Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12350

Experiment: Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12325 and BPHYT_RS12330 are separated by 142 nucleotidesBPHYT_RS12330 and BPHYT_RS12335 are separated by 94 nucleotidesBPHYT_RS12335 and BPHYT_RS12340 are separated by 61 nucleotidesBPHYT_RS12340 and BPHYT_RS12345 are separated by 97 nucleotidesBPHYT_RS12345 and BPHYT_RS12350 are separated by 155 nucleotidesBPHYT_RS12350 and BPHYT_RS12355 are separated by 122 nucleotides BPHYT_RS12325: BPHYT_RS12325 - GNAT family acetyltransferase, at 2,814,299 to 2,814,763 _RS12325 BPHYT_RS12330: BPHYT_RS12330 - tRNA-Asp, at 2,814,906 to 2,814,982 _RS12330 BPHYT_RS12335: BPHYT_RS12335 - tRNA-Glu, at 2,815,077 to 2,815,152 _RS12335 BPHYT_RS12340: BPHYT_RS12340 - tRNA-Asp, at 2,815,214 to 2,815,290 _RS12340 BPHYT_RS12345: BPHYT_RS12345 - tRNA-Glu, at 2,815,388 to 2,815,463 _RS12345 BPHYT_RS12350: BPHYT_RS12350 - tRNA-Ala, at 2,815,619 to 2,815,694 _RS12350 BPHYT_RS12355: BPHYT_RS12355 - glutamate--tRNA ligase, at 2,815,817 to 2,817,226 _RS12355 Position (kb) 2815 2816Strain fitness (log2 ratio) -1 0 1at 2814.657 kb on + strand, within BPHYT_RS12325at 2814.657 kb on + strand, within BPHYT_RS12325at 2814.840 kb on + strandat 2814.865 kb on + strandat 2815.088 kb on - strand, within BPHYT_RS12335at 2815.165 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
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2,814,657 + BPHYT_RS12325 0.77 +1.3
2,814,657 + BPHYT_RS12325 0.77 -0.7
2,814,840 + +1.2
2,814,865 + -0.7
2,815,088 - BPHYT_RS12335 0.14 +1.6
2,815,165 - +0.4

Or see this region's nucleotide sequence