340 0.30 -0.4 3,528,066 + BPHYT_RS35340 0.39 +0.0 3,528,236 - BPHYT_RS35340 0.68 -0.2 3,528,361 - BPHYT_RS35340 0.89 -1.5 3,528,604 + BPHYT_RS35345 0.13 -0.1
Or see this region's nucleotide sequence