Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4211 and BT4212 are separated by 325 nucleotides BT4212 and BT4213 are separated by 50 nucleotides
BT4211: BT4211 - putative phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 5,547,969 to 5,549,135
BT4211
BT4212: BT4212 - conserved hypothetical protein (NCBI ptt file), at 5,549,461 to 5,550,042
BT4212
BT4213: BT4213 - conserved hypothetical protein (NCBI ptt file), at 5,550,093 to 5,550,995
BT4213
Position (kb)
5549
5550
5551 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5548.466 kb on - strand, within BT4211 at 5548.539 kb on - strand, within BT4211 at 5548.553 kb on - strand, within BT4211 at 5548.557 kb on - strand, within BT4211 at 5548.717 kb on - strand, within BT4211 at 5548.717 kb on - strand, within BT4211 at 5548.941 kb on - strand, within BT4211 at 5549.149 kb on - strand at 5549.240 kb on - strand at 5549.240 kb on - strand at 5549.354 kb on + strand at 5549.397 kb on - strand at 5549.463 kb on - strand at 5549.471 kb on - strand at 5549.471 kb on - strand at 5549.510 kb on + strand at 5549.732 kb on - strand, within BT4212 at 5549.814 kb on + strand, within BT4212 at 5549.825 kb on - strand, within BT4212 at 5549.861 kb on + strand, within BT4212 at 5549.870 kb on - strand, within BT4212 at 5549.973 kb on + strand, within BT4212 at 5549.974 kb on - strand, within BT4212 at 5549.974 kb on - strand, within BT4212 at 5550.078 kb on + strand at 5550.232 kb on + strand, within BT4213 at 5550.249 kb on + strand, within BT4213 at 5550.250 kb on - strand, within BT4213 at 5550.278 kb on + strand, within BT4213 at 5550.279 kb on - strand, within BT4213 at 5550.529 kb on - strand, within BT4213 at 5550.574 kb on + strand, within BT4213 at 5550.578 kb on + strand, within BT4213 at 5550.821 kb on + strand, within BT4213 at 5550.822 kb on - strand, within BT4213 at 5550.856 kb on - strand, within BT4213 at 5550.873 kb on + strand, within BT4213 at 5550.877 kb on + strand, within BT4213 at 5550.901 kb on - strand, within BT4213 at 5550.923 kb on + strand at 5550.923 kb on + strand at 5550.925 kb on + strand at 5550.926 kb on - strand at 5550.972 kb on + strand at 5550.985 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 5,548,466 - BT4211 0.43 -1.6 5,548,539 - BT4211 0.49 -0.1 5,548,553 - BT4211 0.50 -1.1 5,548,557 - BT4211 0.50 -1.6 5,548,717 - BT4211 0.64 -4.0 5,548,717 - BT4211 0.64 -3.1 5,548,941 - BT4211 0.83 -0.1 5,549,149 - +0.9 5,549,240 - -1.6 5,549,240 - -0.8 5,549,354 + +0.3 5,549,397 - -2.3 5,549,463 - +0.2 5,549,471 - +0.9 5,549,471 - -0.1 5,549,510 + -3.1 5,549,732 - BT4212 0.47 -0.1 5,549,814 + BT4212 0.61 -3.8 5,549,825 - BT4212 0.63 -2.1 5,549,861 + BT4212 0.69 -2.2 5,549,870 - BT4212 0.70 -3.4 5,549,973 + BT4212 0.88 -1.6 5,549,974 - BT4212 0.88 -2.4 5,549,974 - BT4212 0.88 -0.1 5,550,078 + -1.1 5,550,232 + BT4213 0.15 -0.1 5,550,249 + BT4213 0.17 -2.4 5,550,250 - BT4213 0.17 -0.1 5,550,278 + BT4213 0.20 -2.9 5,550,279 - BT4213 0.21 -1.1 5,550,529 - BT4213 0.48 -2.5 5,550,574 + BT4213 0.53 -1.1 5,550,578 + BT4213 0.54 -3.1 5,550,821 + BT4213 0.81 -2.2 5,550,822 - BT4213 0.81 -0.1 5,550,856 - BT4213 0.84 -0.4 5,550,873 + BT4213 0.86 -2.6 5,550,877 + BT4213 0.87 -0.1 5,550,901 - BT4213 0.89 -1.6 5,550,923 + -2.9 5,550,923 + -1.1 5,550,925 + -2.1 5,550,926 - -1.1 5,550,972 + -0.1 5,550,985 - -0.8
Or see this region's nucleotide sequence