Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21640 and BPHYT_RS21645 are separated by 119 nucleotides BPHYT_RS21645 and BPHYT_RS21650 are separated by 31 nucleotides BPHYT_RS21650 and BPHYT_RS21655 overlap by 4 nucleotides
BPHYT_RS21640: BPHYT_RS21640 - alpha/beta hydrolase, at 409,847 to 410,698
_RS21640
BPHYT_RS21645: BPHYT_RS21645 - LysR family transcriptional regulator, at 410,818 to 411,645
_RS21645
BPHYT_RS21650: BPHYT_RS21650 - cobalamin synthesis protein P47K, at 411,677 to 412,609
_RS21650
BPHYT_RS21655: BPHYT_RS21655 - amidohydrolase, at 412,606 to 414,264
_RS21655
Position (kb) 410
411
412 Strain fitness (log2 ratio) -1
0
1 at 410.554 kb on - strand, within BPHYT_RS21640 at 410.617 kb on - strand at 410.741 kb on + strand at 410.756 kb on + strand at 411.001 kb on + strand, within BPHYT_RS21645 at 411.098 kb on - strand, within BPHYT_RS21645 at 411.146 kb on - strand, within BPHYT_RS21645 at 411.225 kb on - strand, within BPHYT_RS21645 at 411.314 kb on + strand, within BPHYT_RS21645 at 411.314 kb on + strand, within BPHYT_RS21645 at 411.927 kb on + strand, within BPHYT_RS21650 at 411.927 kb on + strand, within BPHYT_RS21650 at 412.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 410,554 - BPHYT_RS21640 0.83 +0.6 410,617 - +0.6 410,741 + +0.7 410,756 + +0.8 411,001 + BPHYT_RS21645 0.22 -0.2 411,098 - BPHYT_RS21645 0.34 -1.4 411,146 - BPHYT_RS21645 0.40 -1.0 411,225 - BPHYT_RS21645 0.49 +1.3 411,314 + BPHYT_RS21645 0.60 -1.4 411,314 + BPHYT_RS21645 0.60 +0.5 411,927 + BPHYT_RS21650 0.27 +0.6 411,927 + BPHYT_RS21650 0.27 -0.8 412,540 + +0.2
Or see this region's nucleotide sequence