Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25285 and BPHYT_RS25290 are separated by 115 nucleotides BPHYT_RS25290 and BPHYT_RS25295 are separated by 14 nucleotides BPHYT_RS25295 and BPHYT_RS25300 are separated by 57 nucleotides BPHYT_RS25300 and BPHYT_RS25305 are separated by 12 nucleotides
BPHYT_RS25285: BPHYT_RS25285 - AraC family transcriptional regulator, at 1,249,609 to 1,250,643
_RS25285
BPHYT_RS25290: BPHYT_RS25290 - membrane protein, at 1,250,759 to 1,251,451
_RS25290
BPHYT_RS25295: BPHYT_RS25295 - cobalt transporter, at 1,251,466 to 1,251,675
_RS25295
BPHYT_RS25300: BPHYT_RS25300 - transmembrane nitrile hydratase, at 1,251,733 to 1,252,113
_RS25300
BPHYT_RS25305: BPHYT_RS25305 - cobalt-containing nitrile hydratase subunit alpha, at 1,252,126 to 1,252,749
_RS25305
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-2
-1
0
1 at 1249.973 kb on + strand, within BPHYT_RS25285 at 1249.973 kb on + strand, within BPHYT_RS25285 at 1250.529 kb on - strand, within BPHYT_RS25285 at 1250.552 kb on - strand at 1250.686 kb on - strand at 1251.344 kb on + strand, within BPHYT_RS25290 at 1251.345 kb on - strand, within BPHYT_RS25290 at 1251.345 kb on - strand, within BPHYT_RS25290 at 1251.437 kb on + strand at 1251.521 kb on + strand, within BPHYT_RS25295 at 1252.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 1,249,973 + BPHYT_RS25285 0.35 -0.3 1,249,973 + BPHYT_RS25285 0.35 +0.8 1,250,529 - BPHYT_RS25285 0.89 -0.1 1,250,552 - -0.3 1,250,686 - -0.2 1,251,344 + BPHYT_RS25290 0.84 -0.6 1,251,345 - BPHYT_RS25290 0.85 +1.4 1,251,345 - BPHYT_RS25290 0.85 -2.0 1,251,437 + -0.0 1,251,521 + BPHYT_RS25295 0.26 -0.5 1,252,092 - +0.7
Or see this region's nucleotide sequence