Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13675

Experiment: Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13665 and BPHYT_RS13670 are separated by 287 nucleotidesBPHYT_RS13670 and BPHYT_RS13675 are separated by 2 nucleotidesBPHYT_RS13675 and BPHYT_RS13680 are separated by 243 nucleotidesBPHYT_RS13680 and BPHYT_RS13685 are separated by 142 nucleotides BPHYT_RS13665: BPHYT_RS13665 - alpha/beta hydrolase, at 3,114,527 to 3,115,441 _RS13665 BPHYT_RS13670: BPHYT_RS13670 - succinyl-CoA:3-ketoacid-CoA transferase, at 3,115,729 to 3,116,370 _RS13670 BPHYT_RS13675: BPHYT_RS13675 - succinyl-CoA:3-ketoacid-CoA transferase, at 3,116,373 to 3,117,077 _RS13675 BPHYT_RS13680: BPHYT_RS13680 - LuxR family transcriptional regulator, at 3,117,321 to 3,117,866 _RS13680 BPHYT_RS13685: BPHYT_RS13685 - thioesterase, at 3,118,009 to 3,118,440 _RS13685 Position (kb) 3116 3117 3118Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3115.386 kb on + strandat 3115.520 kb on + strandat 3115.520 kb on + strandat 3115.521 kb on - strandat 3115.521 kb on - strandat 3116.463 kb on - strand, within BPHYT_RS13675at 3116.653 kb on + strand, within BPHYT_RS13675at 3116.653 kb on + strandat 3116.654 kb on - strand, within BPHYT_RS13675at 3116.685 kb on - strand, within BPHYT_RS13675at 3116.685 kb on - strand, within BPHYT_RS13675at 3116.933 kb on + strand, within BPHYT_RS13675at 3116.934 kb on - strand, within BPHYT_RS13675at 3117.064 kb on + strandat 3117.081 kb on + strandat 3117.081 kb on + strandat 3117.081 kb on + strandat 3117.081 kb on + strandat 3117.081 kb on + strandat 3117.081 kb on + strandat 3117.082 kb on - strandat 3117.082 kb on - strandat 3117.082 kb on - strandat 3117.128 kb on + strandat 3117.129 kb on - strandat 3117.165 kb on + strandat 3117.165 kb on + strandat 3117.166 kb on - strandat 3117.166 kb on - strandat 3117.166 kb on - strandat 3117.166 kb on - strandat 3117.262 kb on - strandat 3117.262 kb on - strandat 3117.297 kb on + strandat 3117.301 kb on + strandat 3117.301 kb on + strandat 3117.301 kb on + strandat 3117.301 kb on + strandat 3117.302 kb on - strandat 3117.305 kb on + strandat 3117.306 kb on - strandat 3117.404 kb on + strand, within BPHYT_RS13680at 3117.404 kb on + strand, within BPHYT_RS13680at 3117.404 kb on + strand, within BPHYT_RS13680at 3117.474 kb on + strand, within BPHYT_RS13680at 3117.474 kb on + strand, within BPHYT_RS13680at 3117.475 kb on - strand, within BPHYT_RS13680at 3117.544 kb on - strand, within BPHYT_RS13680at 3117.793 kb on - strand, within BPHYT_RS13680at 3117.816 kb on + strandat 3117.816 kb on + strandat 3118.007 kb on + strandat 3118.008 kb on - strandat 3118.010 kb on + strandat 3118.010 kb on + strandat 3118.010 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
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3,115,386 + +1.1
3,115,520 + -0.5
3,115,520 + +1.0
3,115,521 - -0.1
3,115,521 - +0.1
3,116,463 - BPHYT_RS13675 0.13 -0.6
3,116,653 + BPHYT_RS13675 0.40 -0.7
3,116,653 + +0.5
3,116,654 - BPHYT_RS13675 0.40 -0.5
3,116,685 - BPHYT_RS13675 0.44 +0.5
3,116,685 - BPHYT_RS13675 0.44 -0.7
3,116,933 + BPHYT_RS13675 0.79 -1.3
3,116,934 - BPHYT_RS13675 0.80 -1.3
3,117,064 + +2.1
3,117,081 + -3.2
3,117,081 + -0.2
3,117,081 + +0.4
3,117,081 + -1.1
3,117,081 + -1.6
3,117,081 + -2.3
3,117,082 - +0.3
3,117,082 - +1.8
3,117,082 - -0.1
3,117,128 + -2.1
3,117,129 - -1.0
3,117,165 + +0.3
3,117,165 + -0.1
3,117,166 - +0.3
3,117,166 - -0.1
3,117,166 - +0.4
3,117,166 - -0.1
3,117,262 - -1.5
3,117,262 - +0.3
3,117,297 + -0.3
3,117,301 + -0.1
3,117,301 + +0.5
3,117,301 + -1.0
3,117,301 + -0.5
3,117,302 - +0.5
3,117,305 + +0.7
3,117,306 - +1.1
3,117,404 + BPHYT_RS13680 0.15 +0.2
3,117,404 + BPHYT_RS13680 0.15 +0.7
3,117,404 + BPHYT_RS13680 0.15 -2.1
3,117,474 + BPHYT_RS13680 0.28 +0.4
3,117,474 + BPHYT_RS13680 0.28 -0.8
3,117,475 - BPHYT_RS13680 0.28 +0.4
3,117,544 - BPHYT_RS13680 0.41 +0.3
3,117,793 - BPHYT_RS13680 0.86 -0.5
3,117,816 + +0.8
3,117,816 + +0.5
3,118,007 + -0.7
3,118,008 - +0.8
3,118,010 + -1.1
3,118,010 + +0.3
3,118,010 + +0.8

Or see this region's nucleotide sequence