Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotides BPHYT_RS25505 and BPHYT_RS25510 are separated by 16 nucleotides BPHYT_RS25510 and BPHYT_RS25515 are separated by 83 nucleotides BPHYT_RS25515 and BPHYT_RS25520 are separated by 172 nucleotides
BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807
_RS25500
BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349
_RS25505
BPHYT_RS25510: BPHYT_RS25510 - hypothetical protein, at 1,298,366 to 1,298,686
_RS25510
BPHYT_RS25515: BPHYT_RS25515 - glutathione S-transferase, at 1,298,770 to 1,299,471
_RS25515
BPHYT_RS25520: BPHYT_RS25520 - TetR family transcriptional regulator, at 1,299,644 to 1,300,237
_RS25520
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-1
0
1 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1299.243 kb on - strand, within BPHYT_RS25515 at 1299.710 kb on + strand, within BPHYT_RS25520 at 1299.717 kb on - strand, within BPHYT_RS25520 at 1299.718 kb on + strand, within BPHYT_RS25520 at 1299.724 kb on + strand, within BPHYT_RS25520 at 1300.370 kb on + strand at 1300.371 kb on - strand at 1300.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 1,298,100 + BPHYT_RS25505 0.54 +0.1 1,298,100 + BPHYT_RS25505 0.54 -0.3 1,299,243 - BPHYT_RS25515 0.67 -0.7 1,299,710 + BPHYT_RS25520 0.11 +0.7 1,299,717 - BPHYT_RS25520 0.12 -0.8 1,299,718 + BPHYT_RS25520 0.12 -0.2 1,299,724 + BPHYT_RS25520 0.13 -0.2 1,300,370 + -0.5 1,300,371 - +0.5 1,300,460 - +1.1
Or see this region's nucleotide sequence