Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01770 and BPHYT_RS01775 are separated by 378 nucleotides BPHYT_RS01775 and BPHYT_RS01780 overlap by 4 nucleotides BPHYT_RS01780 and BPHYT_RS01785 are separated by 375 nucleotides
BPHYT_RS01770: BPHYT_RS01770 - carbon monoxide dehydrogenase, at 403,572 to 404,090
_RS01770
BPHYT_RS01775: BPHYT_RS01775 - hypothetical protein, at 404,469 to 404,813
_RS01775
BPHYT_RS01780: BPHYT_RS01780 - hypothetical protein, at 404,810 to 405,178
_RS01780
BPHYT_RS01785: BPHYT_RS01785 - amino acid permease, at 405,554 to 406,945
_RS01785
Position (kb) 404
405
406 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 404.202 kb on - strand at 404.226 kb on + strand at 404.305 kb on - strand at 404.307 kb on + strand at 404.307 kb on + strand at 404.308 kb on - strand at 404.335 kb on - strand at 404.406 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.426 kb on - strand at 404.470 kb on + strand at 404.588 kb on + strand, within BPHYT_RS01775 at 404.589 kb on - strand, within BPHYT_RS01775 at 404.596 kb on + strand, within BPHYT_RS01775 at 404.597 kb on - strand, within BPHYT_RS01775 at 404.705 kb on - strand, within BPHYT_RS01775 at 404.717 kb on + strand, within BPHYT_RS01775 at 404.718 kb on - strand, within BPHYT_RS01775 at 404.720 kb on + strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.820 kb on - strand at 404.867 kb on + strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.876 kb on - strand, within BPHYT_RS01780 at 405.024 kb on - strand, within BPHYT_RS01780 at 405.144 kb on - strand at 405.146 kb on - strand at 405.214 kb on + strand at 405.214 kb on + strand at 405.284 kb on - strand at 405.325 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.527 kb on + strand at 405.797 kb on - strand, within BPHYT_RS01785 at 405.880 kb on + strand at 405.986 kb on + strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 406.069 kb on - strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 404,202 - +0.1 404,226 + +0.2 404,305 - -0.1 404,307 + -0.5 404,307 + -0.2 404,308 - +0.2 404,335 - +0.4 404,406 + -0.2 404,425 + -0.2 404,425 + -0.6 404,425 + -0.4 404,425 + -0.6 404,426 - -0.3 404,470 + +0.1 404,588 + BPHYT_RS01775 0.34 -0.1 404,589 - BPHYT_RS01775 0.35 +0.2 404,596 + BPHYT_RS01775 0.37 +0.0 404,597 - BPHYT_RS01775 0.37 -0.2 404,705 - BPHYT_RS01775 0.68 -0.1 404,717 + BPHYT_RS01775 0.72 +0.7 404,718 - BPHYT_RS01775 0.72 -0.7 404,720 + BPHYT_RS01775 0.73 +0.6 404,721 - BPHYT_RS01775 0.73 -2.1 404,721 - BPHYT_RS01775 0.73 -0.5 404,820 - -1.8 404,867 + BPHYT_RS01780 0.15 -0.4 404,868 - BPHYT_RS01780 0.16 +8.5 404,868 - BPHYT_RS01780 0.16 -0.6 404,868 - BPHYT_RS01780 0.16 -0.5 404,876 - BPHYT_RS01780 0.18 +0.1 405,024 - BPHYT_RS01780 0.58 -0.4 405,144 - -0.6 405,146 - -1.0 405,214 + +0.7 405,214 + +0.1 405,284 - +0.1 405,325 - +0.4 405,373 + +0.1 405,373 + -0.2 405,527 + -0.5 405,797 - BPHYT_RS01785 0.17 -0.0 405,880 + +0.6 405,986 + BPHYT_RS01785 0.31 +0.2 405,987 - BPHYT_RS01785 0.31 -0.4 405,987 - BPHYT_RS01785 0.31 -0.2 405,987 - BPHYT_RS01785 0.31 -0.4 406,069 - BPHYT_RS01785 0.37 -0.1 406,143 + BPHYT_RS01785 0.42 +0.4 406,143 + BPHYT_RS01785 0.42 -0.2 406,143 + BPHYT_RS01785 0.42 -0.3 406,143 + BPHYT_RS01785 0.42 -0.9 406,143 + BPHYT_RS01785 0.42 -0.9 406,144 - BPHYT_RS01785 0.42 -0.9 406,144 - BPHYT_RS01785 0.42 +0.1
Or see this region's nucleotide sequence