Experiment: LB 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09615 and BPHYT_RS09620 are separated by 78 nucleotides BPHYT_RS09620 and BPHYT_RS09625 overlap by 1 nucleotides BPHYT_RS09625 and BPHYT_RS09630 are separated by 94 nucleotides
BPHYT_RS09615: BPHYT_RS09615 - folate-binding protein, at 2,180,802 to 2,181,875
_RS09615
BPHYT_RS09620: BPHYT_RS09620 - aminodeoxychorismate lyase, at 2,181,954 to 2,182,964
_RS09620
BPHYT_RS09625: BPHYT_RS09625 - thymidylate kinase, at 2,182,964 to 2,183,584
_RS09625
BPHYT_RS09630: BPHYT_RS09630 - DNA polymerase III subunit delta', at 2,183,679 to 2,184,713
_RS09630
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-2
-1
0
1 at 2181.926 kb on + strand at 2182.451 kb on + strand, within BPHYT_RS09620 at 2182.725 kb on + strand, within BPHYT_RS09620 at 2182.725 kb on + strand, within BPHYT_RS09620 at 2182.771 kb on - strand, within BPHYT_RS09620 at 2182.775 kb on + strand, within BPHYT_RS09620 at 2182.779 kb on + strand, within BPHYT_RS09620 at 2182.947 kb on + strand at 2183.609 kb on + strand at 2183.609 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 37C remove 2,181,926 + -0.8 2,182,451 + BPHYT_RS09620 0.49 +0.8 2,182,725 + BPHYT_RS09620 0.76 -0.5 2,182,725 + BPHYT_RS09620 0.76 -1.3 2,182,771 - BPHYT_RS09620 0.81 -2.6 2,182,775 + BPHYT_RS09620 0.81 -1.6 2,182,779 + BPHYT_RS09620 0.82 +1.0 2,182,947 + -1.0 2,183,609 + +0.8 2,183,609 + +0.8
Or see this region's nucleotide sequence